Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NUG20_RS15130 Genome accession   NZ_CP102592
Coordinates   3601304..3602341 (-) Length   345 a.a.
NCBI ID   WP_185815705.1    Uniprot ID   -
Organism   Xanthomonas sp. CFBP 8443     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3596304..3607341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG20_RS15110 (NUG20_15115) - 3596466..3596990 (-) 525 WP_263395268.1 MFS transporter permease -
  NUG20_RS15115 (NUG20_15120) - 3597101..3598597 (-) 1497 WP_263395269.1 diguanylate cyclase -
  NUG20_RS15120 (NUG20_15125) - 3598594..3599925 (-) 1332 WP_263395270.1 PQQ-binding-like beta-propeller repeat protein -
  NUG20_RS15125 (NUG20_15130) pilU 3600014..3601144 (-) 1131 WP_263112813.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NUG20_RS15130 (NUG20_15135) pilT 3601304..3602341 (-) 1038 WP_185815705.1 type IV pilus twitching motility protein PilT Machinery gene
  NUG20_RS15135 (NUG20_15140) - 3602430..3603134 (+) 705 WP_263395271.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUG20_RS15140 (NUG20_15145) proC 3603209..3604060 (+) 852 WP_263395272.1 pyrroline-5-carboxylate reductase -
  NUG20_RS15145 (NUG20_15150) - 3604075..3604530 (+) 456 WP_263395273.1 DUF4426 domain-containing protein -
  NUG20_RS15150 (NUG20_15155) soxR 3604659..3605096 (-) 438 WP_263395274.1 redox-sensitive transcriptional activator SoxR -
  NUG20_RS15155 (NUG20_15160) - 3605251..3606621 (+) 1371 WP_263398495.1 MFS transporter -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38551.20 Da        Isoelectric Point: 6.6653

>NTDB_id=717974 NUG20_RS15130 WP_185815705.1 3601304..3602341(-) (pilT) [Xanthomonas sp. CFBP 8443]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQTGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRVFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=717974 NUG20_RS15130 WP_185815705.1 3601304..3602341(-) (pilT) [Xanthomonas sp. CFBP 8443]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAAGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAGTTCCTGGAAGTCGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGGAAGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCCATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGGGGCCGCACCGCGGCGTGGGAAATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGACCGGCATGCAGACCTTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGGTCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.351

98.261

0.78
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394