Detailed information NTG0716712

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NR989_RS02595
• Genome accession: NZ_CP102381
• Coordinates: 584777..585934 (-)
• Length: 385 a.a.
• NCBI ID: WP_275595413.1
• Uniprot ID: -
• Organism: Thiomicrorhabdus lithotrophica strain XGS-01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 579777..590934

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NR989_RS02580 (NR989_02585)	-	581744..582622 (+)	879	WP_275595410.1	hypothetical protein	-
NR989_RS02585 (NR989_02590)	-	582609..583733 (+)	1125	WP_275595411.1	murein L,D-transpeptidase family protein	-
NR989_RS02590 (NR989_02595)	-	583800..584765 (-)	966	WP_275595412.1	DUF2817 domain-containing protein	-
NR989_RS02595 (NR989_02600)	pilU	584777..585934 (-)	1158	WP_275595413.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NR989_RS02600 (NR989_02605)	pilT	585946..586983 (-)	1038	WP_275595414.1	type IV pilus twitching motility protein PilT	Machinery gene
NR989_RS02605 (NR989_02610)	-	587155..587616 (-)	462	WP_275595415.1	DNA polymerase III subunit chi	-
NR989_RS02610 (NR989_02615)	-	587625..589121 (-)	1497	WP_275595416.1	leucyl aminopeptidase	-
NR989_RS02615 (NR989_02620)	lptF	589225..590319 (+)	1095	WP_275595417.1	LPS export ABC transporter permease LptF	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42668.68 Da
• Isoelectric Point: 5.5923

>NTDB_id=716712 NR989_RS02595 WP_275595413.1 584777..585934(-) (pilU) [Thiomicrorhabdus lithotrophica strain XGS-01]
MVVDTESLSELDKLLVFFSNNGGSDLFITAGRAATMKKDGKLVSLTEDKLTADTATKLAYQMMNHDQLATFQNTQEINFA
YHISGIARYRVNIFIQRGTPGMVIRSVHTEIPTFEDLNLPSIIKETITDKNGLILIVGGTGSGKSTTLASLVDVRNTTMD
GHIITIEDPIEFIHRHKKSIVTQREVGVDTESYESALKNTLRQAPDVILIGEIRSRDTMEHAIAFSETGHLCLSTLHANN
TNQTLDRIINFFPANQRGHILMDLSLNLKAVISQRLIPKIGGGLIAAVEVLINTPRIAELIFHGKVNEIKQLMQNSSALG
MQTFDQALFDLYENGLITYQDALRGADSMNDLRLNIKLNSKLPQPSDDDSELSTEAIKFSLKQEE

Nucleotide

• Length: 1158 bp

>NTDB_id=716712 NR989_RS02595 WP_275595413.1 584777..585934(-) (pilU) [Thiomicrorhabdus lithotrophica strain XGS-01]
ATGGTAGTTGATACAGAAAGCCTGTCAGAGTTAGATAAATTATTAGTCTTCTTTTCCAACAACGGAGGTTCTGATTTATT
CATTACTGCTGGTCGTGCTGCAACCATGAAAAAAGATGGCAAGTTAGTCTCCCTTACGGAAGATAAACTGACTGCGGACA
CAGCAACAAAGCTTGCCTATCAAATGATGAACCACGACCAATTAGCTACCTTTCAGAATACTCAAGAGATTAACTTCGCC
TATCATATCAGTGGTATTGCACGTTACCGTGTTAACATTTTTATTCAACGTGGTACACCTGGAATGGTAATACGTTCAGT
GCATACAGAGATACCGACGTTTGAAGACTTAAACCTACCTAGCATTATTAAAGAGACTATCACAGATAAGAATGGTTTAA
TTTTGATTGTAGGTGGAACAGGTTCAGGTAAATCAACAACACTAGCAAGCTTAGTGGATGTAAGAAATACAACAATGGAT
GGTCATATCATTACTATTGAGGACCCTATTGAGTTTATCCACCGTCATAAAAAGAGTATCGTAACTCAACGAGAAGTAGG
CGTTGATACTGAAAGCTATGAAAGCGCACTTAAAAACACCTTACGACAAGCACCCGATGTCATATTAATAGGTGAAATTC
GAAGCCGAGATACAATGGAGCATGCCATTGCCTTCTCAGAAACAGGTCACTTATGCCTTTCTACTTTACATGCTAACAAT
ACTAACCAAACACTTGACCGAATTATAAACTTTTTTCCGGCGAATCAACGCGGACACATTTTAATGGATTTATCACTTAA
CTTAAAAGCGGTTATCTCACAACGACTCATTCCCAAAATAGGCGGAGGTTTAATTGCTGCAGTAGAAGTTTTGATCAACA
CCCCTCGTATTGCTGAGCTTATATTTCATGGAAAGGTAAATGAAATCAAACAGTTAATGCAAAATTCAAGTGCTTTAGGA
ATGCAGACTTTTGACCAGGCATTATTTGACTTGTATGAAAATGGATTAATCACTTACCAAGACGCCCTAAGGGGTGCAGA
CTCTATGAATGATTTACGTTTAAATATAAAGTTAAATAGTAAATTACCGCAGCCTAGCGATGACGATAGCGAACTTTCAA
CAGAAGCGATTAAATTTAGTTTAAAGCAAGAAGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (24-278)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.507	91.688	0.527
pilU	Acinetobacter baylyi ADP1	54.372	95.065	0.517
pilU	Vibrio cholerae strain A1552	53.868	90.649	0.488
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.012	87.792	0.369