Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KQ693_RS03380 Genome accession   NZ_CP102606
Coordinates   602544..603632 (-) Length   362 a.a.
NCBI ID   WP_219760531.1    Uniprot ID   -
Organism   Thermus sp. PS18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 597544..608632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ693_RS03365 (KQ693_03365) - 599471..600100 (-) 630 WP_015716085.1 histidine phosphatase family protein -
  KQ693_RS03370 (KQ693_03370) purM 600097..601098 (-) 1002 WP_267262350.1 phosphoribosylformylglycinamidine cyclo-ligase -
  KQ693_RS03375 (KQ693_03375) gatB 601104..602513 (-) 1410 WP_267261924.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  KQ693_RS03380 (KQ693_03380) pilT 602544..603632 (-) 1089 WP_219760531.1 type IV pilus twitching motility protein PilT Machinery gene
  KQ693_RS03385 (KQ693_03385) pilF 603644..606310 (-) 2667 WP_219760532.1 type IV pilus assembly ATPase PilB Machinery gene
  KQ693_RS03390 (KQ693_03390) - 606307..606789 (-) 483 WP_267261925.1 YqeG family HAD IIIA-type phosphatase -
  KQ693_RS03395 (KQ693_03395) pgeF 606824..607549 (-) 726 WP_219760534.1 peptidoglycan editing factor PgeF -
  KQ693_RS03400 (KQ693_03400) - 607629..608033 (+) 405 WP_219760535.1 DUF4395 family protein -
  KQ693_RS03405 (KQ693_03405) ribH 608020..608493 (-) 474 WP_267261926.1 6,7-dimethyl-8-ribityllumazine synthase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40113.41 Da        Isoelectric Point: 7.2368

>NTDB_id=718110 KQ693_RS03380 WP_219760531.1 602544..603632(-) (pilT) [Thermus sp. PS18]
MAKALDIVDLLTLAVERGASDLVITVGLPPMIKVDGEFHPTEYETLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPATIKSFEELGLPKNIAEIALSPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKKAIVNQREIGSDTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLSRAVDPKEFMRLAGVQEGSKRP

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=718110 KQ693_RS03380 WP_219760531.1 602544..603632(-) (pilT) [Thermus sp. PS18]
ATGGCCAAAGCACTGGATATCGTGGATCTTCTGACCCTGGCTGTAGAGCGGGGGGCCAGCGACCTGGTGATCACCGTGGG
CCTTCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGACCCTCTCCCCCCAGGACACCCGCCGGC
TCATGTACGCCCTCATGGATGAGAAGCAGCAGCGGATCTTTGAGGAGGAAAAGGAGCTGGACTTCTCCTTCAGCCTCCCG
GGAAAAGGGCGCTACCGGGTGAACGTCTTCCTGCAACGGGGAAGCGTGGGAGGGGTCTTAAGAGTAGTGCCCGCCACCAT
CAAGAGCTTTGAGGAGCTGGGCCTGCCCAAGAACATCGCCGAGATCGCCCTTTCTCCCAGGGGCCTGGTCCTGGTCACGG
GGCCCACCGGGTCGGGGAAGAGCACCACCCTGGCCTCCATGATCGACTACATCAACGAACGCAAGCCCGTGCACATCGTG
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAGAAGGCCATCGTCAACCAGCGGGAGATCGGCTCTGATACCCA
CGGCTTCCACAAGGCGCTTAGAAGCGTTCTCCGCCAGGCCCCGGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAAA
CCATCGCCGCTGCCATCACCGCTGCGGAAACCGGGCACTTGGTCATGGGTACCCTGCACACCAACTCCGCCCCCGAGACC
ATCGACCGCATCATCGACGTCTTCCCGGAAAATCAGCAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCGGT
GCTTACCCAGCAGCTTCTGCCCAAGGCCTTCGGGGGCGGAAGGGTCTTGGCCTACGAGCTCATGATCGCCACCCCTGCGG
TAAGGGCTTTGATCCGGGAGGGCAAAAGCCACCAGCTCCGAAGCGTCATCCAGACCGGGGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTGGCGGATCTCTACCGGCGCAAGCTGATCACCTACGAGATGGGCTTGAGCCGGGCGGTGGACCCCAA
GGAGTTCATGCGCCTGGCTGGGGTGCAGGAGGGGAGCAAGCGCCCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.989

97.514

0.663
  pilT Acinetobacter baumannii D1279779

54.046

95.58

0.517
  pilT Acinetobacter baumannii strain A118

54.046

95.58

0.517
  pilT Acinetobacter nosocomialis M2

54.046

95.58

0.517
  pilT Pseudomonas stutzeri DSM 10701

53.913

95.304

0.514
  pilT Pseudomonas aeruginosa PAK

53.913

95.304

0.514
  pilT Acinetobacter baylyi ADP1

53.913

95.304

0.514
  pilT Neisseria meningitidis 8013

52.647

93.923

0.494
  pilT Legionella pneumophila strain Lp02

51.304

95.304

0.489
  pilT Legionella pneumophila strain ERS1305867

51.304

95.304

0.489
  pilT Neisseria gonorrhoeae MS11

51.917

93.646

0.486
  pilT Vibrio cholerae strain A1552

52.424

91.16

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.424

91.16

0.478
  pilU Pseudomonas stutzeri DSM 10701

47.522

94.751

0.45
  pilU Vibrio cholerae strain A1552

44.611

92.265

0.412
  pilU Acinetobacter baylyi ADP1

40.947

99.171

0.406