Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NUG21_RS06795 Genome accession   NZ_CP102594
Coordinates   1626103..1627233 (+) Length   376 a.a.
NCBI ID   WP_160946405.1    Uniprot ID   -
Organism   Xanthomonas sp. CFBP 8445     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1621103..1632233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG21_RS06765 (NUG21_06765) - 1621241..1621693 (-) 453 WP_263392642.1 DUF4426 domain-containing protein -
  NUG21_RS06775 (NUG21_06775) proC 1621852..1622697 (-) 846 WP_263392643.1 pyrroline-5-carboxylate reductase -
  NUG21_RS06780 (NUG21_06780) - 1622829..1623857 (-) 1029 WP_160946530.1 hypothetical protein -
  NUG21_RS06785 (NUG21_06785) - 1624174..1624824 (-) 651 WP_263393047.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUG21_RS06790 (NUG21_06790) pilT 1624947..1625984 (+) 1038 WP_160946406.1 type IV pilus twitching motility protein PilT Machinery gene
  NUG21_RS06795 (NUG21_06795) pilU 1626103..1627233 (+) 1131 WP_160946405.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NUG21_RS06800 (NUG21_06800) - 1627390..1628670 (+) 1281 WP_263392644.1 PQQ-like beta-propeller repeat protein -
  NUG21_RS06805 (NUG21_06805) - 1628667..1630166 (+) 1500 WP_263392645.1 diguanylate cyclase -
  NUG21_RS06810 (NUG21_06810) - 1630210..1630380 (+) 171 WP_263392646.1 hypothetical protein -
  NUG21_RS06815 (NUG21_06815) - 1630532..1631248 (+) 717 WP_263392647.1 YitT family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41922.19 Da        Isoelectric Point: 6.8811

>NTDB_id=717992 NUG21_RS06795 WP_160946405.1 1626103..1627233(+) (pilU) [Xanthomonas sp. CFBP 8445]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=717992 NUG21_RS06795 WP_160946405.1 1626103..1627233(+) (pilU) [Xanthomonas sp. CFBP 8445]
ATGAGCACCATCGACTTCACCTCCTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTCCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CAGCGTGGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGATCACGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGGGTCATCAACTTCTTCCCCGAAGACCGCCGCAGCCAGTTGCTGATGGACCTGTCGCTGAACCTGCGCGGGGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCCATGGAGATCATGCTCGGCACGCCGTTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGATGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCACGCACCCTGGCGCAGGGCCTGGACGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42

93.085

0.391
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37