NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	NQU59_RS04985	Genome accession	NZ_CP102099
Coordinates	1049724..1050143 (+)	Length	139 a.a.
NCBI ID	WP_373463000.1	Uniprot ID	-
Organism	Acinetobacter colistiniresistens strain C-214
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1051063..1059705	1049724..1050143	flank	920

Gene organization within MGE regions

Location: 1049724..1059705

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NQU59_RS04985 (NQU59_04985)	pilA	1049724..1050143 (+)	420	WP_373463000.1	pilin	Machinery gene
NQU59_RS04990 (NQU59_04990)	-	1050213..1050530 (+)	318	WP_257065245.1	hypothetical protein	-
NQU59_RS04995 (NQU59_04995)	-	1051063..1052151 (+)	1089	WP_257065246.1	M48 family metallopeptidase	-
NQU59_RS05000 (NQU59_05000)	-	1052148..1052999 (+)	852	WP_257065247.1	glycosyltransferase family 2 protein	-
NQU59_RS05005 (NQU59_05005)	-	1053008..1053358 (+)	351	WP_257065248.1	hypothetical protein	-
NQU59_RS05010 (NQU59_05010)	-	1053781..1054029 (+)	249	WP_257065249.1	hypothetical protein	-
NQU59_RS05015 (NQU59_05015)	-	1054201..1054695 (+)	495	WP_257065250.1	ATP-binding cassette domain-containing protein	-
NQU59_RS05020 (NQU59_05020)	-	1054742..1055227 (+)	486	WP_257065251.1	hypothetical protein	-
NQU59_RS05025 (NQU59_05025)	-	1055233..1055574 (+)	342	WP_257065252.1	hypothetical protein	-
NQU59_RS05030 (NQU59_05030)	-	1055567..1056157 (-)	591	Protein_956	TylF/MycF/NovP-related O-methyltransferase	-
NQU59_RS18835	-	1056367..1056729 (-)	363	WP_373463001.1	glycosyltransferase	-
NQU59_RS18840	-	1056794..1057246 (-)	453	WP_373463002.1	glycosyltransferase	-
NQU59_RS05035 (NQU59_05035)	-	1057874..1058149 (-)	276	WP_257065253.1	hypothetical protein	-
NQU59_RS05040 (NQU59_05040)	-	1058190..1058564 (-)	375	WP_257065254.1	hypothetical protein	-
NQU59_RS05045 (NQU59_05045)	-	1058565..1058975 (-)	411	WP_306436586.1	glycosyltransferase	-
NQU59_RS05050 (NQU59_05050)	-	1059229..1059705 (-)	477	WP_257065255.1	glycosyltransferase	-

Sequence

Protein

Download Length: 139 a.a. Molecular weight: 14336.29 Da Isoelectric Point: 9.0526

                                >NTDB_id=714396 NQU59_RS04985 WP_373463000.1 1049724..1050143(+) (pilA) [Acinetobacter colistiniresistens strain C-214]

MQKGFTLIELMIVVAIIGILAAIAIPAYQNYTKRSHVSEGLSLAAGAKAAVTEFYSSNGHWPTANASAGLATDSSITGNA

VKKVTVSGSAITVTYNDKVTDNGTIKLQGANTAGGVTWICTATGTNQPVDSKFLPSNCR

Nucleotide

Download Length: 420 bp

                                >NTDB_id=714396 NQU59_RS04985 WP_373463000.1 1049724..1050143(+) (pilA) [Acinetobacter colistiniresistens strain C-214]

ATGCAAAAGGGTTTTACTCTTATCGAATTGATGATCGTTGTCGCGATCATTGGTATCTTGGCTGCAATTGCGATTCCTGC

GTATCAAAACTATACGAAGCGTTCACACGTTTCTGAAGGTTTGAGTTTGGCTGCGGGTGCAAAAGCTGCGGTAACTGAGT

TCTACTCTTCTAATGGCCACTGGCCTACAGCGAACGCTTCTGCTGGTTTAGCAACAGATTCAAGTATTACGGGTAATGCT

GTTAAAAAAGTTACGGTCTCTGGTAGTGCAATTACTGTTACATATAATGACAAAGTTACTGATAATGGAACAATTAAATT

GCAAGGTGCTAATACTGCTGGTGGTGTAACCTGGATATGTACAGCAACTGGTACAAATCAACCTGTTGATAGCAAATTCT

TACCATCTAACTGTCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(34-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-24)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Ralstonia pseudosolanacearum GMI1000	44.643	100	0.54
pilE	Neisseria gonorrhoeae strain FA1090	46.795	100	0.525
pilE	Neisseria gonorrhoeae MS11	44.872	100	0.504
pilA2	Legionella pneumophila str. Paris	48.921	100	0.489
pilA2	Legionella pneumophila strain ERS1305867	47.101	99.281	0.468
comP	Acinetobacter baylyi ADP1	42.568	100	0.453
pilA	Haemophilus influenzae 86-028NP	45.652	99.281	0.453
pilA	Pseudomonas aeruginosa PAK	39.735	100	0.432
pilA/pilAII	Pseudomonas stutzeri DSM 10701	42.553	100	0.432
pilA	Haemophilus influenzae Rd KW20	43.478	99.281	0.432
pilA/pilA1	Eikenella corrodens VA1	36.076	100	0.41
pilA	Acinetobacter baumannii strain A118	39.161	100	0.403
pilA	Vibrio parahaemolyticus RIMD 2210633	40.741	97.122	0.396
pilA	Vibrio cholerae C6706	37.671	100	0.396
pilA	Vibrio cholerae strain A1552	37.671	100	0.396
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.671	100	0.396
pilA	Glaesserella parasuis strain SC1401	35.664	100	0.367

Detailed information NTG0714396