Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NUG20_RS15125 Genome accession   NZ_CP102592
Coordinates   3600014..3601144 (-) Length   376 a.a.
NCBI ID   WP_263112813.1    Uniprot ID   -
Organism   Xanthomonas sp. CFBP 8443     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3595014..3606144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG20_RS15105 (NUG20_15110) - 3595614..3596330 (-) 717 WP_263395267.1 YitT family protein -
  NUG20_RS15110 (NUG20_15115) - 3596466..3596990 (-) 525 WP_263395268.1 MFS transporter permease -
  NUG20_RS15115 (NUG20_15120) - 3597101..3598597 (-) 1497 WP_263395269.1 diguanylate cyclase -
  NUG20_RS15120 (NUG20_15125) - 3598594..3599925 (-) 1332 WP_263395270.1 PQQ-binding-like beta-propeller repeat protein -
  NUG20_RS15125 (NUG20_15130) pilU 3600014..3601144 (-) 1131 WP_263112813.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NUG20_RS15130 (NUG20_15135) pilT 3601304..3602341 (-) 1038 WP_185815705.1 type IV pilus twitching motility protein PilT Machinery gene
  NUG20_RS15135 (NUG20_15140) - 3602430..3603134 (+) 705 WP_263395271.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUG20_RS15140 (NUG20_15145) proC 3603209..3604060 (+) 852 WP_263395272.1 pyrroline-5-carboxylate reductase -
  NUG20_RS15145 (NUG20_15150) - 3604075..3604530 (+) 456 WP_263395273.1 DUF4426 domain-containing protein -
  NUG20_RS15150 (NUG20_15155) soxR 3604659..3605096 (-) 438 WP_263395274.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41884.20 Da        Isoelectric Point: 6.8811

>NTDB_id=717973 NUG20_RS15125 WP_263112813.1 3600014..3601144(-) (pilU) [Xanthomonas sp. CFBP 8443]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=717973 NUG20_RS15125 WP_263112813.1 3600014..3601144(-) (pilU) [Xanthomonas sp. CFBP 8443]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACTCCGATCACGCAGACGCCGCTGACCTCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTGGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAAACCGGCCACTTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCGGCCAGTTGCTGATGGACCTGTCGCTGAACCTGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACTTGGGCATGAAGACTTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGATGCCAAGACCCTGGCGCAGGGCATGGACGGAGTGGAGATCGCCG
AGGTGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.854

98.138

0.646
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37