Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NR989_RS02600 Genome accession   NZ_CP102381
Coordinates   585946..586983 (-) Length   345 a.a.
NCBI ID   WP_275595414.1    Uniprot ID   -
Organism   Thiomicrorhabdus lithotrophica strain XGS-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 580946..591983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR989_RS02580 (NR989_02585) - 581744..582622 (+) 879 WP_275595410.1 hypothetical protein -
  NR989_RS02585 (NR989_02590) - 582609..583733 (+) 1125 WP_275595411.1 murein L,D-transpeptidase family protein -
  NR989_RS02590 (NR989_02595) - 583800..584765 (-) 966 WP_275595412.1 DUF2817 domain-containing protein -
  NR989_RS02595 (NR989_02600) pilU 584777..585934 (-) 1158 WP_275595413.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NR989_RS02600 (NR989_02605) pilT 585946..586983 (-) 1038 WP_275595414.1 type IV pilus twitching motility protein PilT Machinery gene
  NR989_RS02605 (NR989_02610) - 587155..587616 (-) 462 WP_275595415.1 DNA polymerase III subunit chi -
  NR989_RS02610 (NR989_02615) - 587625..589121 (-) 1497 WP_275595416.1 leucyl aminopeptidase -
  NR989_RS02615 (NR989_02620) lptF 589225..590319 (+) 1095 WP_275595417.1 LPS export ABC transporter permease LptF -
  NR989_RS02620 (NR989_02625) lptG 590321..591406 (+) 1086 WP_275595418.1 LPS export ABC transporter permease LptG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38537.17 Da        Isoelectric Point: 5.8992

>NTDB_id=716713 NR989_RS02600 WP_275595414.1 585946..586983(-) (pilT) [Thiomicrorhabdus lithotrophica strain XGS-01]
MDITQLLEFSVQQGASDLHLSTGQPPIIRKDGDVQRIEAPNLEAEQLQTMIYDIMNDEQRRGFESELESDFSFELPNIAR
FRANIFMHNRGIGAVFRTIPSKIITLEELECPEIFKDISSFPRGLVLVTGPTGSGKSTTLAAMVDYINKKEAAHILTVED
PIEFVHTPIRSLINQREVHRDTKGFTNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRSMLSESLRAVISQALLKKTTGGRIASHEIMLVNSAIRNLIREAKVPQMYSVIQTSKQLGMQTLDQNL
QDLVSKNVITREDARQKAVNKAAFA

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=716713 NR989_RS02600 WP_275595414.1 585946..586983(-) (pilT) [Thiomicrorhabdus lithotrophica strain XGS-01]
ATGGATATCACTCAATTATTAGAATTTAGCGTTCAGCAAGGTGCTTCAGATTTACACCTTTCTACTGGACAACCTCCAAT
TATCCGTAAAGATGGTGATGTTCAACGTATTGAAGCGCCTAATTTAGAAGCTGAACAGCTACAGACTATGATTTATGACA
TCATGAATGATGAGCAACGTCGTGGCTTTGAATCTGAACTAGAAAGCGATTTCTCTTTTGAACTACCCAACATTGCTCGA
TTCCGTGCAAATATTTTTATGCATAATCGGGGTATAGGCGCGGTTTTCCGTACTATTCCAAGTAAGATTATTACGCTTGA
AGAATTAGAATGTCCTGAAATTTTTAAAGATATTTCTAGCTTTCCGCGTGGACTAGTTCTTGTAACAGGCCCAACTGGTT
CTGGTAAATCTACTACGCTTGCTGCCATGGTGGATTACATTAATAAAAAAGAAGCTGCACACATTCTAACGGTTGAAGAT
CCTATCGAATTTGTTCACACCCCCATTCGCAGTTTAATCAACCAGCGTGAAGTTCACCGTGACACTAAAGGGTTTACCAA
TGCCCTTCGTTCCGCTTTACGTGAGGATCCAGACGTTATTTTAGTCGGTGAGATGCGAGATTTGGAAACCATTCGTCTTG
CTTTGACCGCTGCTGAAACAGGACATTTAGTTTTTGGAACATTGCATACGAGTTCTGCAGCAAAAACCATTGACCGTATT
ATTGATGTATTTCCAGCTGAAGAGAAAGAGATGGTTCGCTCAATGCTTTCGGAATCATTAAGAGCCGTTATTTCTCAAGC
ACTGTTAAAGAAAACCACTGGTGGTCGAATCGCTTCTCACGAGATCATGTTAGTTAACTCTGCTATTCGTAACCTAATCC
GTGAAGCGAAAGTACCGCAGATGTACTCGGTAATACAGACTTCGAAGCAATTAGGTATGCAGACATTAGATCAAAATTTA
CAAGATTTAGTCTCTAAAAACGTAATTACTCGTGAAGATGCTAGACAAAAAGCCGTTAATAAAGCGGCATTTGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.221

99.71

0.71
  pilT Acinetobacter baylyi ADP1

71.221

99.71

0.71
  pilT Pseudomonas aeruginosa PAK

72.485

97.971

0.71
  pilT Acinetobacter baumannii D1279779

70.93

99.71

0.707
  pilT Acinetobacter baumannii strain A118

70.93

99.71

0.707
  pilT Pseudomonas stutzeri DSM 10701

71.006

97.971

0.696
  pilT Legionella pneumophila strain Lp02

66.279

99.71

0.661
  pilT Legionella pneumophila strain ERS1305867

66.279

99.71

0.661
  pilT Neisseria meningitidis 8013

64.74

100

0.649
  pilT Neisseria gonorrhoeae MS11

64.451

100

0.646
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.681

0.646
  pilT Vibrio cholerae strain A1552

66.172

97.681

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.867

100

0.51
  pilU Vibrio cholerae strain A1552

40.118

98.261

0.394
  pilU Pseudomonas stutzeri DSM 10701

40

97.101

0.388
  pilU Acinetobacter baylyi ADP1

37.353

98.551

0.368