Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NQU90_RS18685 Genome accession   NZ_CP102108
Coordinates   4059285..4060394 (-) Length   369 a.a.
NCBI ID   WP_257125024.1    Uniprot ID   -
Organism   Aeromonas veronii strain GD21SC2322TT     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4054285..4065394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQU90_RS18655 (NQU90_18655) - 4054745..4055752 (-) 1008 WP_257125023.1 Fe(3+) ABC transporter substrate-binding protein -
  NQU90_RS18660 (NQU90_18660) glnK 4056011..4056349 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  NQU90_RS18665 (NQU90_18665) arfB 4056551..4056967 (+) 417 WP_111901080.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  NQU90_RS18670 (NQU90_18670) - 4056993..4057484 (+) 492 WP_139747064.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  NQU90_RS18675 (NQU90_18675) - 4057844..4058269 (-) 426 WP_005340360.1 hypothetical protein -
  NQU90_RS18680 (NQU90_18680) yaaA 4058464..4059237 (-) 774 WP_005340358.1 peroxide stress protein YaaA -
  NQU90_RS18685 (NQU90_18685) pilU 4059285..4060394 (-) 1110 WP_257125024.1 type IVa pilus ATPase TapU Machinery gene
  NQU90_RS18690 (NQU90_18690) pilT 4060423..4061457 (-) 1035 WP_123173807.1 type IVa pilus ATPase TapT Machinery gene
  NQU90_RS18695 (NQU90_18695) - 4061497..4062198 (+) 702 WP_076495788.1 YggS family pyridoxal phosphate-dependent enzyme -
  NQU90_RS18700 (NQU90_18700) proC 4062341..4063165 (+) 825 WP_257123780.1 pyrroline-5-carboxylate reductase -
  NQU90_RS18705 (NQU90_18705) - 4063177..4063728 (+) 552 WP_047436998.1 YggT family protein -
  NQU90_RS18710 (NQU90_18710) yggU 4063728..4064030 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  NQU90_RS18715 (NQU90_18715) - 4064074..4064493 (+) 420 WP_257123781.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41106.14 Da        Isoelectric Point: 6.5043

>NTDB_id=714464 NQU90_RS18685 WP_257125024.1 4059285..4060394(-) (pilU) [Aeromonas veronii strain GD21SC2322TT]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKTIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=714464 NQU90_RS18685 WP_257125024.1 4059285..4060394(-) (pilU) [Aeromonas veronii strain GD21SC2322TT]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCTCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACGACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTAAAAAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGAAGTCAGGAGACCATGGAATTTG
CCCTGCAGTTCGCCGAGACAGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTTGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGACCATCGTCGCCCAGCA
GCTGGTGCCAAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAGTCCACCGAGCTGGGTATGCAGACCTTTGATCAGGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCTCTCGCCCACGCCGACTCGGCCAATGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.528

91.87

0.363
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36