Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NUT31_RS13020 Genome accession   NZ_CP102454
Coordinates   2919685..2920794 (+) Length   369 a.a.
NCBI ID   WP_268013774.1    Uniprot ID   -
Organism   Aeromonas sp. BC14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2914685..2925794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUT31_RS12985 (NUT31_12980) - 2915301..2915612 (+) 312 WP_194759814.1 hypothetical protein -
  NUT31_RS12990 (NUT31_12985) - 2915669..2916088 (-) 420 WP_194759815.1 DUF4426 domain-containing protein -
  NUT31_RS12995 (NUT31_12990) yggU 2916107..2916406 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  NUT31_RS13000 (NUT31_12995) - 2916406..2916957 (-) 552 WP_268013772.1 YggT family protein -
  NUT31_RS13005 (NUT31_13000) proC 2916998..2917822 (-) 825 WP_194759816.1 pyrroline-5-carboxylate reductase -
  NUT31_RS13010 (NUT31_13005) - 2917881..2918582 (-) 702 WP_187268865.1 YggS family pyridoxal phosphate-dependent enzyme -
  NUT31_RS13015 (NUT31_13010) pilT 2918622..2919656 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NUT31_RS13020 (NUT31_13015) pilU 2919685..2920794 (+) 1110 WP_268013774.1 type IVa pilus ATPase TapU Machinery gene
  NUT31_RS13025 (NUT31_13020) yaaA 2920826..2921599 (+) 774 WP_268013775.1 peroxide stress protein YaaA -
  NUT31_RS13030 (NUT31_13025) srmB 2921776..2922999 (-) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  NUT31_RS13035 (NUT31_13030) - 2923128..2923838 (+) 711 WP_268013776.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NUT31_RS13040 (NUT31_13035) brnQ 2924048..2925337 (+) 1290 WP_187268869.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40905.02 Da        Isoelectric Point: 6.3880

>NTDB_id=717124 NUT31_RS13020 WP_268013774.1 2919685..2920794(+) (pilU) [Aeromonas sp. BC14]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLTEAPLDKAGALALVKETLSPEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIEARIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHMDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEVHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=717124 NUT31_RS13020 WP_268013774.1 2919685..2920794(+) (pilU) [Aeromonas sp. BC14]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCGCTGACAGAAGCCCCCCTCGACAAGGCAGGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCCTGAGCACTTCGAGCGCTATCTGCACACCAAGGAGGCCAACTACGCCATTCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGGCCCGCATTCCCACCTTTGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CTGGCAAATCCACCACCCAGGCGGCCATGATCGGTTATCGCAACCAGCATATGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCGCCAGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACAGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGGTGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.374

95.664

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.692

89.702

0.374
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36