Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NR756_RS00455 Genome accession   NZ_CP102389
Coordinates   92574..93608 (-) Length   344 a.a.
NCBI ID   WP_022997480.1    Uniprot ID   A0A9Q3ZB68
Organism   Alloalcanivorax xenomutans strain AX     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 87574..98608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR756_RS00430 (NR756_00425) - 88336..88944 (+) 609 WP_022997476.1 FMN-dependent NADH-azoreductase -
  NR756_RS00435 (NR756_00430) - 88992..89996 (+) 1005 WP_416391468.1 glutathione S-transferase family protein -
  NR756_RS00440 (NR756_00435) - 90337..91191 (+) 855 WP_116027484.1 pirin family protein -
  NR756_RS00445 (NR756_00440) - 91175..91381 (-) 207 WP_143247737.1 hypothetical protein -
  NR756_RS00450 (NR756_00445) pilU 91434..92555 (-) 1122 WP_022997479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NR756_RS00455 (NR756_00450) pilT 92574..93608 (-) 1035 WP_022997480.1 type IV pilus twitching motility protein PilT Machinery gene
  NR756_RS00460 (NR756_00455) - 93727..94431 (+) 705 WP_080530121.1 YggS family pyridoxal phosphate-dependent enzyme -
  NR756_RS00465 (NR756_00460) proC 94464..95276 (+) 813 WP_063141404.1 pyrroline-5-carboxylate reductase -
  NR756_RS00470 (NR756_00465) - 95690..96010 (-) 321 WP_416391469.1 transposase -
  NR756_RS00475 (NR756_00470) - 96070..97014 (-) 945 WP_416390856.1 IS110 family transposase -
  NR756_RS00480 (NR756_00475) - 97470..98018 (+) 549 WP_022997221.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38028.65 Da        Isoelectric Point: 6.5061

>NTDB_id=716775 NR756_RS00455 WP_022997480.1 92574..93608(-) (pilT) [Alloalcanivorax xenomutans strain AX]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKEVHSLIYEIMNDKQRKDYEEFLETDFSFDVPSVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGKVFQNIASLPRGIVLVTGPTGSGKSTTLAAMMDYINNTRYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGASVGMQTLDQCL
QGLVQRGLVSREVAREKAKNPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=716775 NR756_RS00455 WP_022997480.1 92574..93608(-) (pilT) [Alloalcanivorax xenomutans strain AX]
ATGGATATCACCGAATTGCTCGCCTTCAGCGCCAAGAACGGCGCTTCGGATCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATTCGCGTGGACGGCGACGTGCGCCGCATCAATCTGCCGCCCATGGAGCACAAGGAAGTGCACTCGCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGACTACGAGGAGTTCCTGGAAACCGACTTCTCCTTCGATGTGCCCAGCGTGGCCCGT
TTCCGGGTCAACGCCTTCAATCAGAACCGGGGCGCCGGCGCCGTCTTCCGGACCATTCCCTCCAAGGTGCTGAGCATGGA
GGAACTGGGCATGGGCAAGGTGTTCCAGAACATCGCCTCGCTGCCGCGCGGCATCGTGTTGGTCACCGGCCCCACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGATGGACTACATCAATAACACCCGCTACGAACACATCCTCACCATCGAGGAC
CCGATTGAATTCGTCCATGAATCGAAGAAATGTCTGGTCAACCAGAGGGAAGTGCACCGGGACACCCATGGTTTCGCCGA
AGCCCTGCGCTCGGCACTGCGGGAGGACCCGGACATCATCCTGGTGGGGGAGATGCGGGATCTGGAGACCATCCGGCTGG
CACTGACCGCCGCCGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCAGCGCCGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGCCGAGGAGAAGGCCATGGTCCGCTCCATGCTTTCCGAATCCCTGCAAGCGGTGGTGTCGCAAAC
GCTGATGAAGAAAAACGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATTCGAAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGCCTCGGTGGGCATGCAGACTCTGGATCAATGCCTG
CAAGGGCTGGTGCAGCGCGGACTGGTATCCCGCGAAGTGGCCCGGGAAAAAGCCAAGAACCCGGACTCAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

86.919

100

0.869
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

81.105

100

0.811
  pilT Legionella pneumophila strain Lp02

75.588

98.837

0.747
  pilT Legionella pneumophila strain ERS1305867

75.588

98.837

0.747
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.339

99.419

0.52
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384