Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DLM_RS20155 Genome accession   NZ_AP018823
Coordinates   4287063..4288199 (-) Length   378 a.a.
NCBI ID   WP_089082521.1    Uniprot ID   -
Organism   Aquitalea magnusonii strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4282063..4293199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLM_RS20135 (DLM_4017) - 4282468..4283139 (+) 672 WP_089082486.1 ABC transporter permease subunit -
  DLM_RS20140 (DLM_4018) - 4283145..4283879 (+) 735 WP_089082520.1 amino acid ABC transporter ATP-binding protein -
  DLM_RS20145 (DLM_4019) - 4284133..4285344 (+) 1212 WP_089082487.1 EAL and HDOD domain-containing protein -
  DLM_RS20150 (DLM_4020) - 4285381..4287006 (-) 1626 WP_089082488.1 response regulator -
  DLM_RS20155 (DLM_4021) pilU 4287063..4288199 (-) 1137 WP_089082521.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DLM_RS20160 (DLM_4022) pilT 4288237..4289274 (-) 1038 WP_089082489.1 type IV pilus twitching motility protein PilT Machinery gene
  DLM_RS20165 (DLM_4023) - 4289434..4290129 (+) 696 WP_089082490.1 YggS family pyridoxal phosphate-dependent enzyme -
  DLM_RS20170 (DLM_4024) proC 4290194..4290997 (+) 804 WP_089082491.1 pyrroline-5-carboxylate reductase -
  DLM_RS20175 (DLM_4025) - 4291004..4291585 (+) 582 WP_089082492.1 YggT family protein -
  DLM_RS20180 (DLM_4026) - 4291660..4291965 (+) 306 WP_089082493.1 c-type cytochrome -
  DLM_RS20185 (DLM_4027) dksA 4292167..4292577 (+) 411 WP_089082494.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42385.54 Da        Isoelectric Point: 6.1381

>NTDB_id=71546 DLM_RS20155 WP_089082521.1 4287063..4288199(-) (pilU) [Aquitalea magnusonii strain H3]
MEKDQASKFIHDLLKHAVGKNASDIFISAEFPPAMKIDGKITPVAPQPLSAQHTKELVRSVMNDRQTEEFESRKEANFAI
NPPGIGRFRVSAFIQQGCAGMVLRKINTEIPTFDKLNLPEVLQDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSHNQD
HIITIEDPIEYVHQHKKCIVTQREVGVDTEDWEIALKNTLRQAPDVILMGEIRDRETMGYALQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRSIISQRLLPIKSGRGRVAAVEILLNTPLISDMIFKGEIASLKEVMGRSRESG
MQTFDQSLFDLFEAGYISYEDALRNADSINDLRLKIKLYSESSKGRDPLDGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=71546 DLM_RS20155 WP_089082521.1 4287063..4288199(-) (pilU) [Aquitalea magnusonii strain H3]
ATGGAAAAAGATCAGGCATCAAAATTCATTCACGACCTGCTCAAGCATGCGGTCGGTAAAAACGCGTCCGACATCTTCAT
TTCCGCCGAATTTCCGCCGGCCATGAAGATAGACGGCAAGATCACGCCGGTGGCACCACAGCCCTTGTCGGCGCAGCACA
CCAAGGAGCTGGTGCGCTCGGTGATGAACGACCGGCAGACCGAAGAGTTCGAATCCAGGAAAGAAGCCAACTTTGCCATC
AACCCGCCGGGCATCGGACGCTTCCGGGTCAGTGCCTTCATCCAGCAAGGCTGTGCCGGCATGGTGCTGCGCAAGATCAA
TACCGAAATTCCCACCTTCGACAAGCTGAACCTGCCCGAGGTGCTTCAGGACATCGCGCTGATCAAGCGCGGGCTGGTGA
TTTTCGTGGGTGGCACCGGCTCGGGTAAATCCACCTCGCTGGCCGCACTGGTTGACTGGCGCAACAGCCATAACCAGGAC
CACATCATCACCATCGAGGACCCGATCGAATACGTGCATCAGCACAAGAAATGCATCGTGACCCAGCGCGAGGTGGGGGT
GGATACCGAAGACTGGGAAATCGCGCTGAAAAACACCCTGCGTCAGGCACCGGACGTGATCCTGATGGGGGAAATCCGCG
ATCGGGAAACCATGGGCTATGCACTGCAGTTTGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAATGCC
AACCAGGCGCTGGACCGCATTCTCAACTTCTTCCCGGAAGAGCGCCACCAGCAGGTGCTGATGGACCTGTCGCTGAACAT
GCGCTCCATCATTTCGCAGCGCCTGCTGCCGATCAAGAGCGGACGCGGGCGGGTGGCCGCAGTGGAAATCCTGCTTAACA
CCCCGCTGATTTCCGACATGATCTTCAAAGGTGAGATTGCCAGCCTGAAGGAGGTGATGGGCCGTTCGCGCGAGTCCGGC
ATGCAAACCTTCGACCAGTCGCTGTTCGACCTGTTTGAAGCCGGTTATATCAGTTACGAAGACGCACTGCGCAATGCCGA
TTCCATCAACGACCTGCGCCTGAAAATCAAGCTCTACAGCGAAAGCTCGAAAGGCCGTGACCCGCTGGATGGCATCGACC
ATCTGGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.571

92.593

0.579
  pilU Acinetobacter baylyi ADP1

57.895

95.503

0.553
  pilU Vibrio cholerae strain A1552

53.005

96.825

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

42.183

89.683

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.943

88.095

0.378
  pilT Vibrio cholerae strain A1552

42.943

88.095

0.378
  pilT Acinetobacter baumannii D1279779

42.643

88.095

0.376
  pilT Acinetobacter nosocomialis M2

42.643

88.095

0.376
  pilT Acinetobacter baumannii strain A118

42.643

88.095

0.376
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Neisseria meningitidis 8013

41.471

89.947

0.373
  pilT Legionella pneumophila strain Lp02

42.342

88.095

0.373
  pilT Legionella pneumophila strain ERS1305867

42.342

88.095

0.373
  pilT Neisseria gonorrhoeae MS11

40.95

89.153

0.365

Multiple sequence alignment