Detailed information NTG0716927

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NRG74_RS13900
• Genome accession: NZ_CP102428
• Coordinates: 3008816..3009958 (-)
• Length: 380 a.a.
• NCBI ID: WP_017640986.1
• Uniprot ID: A0A1H8VYP4
• Organism: Pseudomonas oryzihabitans strain YY7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3003816..3014958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NRG74_RS13880 (NRG74_13880)	-	3004630..3005034 (-)	405	WP_017640982.1	TM2 domain-containing protein	-
NRG74_RS13885 (NRG74_13885)	-	3005469..3006026 (+)	558	WP_017640983.1	C40 family peptidase	-
NRG74_RS13890 (NRG74_13890)	-	3006159..3007370 (+)	1212	WP_017640984.1	putative DNA modification/repair radical SAM protein	-
NRG74_RS13895 (NRG74_13895)	-	3007371..3008819 (+)	1449	WP_257704532.1	UdgX family uracil-DNA binding protein	-
NRG74_RS13900 (NRG74_13900)	pilU	3008816..3009958 (-)	1143	WP_017640986.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NRG74_RS13905 (NRG74_13905)	pilT	3009958..3010992 (-)	1035	WP_007161666.1	type IV pilus twitching motility protein PilT	Machinery gene
NRG74_RS13910 (NRG74_13910)	-	3011119..3011814 (+)	696	WP_257704535.1	YggS family pyridoxal phosphate-dependent enzyme	-
NRG74_RS13915 (NRG74_13915)	proC	3011824..3012642 (+)	819	WP_257704537.1	pyrroline-5-carboxylate reductase	-
NRG74_RS13920 (NRG74_13920)	-	3012652..3013245 (+)	594	WP_064309244.1	YggT family protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42412.72 Da
• Isoelectric Point: 7.3062

>NTDB_id=716927 NRG74_RS13900 WP_017640986.1 3008816..3009958(-) (pilU) [Pseudomonas oryzihabitans strain YY7]
MDFDRFLQLMVDKGASDLFITTGVPPSMKINGRMTPVTKDSLTPDQCRAIVLGVMNDEQRQEFQQKRECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIEVNIPTVEQLRLPEIIKKLAMTKRGLVIFVGATGTGKSTSLAAMIGHRNRNSAGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEIRSRDTMEHALAFAETGHLCLATLHANNANQALDRI
IHFFPADRHSQTWMDLSLNLKGIVAQQLVPTTDGQGRRAVIEVLLNTPLAADLIRKGEVHELKALMKKSGELGMQTFDQS
LYGLYRDGEISYEAALAHADSANDLRLMIKLGSETDADHLNQLTRNLSVDMGDDPPKRFR

Nucleotide

• Length: 1143 bp

>NTDB_id=716927 NRG74_RS13900 WP_017640986.1 3008816..3009958(-) (pilU) [Pseudomonas oryzihabitans strain YY7]
ATGGATTTCGACAGGTTCCTGCAGCTGATGGTGGACAAGGGGGCGTCCGACCTCTTCATCACCACCGGCGTGCCCCCTTC
GATGAAGATCAACGGGCGCATGACGCCGGTCACCAAGGATTCGCTCACGCCGGATCAGTGCCGAGCCATCGTGCTGGGGG
TGATGAACGACGAGCAGCGGCAGGAATTCCAGCAGAAGCGCGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGTCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGGTCAACATTCCCACGGTCGA
GCAGCTGCGCCTGCCGGAGATCATCAAGAAGCTGGCCATGACCAAGCGCGGGCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCCATGATCGGCCATCGCAACAGGAACTCGGCGGGGCACATCATCTCCATCGAGGAT
CCCATCGAGTACATCCACCAGCACCAGGGGTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAATCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCATGATCGGCGAGATCCGCAGCCGGGACACCATGGAGCACG
CCCTGGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCCATTTCTTTCCGGCCGATCGGCACAGCCAGACCTGGATGGACCTGTCGCTCAATCTCAAGGGCATCGTCGCCCAGCA
ACTGGTGCCCACCACCGATGGCCAGGGCCGTCGTGCCGTGATCGAGGTGCTGCTCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAACTCAAGGCGCTGATGAAGAAATCCGGCGAACTGGGCATGCAGACCTTCGACCAGTCG
CTCTATGGTCTCTATCGCGACGGCGAGATCAGCTACGAAGCGGCCTTGGCCCACGCCGATTCCGCCAACGACCTGCGGCT
GATGATCAAGCTGGGGTCGGAAACGGACGCCGATCACCTCAATCAGCTCACCCGCAACCTCTCGGTGGACATGGGCGACG
ATCCGCCCAAGCGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: A0A1H8VYP4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	77.165	100	0.774
pilU	Acinetobacter baylyi ADP1	62.766	98.947	0.621
pilU	Vibrio cholerae strain A1552	56.873	97.632	0.555
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.888	89.211	0.374