Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NRZ28_RS03365 Genome accession   NZ_CP102366
Coordinates   713519..714553 (-) Length   344 a.a.
NCBI ID   WP_005305010.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BC16     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708519..719553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ28_RS03345 (NRZ28_03345) - 709337..710047 (-) 711 WP_268032150.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NRZ28_RS03350 (NRZ28_03350) srmB 710176..711399 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  NRZ28_RS03355 (NRZ28_03355) yaaA 711576..712349 (-) 774 WP_187268867.1 peroxide stress protein YaaA -
  NRZ28_RS03360 (NRZ28_03360) pilU 712381..713490 (-) 1110 WP_187268866.1 type IVa pilus ATPase TapU Machinery gene
  NRZ28_RS03365 (NRZ28_03365) pilT 713519..714553 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NRZ28_RS03370 (NRZ28_03370) - 714593..715294 (+) 702 WP_016351971.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRZ28_RS03375 (NRZ28_03375) proC 715353..716177 (+) 825 WP_268032151.1 pyrroline-5-carboxylate reductase -
  NRZ28_RS03380 (NRZ28_03380) - 716218..716769 (+) 552 WP_017411214.1 YggT family protein -
  NRZ28_RS03385 (NRZ28_03385) yggU 716769..717068 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  NRZ28_RS03390 (NRZ28_03390) - 717087..717506 (+) 420 WP_060390705.1 DUF4426 domain-containing protein -
  NRZ28_RS03395 (NRZ28_03395) - 717563..717874 (-) 312 WP_268032152.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38319.12 Da        Isoelectric Point: 6.7439

>NTDB_id=716497 NRZ28_RS03365 WP_005305010.1 713519..714553(-) (pilT) [Aeromonas hydrophila strain BC16]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=716497 NRZ28_RS03365 WP_005305010.1 713519..714553(-) (pilT) [Aeromonas hydrophila strain BC16]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAGGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCGAACCTCGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCACGCGGCTCCGGCGCGGTATTTCGGACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTGGACGCCCCGGAGATCTTTCGCAAGATTGCCGAATACCCCCGCGGTCTGGTACTGGTGACCGGCCCGACCGGCT
CGGGCAAATCCACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTGGAAACCATTCGTCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAGTCGCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGCGGCGGCCGGGTGGCGGCCCACGAAATCATGATGGGCATTCCCGCCGTACGAAACCTCATCC
GGGAAGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGCGGCATGGTGGCGGCGCTCGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407