Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DLM_RS20160 Genome accession   NZ_AP018823
Coordinates   4288237..4289274 (-) Length   345 a.a.
NCBI ID   WP_089082489.1    Uniprot ID   A0A3G9GP98
Organism   Aquitalea magnusonii strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4283237..4294274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLM_RS20145 (DLM_4019) - 4284133..4285344 (+) 1212 WP_089082487.1 EAL and HDOD domain-containing protein -
  DLM_RS20150 (DLM_4020) - 4285381..4287006 (-) 1626 WP_089082488.1 response regulator -
  DLM_RS20155 (DLM_4021) pilU 4287063..4288199 (-) 1137 WP_089082521.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DLM_RS20160 (DLM_4022) pilT 4288237..4289274 (-) 1038 WP_089082489.1 type IV pilus twitching motility protein PilT Machinery gene
  DLM_RS20165 (DLM_4023) - 4289434..4290129 (+) 696 WP_089082490.1 YggS family pyridoxal phosphate-dependent enzyme -
  DLM_RS20170 (DLM_4024) proC 4290194..4290997 (+) 804 WP_089082491.1 pyrroline-5-carboxylate reductase -
  DLM_RS20175 (DLM_4025) - 4291004..4291585 (+) 582 WP_089082492.1 YggT family protein -
  DLM_RS20180 (DLM_4026) - 4291660..4291965 (+) 306 WP_089082493.1 c-type cytochrome -
  DLM_RS20185 (DLM_4027) dksA 4292167..4292577 (+) 411 WP_089082494.1 RNA polymerase-binding protein DksA -
  DLM_RS20190 (DLM_4028) aroG 4292738..4293814 (+) 1077 WP_089082495.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38414.05 Da        Isoelectric Point: 6.5772

>NTDB_id=71547 DLM_RS20160 WP_089082489.1 4288237..4289274(-) (pilT) [Aquitalea magnusonii strain H3]
MEISELLAFTVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLDHHDVHDMVYDIMNDYQRKIFEDEFECDFSFELPGVAR
FRVNAFVQNRGMGAVFRVIPSKVLTLEQLGAPKIFQEIASYPRGLVLVTGPTGSGKSTTLAAMVDFVNENNYSHILTVED
PIEFVHESKKSLINQRELGLQTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVISQTLLKTKDGNGRVAAHEIMIGTPAIRNLIRENKIAQINSMIQTGQQYGMQTLDQC
LQELVRRNIVAPNEARQKAASKDAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=71547 DLM_RS20160 WP_089082489.1 4288237..4289274(-) (pilT) [Aquitalea magnusonii strain H3]
ATGGAAATTTCCGAGCTCTTGGCCTTTACCGTCAAGAACAAGGCATCGGACCTGCACCTGTCGGCCGGTTTGCCTCCGAT
GATCCGGGTACACGGGGATGTGCGTCGCATCAATCTGCCGCCGCTGGACCATCATGATGTACACGATATGGTGTACGACA
TCATGAACGACTATCAACGCAAGATTTTCGAAGACGAATTCGAGTGCGACTTTTCCTTCGAACTGCCCGGCGTCGCCCGT
TTCCGGGTGAATGCCTTCGTGCAGAACCGCGGCATGGGTGCGGTATTCCGCGTGATTCCGTCCAAGGTGCTGACGCTGGA
GCAACTGGGTGCGCCCAAGATCTTCCAGGAGATCGCCAGCTACCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CCGGCAAATCCACCACGCTGGCAGCCATGGTGGATTTCGTCAACGAGAACAATTATTCGCACATCCTGACGGTGGAAGAT
CCGATCGAGTTCGTGCATGAAAGCAAAAAGTCGCTGATCAACCAGCGCGAGCTGGGCTTGCAGACTCACAGTTTCTCCAA
TGCCCTGCGCTCGGCCTTGCGTGAAGACCCGGACGTGATTCTGGTGGGCGAGTTGCGCGATCTGGAAACCATCCGGCTGG
CGCTGACAGCAGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCAGTTCGGCGGCAAAAACCATCGACCGTATC
GTGGACGTGTTCCCGGCGGGTGAAAAGGAAATGGTGCGCTCCATGCTGTCCGAATCGGTGCGGGCGGTGATTTCGCAAAC
CCTGCTCAAGACCAAGGACGGCAATGGCCGGGTGGCGGCGCATGAGATCATGATCGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAAACAAGATTGCCCAGATCAACTCGATGATCCAGACCGGCCAGCAATATGGCATGCAGACGCTGGATCAGTGC
CTGCAGGAACTGGTGCGGCGCAATATCGTGGCCCCTAACGAGGCCAGACAGAAAGCAGCGTCCAAGGATGCGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G9GP98

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

74.493

100

0.745
  pilT Acinetobacter baumannii strain A118

74.493

100

0.745
  pilT Acinetobacter baumannii D1279779

74.493

100

0.745
  pilT Pseudomonas aeruginosa PAK

73.623

100

0.736
  pilT Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilT Acinetobacter baylyi ADP1

72.464

100

0.725
  pilT Neisseria meningitidis 8013

72.464

100

0.725
  pilT Neisseria gonorrhoeae MS11

72.174

100

0.722
  pilT Legionella pneumophila strain Lp02

70.435

100

0.704
  pilT Legionella pneumophila strain ERS1305867

70.435

100

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.971

0.667
  pilT Vibrio cholerae strain A1552

68.047

97.971

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

40.816

99.42

0.406
  pilU Acinetobacter baylyi ADP1

39.766

99.13

0.394
  pilU Pseudomonas stutzeri DSM 10701

41.358

93.913

0.388

Multiple sequence alignment