Detailed information NTG0718111

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: KQ693_RS03385
• Genome accession: NZ_CP102606
• Coordinates: 603644..606310 (-)
• Length: 888 a.a.
• NCBI ID: WP_219760532.1
• Uniprot ID: -
• Organism: Thermus sp. PS18
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 598644..611310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KQ693_RS03365 (KQ693_03365)	-	599471..600100 (-)	630	WP_015716085.1	histidine phosphatase family protein	-
KQ693_RS03370 (KQ693_03370)	purM	600097..601098 (-)	1002	WP_267262350.1	phosphoribosylformylglycinamidine cyclo-ligase	-
KQ693_RS03375 (KQ693_03375)	gatB	601104..602513 (-)	1410	WP_267261924.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
KQ693_RS03380 (KQ693_03380)	pilT	602544..603632 (-)	1089	WP_219760531.1	type IV pilus twitching motility protein PilT	Machinery gene
KQ693_RS03385 (KQ693_03385)	pilF	603644..606310 (-)	2667	WP_219760532.1	type IV pilus assembly ATPase PilB	Machinery gene
KQ693_RS03390 (KQ693_03390)	-	606307..606789 (-)	483	WP_267261925.1	YqeG family HAD IIIA-type phosphatase	-
KQ693_RS03395 (KQ693_03395)	pgeF	606824..607549 (-)	726	WP_219760534.1	peptidoglycan editing factor PgeF	-
KQ693_RS03400 (KQ693_03400)	-	607629..608033 (+)	405	WP_219760535.1	DUF4395 family protein	-
KQ693_RS03405 (KQ693_03405)	ribH	608020..608493 (-)	474	WP_267261926.1	6,7-dimethyl-8-ribityllumazine synthase	-
KQ693_RS03410 (KQ693_03410)	crcB	608514..608891 (+)	378	WP_038071939.1	fluoride efflux transporter CrcB	-
KQ693_RS03415 (KQ693_03415)	-	608896..609228 (+)	333	WP_015716095.1	DUF190 domain-containing protein	-
KQ693_RS03420 (KQ693_03420)	-	609273..609887 (+)	615	WP_267261927.1	sulfite oxidase-like oxidoreductase	-
KQ693_RS03425 (KQ693_03425)	-	609884..610153 (+)	270	WP_015716097.1	hypothetical protein	-
KQ693_RS03430 (KQ693_03430)	-	610129..610932 (-)	804	WP_267261928.1	histidinol-phosphatase HisJ family protein	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 97960.03 Da
• Isoelectric Point: 5.0516

>NTDB_id=718111 KQ693_RS03385 WP_219760532.1 603644..606310(-) (pilF) [Thermus sp. PS18]
MSVLTIGDKRLGAILLDAGLLTDEELQMALEKHREVGGSLAEVIVDSGLLSERRIAQAIEDHFGIPLVELHTLEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGMVVEPYQATKSAFLYALARNYPELNLPLPPPPSG
PSREELKVGELLVEKGLLDRSTLEEALVEQEKTGDLLGRILVRKGLPEEVLYRVLAGQKEMEFLPSTQDLFPDPAAASLL
LRSDALRYSAVPVGFNNGEVEVVLADPRHKEAVAELLGRPARFLLTLPKEWEALFHQAYPEKSRLGEVLVQEGRLSREDL
REALEVQRRLPKAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLVASGKLKPEALAQAVAAQLGYAYINPEENPP
DPGAALLLPEDLARRYGVFPHRLEGKTLVLLMKDPRNILALDDVRLALKRKGLAYEVQPAVATEAAITKLIERFYGKEEL
GEIAKELSKGYQEEEAVTPELDESAAQKFVKQVIREAFLQDASDIHIEPRQSDVLVRLRIDGTLRQYTTLPKGALNSIIS
VVKIMGGLNIAERRLPQDGRVRYREGSIDLDLRLSTLPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVISKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRRICDHCKVEVKPDPEVLRRLGLS
QEEIQGAKLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARKKGMKTLREDGIYKAFQGITTLE
EVLARTIE

