Detailed information NTG0716774

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NR756_RS00450
• Genome accession: NZ_CP102389
• Coordinates: 91434..92555 (-)
• Length: 373 a.a.
• NCBI ID: WP_022997479.1
• Uniprot ID: A0A9Q3ZEX2
• Organism: Alloalcanivorax xenomutans strain AX
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 86434..97555

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NR756_RS00420 (NR756_00415)	-	86764..87297 (-)	534	WP_022997474.1	DUF4136 domain-containing protein	-
NR756_RS00425 (NR756_00420)	-	87345..88238 (-)	894	WP_022997475.1	LysR family transcriptional regulator	-
NR756_RS00430 (NR756_00425)	-	88336..88944 (+)	609	WP_022997476.1	FMN-dependent NADH-azoreductase	-
NR756_RS00435 (NR756_00430)	-	88992..89996 (+)	1005	WP_416391468.1	glutathione S-transferase family protein	-
NR756_RS00440 (NR756_00435)	-	90337..91191 (+)	855	WP_116027484.1	pirin family protein	-
NR756_RS00445 (NR756_00440)	-	91175..91381 (-)	207	WP_143247737.1	hypothetical protein	-
NR756_RS00450 (NR756_00445)	pilU	91434..92555 (-)	1122	WP_022997479.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NR756_RS00455 (NR756_00450)	pilT	92574..93608 (-)	1035	WP_022997480.1	type IV pilus twitching motility protein PilT	Machinery gene
NR756_RS00460 (NR756_00455)	-	93727..94431 (+)	705	WP_080530121.1	YggS family pyridoxal phosphate-dependent enzyme	-
NR756_RS00465 (NR756_00460)	proC	94464..95276 (+)	813	WP_063141404.1	pyrroline-5-carboxylate reductase	-
NR756_RS00470 (NR756_00465)	-	95690..96010 (-)	321	WP_416391469.1	transposase	-
NR756_RS00475 (NR756_00470)	-	96070..97014 (-)	945	WP_416390856.1	IS110 family transposase	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41549.72 Da
• Isoelectric Point: 6.7399

>NTDB_id=716774 NR756_RS00450 WP_022997479.1 91434..92555(-) (pilU) [Alloalcanivorax xenomutans strain AX]
MEFEDLLKLMVQKGGSDLFITAGVPPSMKVHGKVVPVTRNALTPDMTRDLVFGVMSEKQRKEFVDQHECNFALSSRGIGR
FRVSAFYQRNLVGMVLRRIETKIPDADELRLPPIIKDLAMTKRGLVIFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
PIEFIHQHQGCIVTQREVGLDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLRGIVAQQLIPTPDGKGRRAAIEVLLNTPLVADHIRKGEVHLLKALMGKSREQGMQTFDQA
LYDLYDAGEITYEDAIAHADSPNDLRLMIKLANETDASSLDHATRGLSIEDSD

Nucleotide

• Length: 1122 bp

>NTDB_id=716774 NR756_RS00450 WP_022997479.1 91434..92555(-) (pilU) [Alloalcanivorax xenomutans strain AX]
ATGGAATTCGAAGATCTGCTCAAACTGATGGTGCAAAAAGGCGGCTCGGACTTGTTCATCACCGCCGGTGTTCCACCAAG
TATGAAAGTCCACGGCAAGGTGGTGCCGGTTACCCGTAACGCCCTGACCCCGGACATGACCCGGGATCTGGTGTTCGGTG
TGATGTCGGAAAAGCAGCGCAAGGAGTTCGTCGACCAGCACGAATGCAATTTCGCGCTATCCTCGCGGGGCATTGGCCGC
TTCCGTGTGTCCGCTTTTTACCAGCGCAATCTGGTGGGCATGGTGTTGCGGCGCATCGAGACCAAGATACCCGACGCCGA
CGAACTGCGGCTGCCGCCGATCATCAAGGACCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGGAAATCCACCTCCCTGGCGTCAATGATCGGCCACCGTAATCGCAACTCCAAAGGCCATATCATCTCCATTGAGGAT
CCGATCGAATTCATCCACCAGCATCAGGGCTGCATTGTCACGCAACGGGAAGTAGGCCTGGATACGGACAGTTTCGAGAT
CGCCCTCAAGAACACGCTGCGACAGGCACCGGATGTGATTCTGATCGGTGAGATCCGCAGCCGCGAAGTGATGGATTACG
CCATTGCCTTCGCCGAAACCGGCCATCTGTGTCTGGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATTCACTTTTTCCCCGCCGACCGTCACCGGCAGTTATGGATGGACCTCTCCCTGAACCTGCGCGGCATTGTCGCCCAGCA
ACTGATCCCGACGCCGGACGGCAAGGGCCGCCGCGCCGCCATCGAAGTGCTGCTGAACACGCCACTGGTGGCGGACCATA
TCCGTAAAGGCGAAGTGCATTTGCTCAAGGCGCTGATGGGCAAATCCCGGGAGCAGGGCATGCAGACTTTCGATCAGGCC
CTCTATGATCTTTATGATGCCGGCGAAATCACTTACGAGGACGCGATTGCCCACGCCGACTCCCCCAACGATCTGCGCCT
GATGATCAAGCTCGCCAACGAAACCGACGCCTCCAGCCTGGACCACGCCACCCGTGGCCTGAGCATCGAGGACTCGGACT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	76.944	100	0.769
pilU	Acinetobacter baylyi ADP1	67.385	99.464	0.67
pilU	Vibrio cholerae strain A1552	56.267	96.247	0.542
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.017	95.71	0.402