Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NUT31_RS13015 Genome accession   NZ_CP102454
Coordinates   2918622..2919656 (+) Length   344 a.a.
NCBI ID   WP_005305010.1    Uniprot ID   -
Organism   Aeromonas sp. BC14     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2913622..2924656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUT31_RS12985 (NUT31_12980) - 2915301..2915612 (+) 312 WP_194759814.1 hypothetical protein -
  NUT31_RS12990 (NUT31_12985) - 2915669..2916088 (-) 420 WP_194759815.1 DUF4426 domain-containing protein -
  NUT31_RS12995 (NUT31_12990) yggU 2916107..2916406 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  NUT31_RS13000 (NUT31_12995) - 2916406..2916957 (-) 552 WP_268013772.1 YggT family protein -
  NUT31_RS13005 (NUT31_13000) proC 2916998..2917822 (-) 825 WP_194759816.1 pyrroline-5-carboxylate reductase -
  NUT31_RS13010 (NUT31_13005) - 2917881..2918582 (-) 702 WP_187268865.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUT31_RS13015 (NUT31_13010) pilT 2918622..2919656 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NUT31_RS13020 (NUT31_13015) pilU 2919685..2920794 (+) 1110 WP_268013774.1 type IVa pilus ATPase TapU Machinery gene
  NUT31_RS13025 (NUT31_13020) yaaA 2920826..2921599 (+) 774 WP_268013775.1 peroxide stress protein YaaA -
  NUT31_RS13030 (NUT31_13025) srmB 2921776..2922999 (-) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  NUT31_RS13035 (NUT31_13030) - 2923128..2923838 (+) 711 WP_268013776.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38319.12 Da        Isoelectric Point: 6.7439

>NTDB_id=717123 NUT31_RS13015 WP_005305010.1 2918622..2919656(+) (pilT) [Aeromonas sp. BC14]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=717123 NUT31_RS13015 WP_005305010.1 2918622..2919656(+) (pilT) [Aeromonas sp. BC14]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCAAACCTCGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCCGTGTTTCGGACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTGGACGCCCCGGAGATCTTTCGCAAGATTGCCGAATACCCCCGCGGTCTGGTACTGGTGACCGGCCCGACCGGCT
CGGGCAAATCCACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTGGAAACCATTCGTCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAGTCGCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCCGCCGTACGAAACCTCATCC
GGGAAGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGCGGCATGGTGGCGGCGCTCGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407