Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQE15_RS23090 Genome accession   NZ_CP102486
Coordinates   4634706..4635749 (-) Length   347 a.a.
NCBI ID   WP_265945170.1    Uniprot ID   -
Organism   Dechloromonas sp. A34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4629706..4640749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQE15_RS23060 - 4629812..4630255 (+) 444 WP_265945160.1 c-type cytochrome -
  NQE15_RS23065 - 4630382..4630702 (-) 321 WP_265945162.1 hypothetical protein -
  NQE15_RS23070 - 4630843..4632834 (-) 1992 WP_265945164.1 UvrD-helicase domain-containing protein -
  NQE15_RS23075 - 4632904..4633221 (-) 318 WP_265945166.1 hypothetical protein -
  NQE15_RS23080 - 4633272..4633958 (-) 687 WP_265945168.1 DUF4197 domain-containing protein -
  NQE15_RS23085 - 4633984..4634673 (-) 690 Protein_4537 ATPase, T2SS/T4P/T4SS family -
  NQE15_RS23090 pilT 4634706..4635749 (-) 1044 WP_265945170.1 type IV pilus twitching motility protein PilT Machinery gene
  NQE15_RS23095 - 4635806..4636495 (+) 690 WP_265945172.1 YggS family pyridoxal phosphate-dependent enzyme -
  NQE15_RS23100 proC 4636492..4637304 (+) 813 WP_265945174.1 pyrroline-5-carboxylate reductase -
  NQE15_RS23105 - 4637307..4637840 (+) 534 WP_265945176.1 YggT family protein -
  NQE15_RS23110 - 4637837..4638133 (+) 297 WP_265945178.1 DUF167 domain-containing protein -
  NQE15_RS23115 comE 4638166..4638453 (-) 288 WP_265945180.1 ComEA family DNA-binding protein Machinery gene
  NQE15_RS23120 - 4638559..4639503 (-) 945 WP_323054922.1 dihydroorotase -
  NQE15_RS23975 - 4639568..4639804 (-) 237 WP_323054923.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38461.11 Da        Isoelectric Point: 6.8848

>NTDB_id=717260 NQE15_RS23090 WP_265945170.1 4634706..4635749(-) (pilT) [Dechloromonas sp. A34]
MDITELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPAMEHKDVHGMVYDIMNDGQRKIYEETLECDFSFEIPNLAR
FRVNAFNQQRGAGAVFRTIPSKVLTLEELNCPKIFKDISEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENDYGHILTVED
PIEFVHEAKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNFGMQTLDQN
LLEMVRRNIVSSAEARNKAANKDAFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=717260 NQE15_RS23090 WP_265945170.1 4634706..4635749(-) (pilT) [Dechloromonas sp. A34]
ATGGACATCACCGAACTGCTGGCCTTCAGCGTCAAGAACAAGGCCTCCGATCTTCATTTGTCCTCCGGCTTGCCACCGAT
GATTCGCGTCCATGGCGATGTCCGTCGCATCAATCTGCCGGCCATGGAGCACAAGGATGTGCACGGCATGGTGTATGACA
TCATGAACGATGGTCAGCGCAAGATTTATGAAGAAACGCTGGAGTGCGACTTTTCATTTGAAATTCCCAATCTGGCGCGC
TTCCGGGTCAATGCCTTCAATCAGCAGCGGGGAGCCGGTGCCGTCTTCCGGACCATTCCCTCCAAGGTGCTGACCCTGGA
GGAGTTGAATTGCCCGAAAATCTTCAAGGACATTTCCGAGTACCCACGCGGCATCGTGCTGGTTACCGGGCCAACCGGTT
CCGGCAAATCGACGACGCTGGCCGCCATGGTCAACCATGTCAATGAAAACGACTACGGGCATATCCTGACCGTCGAGGAT
CCGATCGAATTCGTCCATGAGGCTAAAAAGTGCCTGATCAACCAGCGTGAAGTCGGGCCGCACACGCTGTCCTTTTCCAA
CGCCCTGCGCTCGGCCTTGCGCGAAGACCCGGACGTCATTCTGGTCGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAAACCGGTCACCTGGTGTTCGGGACGCTGCATACCTCGTCGGCGGCGAAGACGGTGGACCGCATC
GTCGACGTTTTCCCGGCTGCGGAAAAGGAAATGGTTCGTTCGATGCTCTCCGAATCGCTGCGGGCCGTCATTTCGCAGAC
ATTGTTGAAGACCAAGGATGGTCAGGGCCGGGTCGCGGCCCATGAAATCATGATCGGCACGCCAGCCATCCGCAATCTGA
TCCGCGAGAACAAGGTGGCACAGATGTATTCGGCGATCCAGACCGGCCAGAATTTCGGGATGCAGACCCTCGACCAGAAT
CTGCTGGAAATGGTCCGTCGCAACATCGTTTCCAGCGCCGAGGCGCGTAACAAGGCTGCCAACAAGGACGCCTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.841

99.424

0.784
  pilT Acinetobacter baumannii D1279779

78.841

99.424

0.784
  pilT Acinetobacter baumannii strain A118

78.841

99.424

0.784
  pilT Acinetobacter baylyi ADP1

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Neisseria meningitidis 8013

72.464

99.424

0.72
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

72.174

99.424

0.718
  pilT Vibrio cholerae strain A1552

69.527

97.406

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.527

97.406

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

41.003

97.695

0.401
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392