Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQU90_RS18690 Genome accession   NZ_CP102108
Coordinates   4060423..4061457 (-) Length   344 a.a.
NCBI ID   WP_123173807.1    Uniprot ID   A0AAN1UR77
Organism   Aeromonas veronii strain GD21SC2322TT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4055423..4066457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQU90_RS18660 (NQU90_18660) glnK 4056011..4056349 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  NQU90_RS18665 (NQU90_18665) arfB 4056551..4056967 (+) 417 WP_111901080.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  NQU90_RS18670 (NQU90_18670) - 4056993..4057484 (+) 492 WP_139747064.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  NQU90_RS18675 (NQU90_18675) - 4057844..4058269 (-) 426 WP_005340360.1 hypothetical protein -
  NQU90_RS18680 (NQU90_18680) yaaA 4058464..4059237 (-) 774 WP_005340358.1 peroxide stress protein YaaA -
  NQU90_RS18685 (NQU90_18685) pilU 4059285..4060394 (-) 1110 WP_257125024.1 type IVa pilus ATPase TapU Machinery gene
  NQU90_RS18690 (NQU90_18690) pilT 4060423..4061457 (-) 1035 WP_123173807.1 type IVa pilus ATPase TapT Machinery gene
  NQU90_RS18695 (NQU90_18695) - 4061497..4062198 (+) 702 WP_076495788.1 YggS family pyridoxal phosphate-dependent enzyme -
  NQU90_RS18700 (NQU90_18700) proC 4062341..4063165 (+) 825 WP_257123780.1 pyrroline-5-carboxylate reductase -
  NQU90_RS18705 (NQU90_18705) - 4063177..4063728 (+) 552 WP_047436998.1 YggT family protein -
  NQU90_RS18710 (NQU90_18710) yggU 4063728..4064030 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  NQU90_RS18715 (NQU90_18715) - 4064074..4064493 (+) 420 WP_257123781.1 DUF4426 domain-containing protein -
  NQU90_RS18720 (NQU90_18720) - 4064595..4065791 (-) 1197 WP_257123782.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38259.00 Da        Isoelectric Point: 6.7440

>NTDB_id=714465 NQU90_RS18690 WP_123173807.1 4060423..4061457(-) (pilT) [Aeromonas veronii strain GD21SC2322TT]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHGLIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=714465 NQU90_RS18690 WP_123173807.1 4060423..4061457(-) (pilT) [Aeromonas veronii strain GD21SC2322TT]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTTTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGGCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTACCCAATCTGGCCCGT
TTCCGGGTTAACGCCTTCCAGCAGGCGCGCGGTTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAAGAC
CCCATCGAATTTGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
TGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGTATC
ATCGACGTCTTCCCCGGTGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGTCGGGTGGCGGCTCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTGATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

75.872

100

0.759
  pilT Pseudomonas aeruginosa PAK

75

100

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.261

100

0.419
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407