Nucleotide

• Length: 2667 bp

>NTDB_id=718111 KQ693_RS03385 WP_219760532.1 603644..606310(-) (pilF) [Thermus sp. PS18]
ATGAGCGTGCTCACCATCGGGGACAAACGGCTAGGTGCCATCCTATTGGATGCCGGCCTCCTCACCGACGAGGAGCTGCA
GATGGCCCTGGAAAAGCACCGGGAGGTAGGGGGTTCCTTGGCCGAGGTCATCGTGGACTCGGGGCTGCTTTCGGAAAGGC
GGATCGCCCAGGCCATCGAGGACCACTTCGGCATCCCCCTGGTGGAGCTCCACACCCTAGAGATCCCCCCCAAGGTGAAG
GCCCTCCTGCCGGCGGAGAAGGCCAAGGAGCTCCAGGCCATCCCCTTCGCCCTGGACGAGGAGGCAGGGGTGGTGCGGGT
GGCCTTCGTGAACCCCCTGGACACCCTGGCCCTCGAGGAGGTGGAGGACCTCACCGGGATGGTGGTGGAACCCTACCAGG
CCACCAAGAGCGCCTTCCTCTACGCCCTGGCCAGGAACTACCCCGAGCTCAACCTGCCCCTACCCCCTCCCCCCAGCGGC
CCCAGCCGGGAGGAGCTGAAGGTGGGCGAGCTTCTGGTGGAGAAGGGTCTCCTGGACCGGAGTACCCTGGAGGAAGCCCT
GGTAGAGCAGGAAAAGACCGGGGATCTCCTGGGCCGCATCCTGGTGCGCAAGGGGCTACCCGAGGAAGTCCTCTACCGGG
TTTTGGCGGGGCAGAAGGAAATGGAGTTTCTCCCCTCCACCCAGGACCTATTCCCCGACCCCGCCGCCGCCAGCCTTCTC
CTCCGCTCGGATGCCCTGCGCTATAGCGCCGTACCCGTGGGATTCAACAACGGGGAGGTGGAGGTGGTCCTCGCCGATCC
CCGGCACAAGGAAGCGGTGGCGGAGCTCCTTGGGCGCCCCGCCCGTTTCCTCCTCACCCTTCCCAAGGAGTGGGAAGCCC
TTTTCCACCAGGCCTATCCGGAAAAAAGCCGTCTGGGGGAGGTCCTGGTCCAGGAGGGGCGCCTGAGCCGCGAGGATTTA
AGGGAGGCCCTCGAGGTGCAAAGGCGCCTGCCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTGGGCTTGGCCCG
GCCCGAGGACGTGGAGGAAGCCTTGAAGAAGCAACGCCAGGGTGGGGGCCGCCTCGAGGACACCTTGGTGGCCTCGGGCA
AGCTCAAACCCGAGGCCCTGGCCCAGGCGGTGGCCGCCCAGCTGGGCTATGCCTACATCAACCCCGAGGAGAACCCCCCG
GACCCCGGGGCCGCCCTCCTCCTCCCCGAGGACCTGGCCCGGCGGTACGGGGTCTTCCCCCACCGCCTGGAGGGCAAGAC
CCTGGTCCTCCTCATGAAAGACCCCAGGAACATCCTGGCCCTGGATGACGTACGCCTGGCCCTGAAGCGCAAGGGCCTGG
CCTACGAGGTTCAGCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGAGGAGCTG
GGCGAGATCGCCAAGGAGCTGTCCAAGGGCTACCAGGAGGAGGAGGCCGTCACCCCCGAGCTGGACGAAAGCGCCGCCCA
GAAGTTCGTCAAGCAGGTGATCCGGGAGGCCTTCCTGCAGGATGCCTCCGACATCCACATAGAACCCCGGCAGTCGGATG
TGCTGGTGCGCCTCCGCATCGACGGCACCCTGCGCCAGTACACCACCCTGCCCAAGGGGGCCCTAAACTCCATCATCAGC
GTGGTCAAGATCATGGGCGGCCTCAACATCGCCGAAAGGCGCCTGCCCCAGGACGGGCGGGTGCGCTACCGGGAAGGTTC
CATCGACCTGGACCTCCGCCTCTCCACCCTGCCCACGGTCTACGGGGAAAAGGCGGTGATGCGCCTTTTGAAGAAGGCCG
CCGACATCCCCGAGATCGAGGGCCTGGGCTTCGCCCCCGGGGTCTTTGAGCGCTTCCAGGAGGTGATCTCCAAGCCCTAC
GGCATCTTCCTCATCACCGGGCCCACCGGAAGCGGCAAAAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCC
CGACAAGAACACCCAGACCATCGAGGATCCCGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCCCAGG
CGGGGCTCACCTTCGCCCGGGCCCTCAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGAC
TCGGAAACCGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTGGTCATCGCCACCCTGCACACCAACGACGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCTTTTCAACATCTCCGCCGCCCTCATCGGGGTTCTCTCCCAGC
GCCTGGTGCGGAGGATCTGCGACCACTGCAAGGTGGAGGTGAAGCCCGACCCCGAGGTCCTGCGGCGCCTGGGCCTTTCC
CAGGAGGAGATCCAAGGAGCCAAGCTCTACAAGGGCATGGGGTGCGAGCGCTGCGGGGGAACGGGCTATAAGGGGCGTTA
CGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCATGCCATCGTGGCCGGAAAGTCGGCCACCGAGATCAAGG
AGATCGCCAGGAAAAAGGGCATGAAAACCCTGCGGGAGGACGGCATCTACAAGGCCTTCCAGGGAATCACCACCCTCGAG
GAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (512-778)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-148)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (172-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	87.289	100	0.874
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.517	100	0.566