Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DLM_RS21890 Genome accession   NZ_AP018823
Coordinates   4653119..4653547 (+) Length   142 a.a.
NCBI ID   WP_089083022.1    Uniprot ID   -
Organism   Aquitalea magnusonii strain H3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4648119..4658547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLM_RS21870 (DLM_4359) proB 4648273..4649391 (-) 1119 WP_089082808.1 glutamate 5-kinase -
  DLM_RS21875 (DLM_4360) - 4649468..4651006 (-) 1539 WP_089082807.1 hypothetical protein -
  DLM_RS21880 (DLM_4361) - 4651131..4652480 (-) 1350 WP_089082806.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  DLM_RS23930 pilA2 4652669..4653097 (+) 429 WP_089082805.1 pilin Machinery gene
  DLM_RS21890 pilA 4653119..4653547 (+) 429 WP_089083022.1 pilin Machinery gene
  DLM_RS21895 (DLM_4362) - 4653606..4655432 (+) 1827 WP_089082804.1 PglL family O-oligosaccharyltransferase -
  DLM_RS21900 (DLM_4363) - 4655527..4657314 (+) 1788 WP_089082803.1 PglL family O-oligosaccharyltransferase -
  DLM_RS21905 (DLM_4364) rpoH 4657429..4658292 (-) 864 WP_089082802.1 RNA polymerase sigma factor RpoH -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14577.49 Da        Isoelectric Point: 7.7778

>NTDB_id=71551 DLM_RS21890 WP_089083022.1 4653119..4653547(+) (pilA) [Aquitalea magnusonii strain H3]
MNSKIQQGFTLIELMIVVAIIGILAAIAIPAYQDYTKRARVTEGLSLASAAKTAVAEYYASNNTFPSTNASAGLADTIQG
NAVSSVSLSGSGVITVTYNSLVDTASNTLTLTPAASNGAITWKCAAGTIATNWLPSNCRNGN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=71551 DLM_RS21890 WP_089083022.1 4653119..4653547(+) (pilA) [Aquitalea magnusonii strain H3]
ATGAACAGCAAGATCCAGCAAGGTTTTACCCTGATCGAACTGATGATTGTCGTAGCCATTATCGGTATTCTGGCTGCCAT
CGCCATCCCGGCCTACCAGGATTACACCAAGCGTGCGCGTGTGACAGAAGGTCTGTCGTTGGCTTCTGCGGCCAAAACGG
CTGTTGCCGAGTACTATGCATCTAATAATACATTCCCCTCAACAAATGCCTCCGCCGGGTTGGCAGATACAATTCAGGGT
AATGCAGTTTCCAGTGTTTCGCTTAGTGGCTCGGGTGTGATTACCGTGACTTATAACTCTTTGGTCGATACGGCTAGCAA
TACTTTGACCTTGACTCCTGCTGCTTCTAATGGCGCAATTACTTGGAAGTGTGCCGCTGGAACTATTGCAACCAACTGGT
TGCCTTCCAATTGCCGTAATGGTAACTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

43.976

100

0.514
  pilA2 Legionella pneumophila str. Paris

53.333

95.07

0.507
  pilA2 Legionella pneumophila strain ERS1305867

53.333

95.07

0.507
  pilA/pilA1 Eikenella corrodens VA1

44.654

100

0.5
  comP Acinetobacter baylyi ADP1

48.276

100

0.493
  pilE Neisseria gonorrhoeae strain FA1090

42.236

100

0.479
  pilE Neisseria gonorrhoeae MS11

41.975

100

0.479
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.054

100

0.444
  pilA Vibrio cholerae C6706

40

100

0.423
  pilA Vibrio cholerae strain A1552

40

100

0.423
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40

100

0.423
  pilA Haemophilus influenzae Rd KW20

42.446

97.887

0.415
  pilA Acinetobacter baumannii strain A118

40.426

99.296

0.401
  pilA Haemophilus influenzae 86-028NP

41.606

96.479

0.401
  pilA Vibrio parahaemolyticus RIMD 2210633

42.857

88.732

0.38
  pilA Pseudomonas aeruginosa PAK

35.333

100

0.373

Multiple sequence alignment