Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NUG21_RS06790 Genome accession   NZ_CP102594
Coordinates   1624947..1625984 (+) Length   345 a.a.
NCBI ID   WP_160946406.1    Uniprot ID   -
Organism   Xanthomonas sp. CFBP 8445     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1619947..1630984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG21_RS06765 (NUG21_06765) - 1621241..1621693 (-) 453 WP_263392642.1 DUF4426 domain-containing protein -
  NUG21_RS06775 (NUG21_06775) proC 1621852..1622697 (-) 846 WP_263392643.1 pyrroline-5-carboxylate reductase -
  NUG21_RS06780 (NUG21_06780) - 1622829..1623857 (-) 1029 WP_160946530.1 hypothetical protein -
  NUG21_RS06785 (NUG21_06785) - 1624174..1624824 (-) 651 WP_263393047.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUG21_RS06790 (NUG21_06790) pilT 1624947..1625984 (+) 1038 WP_160946406.1 type IV pilus twitching motility protein PilT Machinery gene
  NUG21_RS06795 (NUG21_06795) pilU 1626103..1627233 (+) 1131 WP_160946405.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NUG21_RS06800 (NUG21_06800) - 1627390..1628670 (+) 1281 WP_263392644.1 PQQ-like beta-propeller repeat protein -
  NUG21_RS06805 (NUG21_06805) - 1628667..1630166 (+) 1500 WP_263392645.1 diguanylate cyclase -
  NUG21_RS06810 (NUG21_06810) - 1630210..1630380 (+) 171 WP_263392646.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38551.20 Da        Isoelectric Point: 6.6653

>NTDB_id=717991 NUG21_RS06790 WP_160946406.1 1624947..1625984(+) (pilT) [Xanthomonas sp. CFBP 8445]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQSGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=717991 NUG21_RS06790 WP_160946406.1 1624947..1625984(+) (pilT) [Xanthomonas sp. CFBP 8445]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAGGCGTCCGACCTGCATCTGTCCGCGGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTCCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTGCACGCGCTTGTCTACGACA
TCATGTCCGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAAGTCGACTTCTCCTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAATCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGGAAGTGCTGACCCTGGA
AGACCTGGGCTGTCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAGTACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAGATCATGGTCGGCACCCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGAGCGGCATGCAGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGTTGTTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.351

98.261

0.78
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

66.276

98.841

0.655
  pilT Neisseria gonorrhoeae MS11

65.982

98.841

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397