Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NRG74_RS13905 Genome accession   NZ_CP102428
Coordinates   3009958..3010992 (-) Length   344 a.a.
NCBI ID   WP_007161666.1    Uniprot ID   -
Organism   Pseudomonas oryzihabitans strain YY7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3004958..3015992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG74_RS13885 (NRG74_13885) - 3005469..3006026 (+) 558 WP_017640983.1 C40 family peptidase -
  NRG74_RS13890 (NRG74_13890) - 3006159..3007370 (+) 1212 WP_017640984.1 putative DNA modification/repair radical SAM protein -
  NRG74_RS13895 (NRG74_13895) - 3007371..3008819 (+) 1449 WP_257704532.1 UdgX family uracil-DNA binding protein -
  NRG74_RS13900 (NRG74_13900) pilU 3008816..3009958 (-) 1143 WP_017640986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NRG74_RS13905 (NRG74_13905) pilT 3009958..3010992 (-) 1035 WP_007161666.1 type IV pilus twitching motility protein PilT Machinery gene
  NRG74_RS13910 (NRG74_13910) - 3011119..3011814 (+) 696 WP_257704535.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRG74_RS13915 (NRG74_13915) proC 3011824..3012642 (+) 819 WP_257704537.1 pyrroline-5-carboxylate reductase -
  NRG74_RS13920 (NRG74_13920) - 3012652..3013245 (+) 594 WP_064309244.1 YggT family protein -
  NRG74_RS13925 (NRG74_13925) - 3013363..3015321 (+) 1959 WP_257704539.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37941.73 Da        Isoelectric Point: 6.8363

>NTDB_id=716928 NRG74_RS13905 WP_007161666.1 3009958..3010992(-) (pilT) [Pseudomonas oryzihabitans strain YY7]
MDITELLAFSARQGASDLHLSAGLPPMIRVDGDVRRINLPAFEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGGLGMQTLDMCL
KALLAKGLISRDAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=716928 NRG74_RS13905 WP_007161666.1 3009958..3010992(-) (pilT) [Pseudomonas oryzihabitans strain YY7]
ATGGATATCACCGAATTGCTGGCCTTTTCCGCGCGGCAAGGCGCCTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGGCCTTCGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTGGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGCGCCGGCGCGGTGTTCCGGACCATTCCGTCCAAGGTGCTGAGCATGGA
AGAGCTGGGCATGGGCGAGGTGTTCAAGAAGATCACCGACGTCCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTCGCGGCCATGCTCGACTACCTCAACAACACCAAGTACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTCCACGAATCCAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCTGGGCTTTTCCGA
GGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGGCACCTGGTTTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGATCGGGTG
GTGGACGTCTTTCCGGCGGAAGAGAAGTCCATGGTGCGTTCCATGCTGTCCGAATCCCTGCAGGCGGTGGTCTCCCAGAC
CCTGCTCAAGAAGGTCGGTGGCGGCCGGGTGGCGGCCCACGAAATCATGATCGGTACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGTGGCGGCCTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGCGCTGCTGGCCAAGGGGCTGATCAGCCGCGACGCGGCCCGCGAGAAGGCCAAGACGCCGGAGAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.64

100

0.956
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter nosocomialis M2

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

82.558

100

0.826
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39