Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRZ28_RS03360 Genome accession   NZ_CP102366
Coordinates   712381..713490 (-) Length   369 a.a.
NCBI ID   WP_187268866.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BC16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707381..718490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ28_RS03340 (NRZ28_03340) brnQ 707838..709127 (-) 1290 WP_187268869.1 branched-chain amino acid transport system II carrier protein -
  NRZ28_RS03345 (NRZ28_03345) - 709337..710047 (-) 711 WP_268032150.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NRZ28_RS03350 (NRZ28_03350) srmB 710176..711399 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  NRZ28_RS03355 (NRZ28_03355) yaaA 711576..712349 (-) 774 WP_187268867.1 peroxide stress protein YaaA -
  NRZ28_RS03360 (NRZ28_03360) pilU 712381..713490 (-) 1110 WP_187268866.1 type IVa pilus ATPase TapU Machinery gene
  NRZ28_RS03365 (NRZ28_03365) pilT 713519..714553 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NRZ28_RS03370 (NRZ28_03370) - 714593..715294 (+) 702 WP_016351971.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRZ28_RS03375 (NRZ28_03375) proC 715353..716177 (+) 825 WP_268032151.1 pyrroline-5-carboxylate reductase -
  NRZ28_RS03380 (NRZ28_03380) - 716218..716769 (+) 552 WP_017411214.1 YggT family protein -
  NRZ28_RS03385 (NRZ28_03385) yggU 716769..717068 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  NRZ28_RS03390 (NRZ28_03390) - 717087..717506 (+) 420 WP_060390705.1 DUF4426 domain-containing protein -
  NRZ28_RS03395 (NRZ28_03395) - 717563..717874 (-) 312 WP_268032152.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40935.04 Da        Isoelectric Point: 6.3880

>NTDB_id=716496 NRZ28_RS03360 WP_187268866.1 712381..713490(-) (pilU) [Aeromonas hydrophila strain BC16]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLTEAPLDKAGALALVKETLSPEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHMDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEVHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=716496 NRZ28_RS03360 WP_187268866.1 712381..713490(-) (pilU) [Aeromonas hydrophila strain BC16]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCGCTGACAGAAGCCCCCCTCGACAAGGCAGGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCCTGAGCACTTCGAGCGCTATCTGCACACCAAGGAGGCCAACTACGCCATTCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATTCCCACCTTTGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGTTATCGCAACCAGCATATGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACTCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCGCCAGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAAACAGGTCATCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGATGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGGTGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363