Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NT239_RS13255 Genome accession   NZ_CP102611
Coordinates   2847582..2848718 (+) Length   378 a.a.
NCBI ID   WP_373974587.1    Uniprot ID   -
Organism   Chitinibacter sp. SCUT-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2842582..2853718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT239_RS13225 (NT239_13240) cutA 2843390..2843740 (-) 351 WP_373974581.1 divalent-cation tolerance protein CutA -
  NT239_RS13230 (NT239_13245) - 2843756..2844199 (+) 444 WP_373974582.1 FxsA family protein -
  NT239_RS13235 (NT239_13250) - 2844288..2844875 (-) 588 WP_373974583.1 YggT family protein -
  NT239_RS13240 (NT239_13255) proC 2844906..2845724 (-) 819 WP_373974584.1 pyrroline-5-carboxylate reductase -
  NT239_RS13245 (NT239_13260) - 2845721..2846431 (-) 711 WP_373974585.1 YggS family pyridoxal phosphate-dependent enzyme -
  NT239_RS13250 (NT239_13265) pilT 2846518..2847561 (+) 1044 WP_373974586.1 type IV pilus twitching motility protein PilT Machinery gene
  NT239_RS13255 (NT239_13270) pilU 2847582..2848718 (+) 1137 WP_373974587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NT239_RS13260 (NT239_13275) - 2848745..2850973 (+) 2229 WP_373974588.1 FimV family protein -
  NT239_RS13265 (NT239_13280) aceB 2851044..2852630 (-) 1587 WP_373974589.1 malate synthase A -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42582.78 Da        Isoelectric Point: 6.0954

>NTDB_id=718156 NT239_RS13255 WP_373974587.1 2847582..2848718(+) (pilU) [Chitinibacter sp. SCUT-21]
MEKEQAAKFMHDLLRHMRSKNASDLFITVDFPPAMKIDGRVTPVSNQLLTAQHTKELARAIMNDRQAEDFEANKECNFAI
SPGSMGRFRVNAFMQQGRVGMVLRTINSEIPKLSELNLPPVLEDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENAYD
HIITIEDPIEYVHEHKNSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLKAFVSQRLVPHLSGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLFEQDRISYEDALRNADSVNDLRLQIKLNSSDAKQRDVLAGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=718156 NT239_RS13255 WP_373974587.1 2847582..2848718(+) (pilU) [Chitinibacter sp. SCUT-21]
ATGGAAAAAGAACAAGCCGCCAAGTTTATGCATGATTTACTGCGCCATATGCGCAGTAAAAATGCGTCCGATTTATTTAT
TACGGTTGATTTTCCGCCAGCGATGAAAATCGATGGTCGAGTAACGCCAGTATCGAATCAGCTATTAACTGCGCAGCATA
CCAAAGAGCTGGCGCGGGCGATTATGAACGATCGCCAAGCCGAAGATTTTGAAGCAAATAAAGAATGTAATTTTGCGATT
AGCCCGGGCAGTATGGGGCGTTTTCGCGTGAATGCGTTTATGCAGCAAGGCCGTGTAGGCATGGTGCTGCGGACGATTAA
TTCCGAAATTCCAAAGTTGTCTGAGTTAAATCTGCCGCCGGTGTTGGAAGACATTGCGATGACCAAACGTGGTCTGGTGA
TTTTCGTTGGTGGAACAGGCTCGGGTAAATCAACTTCGCTCGCGGCGATGATTGGCCATCGTAATGAAAATGCGTACGAC
CATATCATCACGATTGAAGACCCTATCGAGTATGTTCACGAGCATAAAAACTCGATCATTACCCAGCGCGAAGTTGGCGT
TGATACCGACTCGTGGATGGCGGCACTGAAAAACACGCTGCGTCAGGCGCCCGATGTGATTTTGATCGGTGAGATTCGCG
ACCGAGAAACGATGGATTACGCGATTGCATTTGCTGAGACCGGCCACCTATGTATGGCAACCTTGCATGCCAACTCGTCC
AATCAGGCACTCGATCGGATTATTAACTTCTTCCCCGAAGAGCGCCGGCAACAATTGTTGATGGATTTGTCCTTGAACTT
AAAAGCCTTTGTTTCTCAGCGCTTGGTGCCGCATTTGTCAGGCAAAGGTCGCGTTGCTGCGGTTGAGGTGATGCTGAATT
CACCATTGATTTCTGAGTTGATTTTCAAAGGCGAAGTGCATGAAATCAAAGAGATTATGAAAAAATCTCGTGAATTGGGC
ATGCAGACTTTCGATCAGTCACTGTTCGATTTGTTTGAGCAAGACCGGATTTCATATGAAGATGCGCTGCGAAATGCAGA
CTCGGTCAACGATCTGCGTTTGCAGATTAAGCTCAATAGCTCAGACGCAAAACAGCGCGATGTTTTAGCTGGCTTAGACC
ACTTGGATATCGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

92.063

0.582
  pilU Acinetobacter baylyi ADP1

58.824

94.444

0.556
  pilU Vibrio cholerae strain A1552

54.674

93.386

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.97

88.624

0.407
  pilT Acinetobacter baylyi ADP1

42.433

89.153

0.378
  pilT Acinetobacter nosocomialis M2

42.296

87.566

0.37
  pilT Acinetobacter baumannii D1279779

42.296

87.566

0.37
  pilT Acinetobacter baumannii strain A118

42.296

87.566

0.37
  pilT Pseudomonas aeruginosa PAK

41.493

88.624

0.368
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Pseudomonas stutzeri DSM 10701

41.194

88.624

0.365