Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NP165_RS11460 Genome accession   NZ_CP102096
Coordinates   2444078..2445118 (-) Length   346 a.a.
NCBI ID   WP_257084086.1    Uniprot ID   -
Organism   Vibrio japonicus strain JCM 31412     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2439078..2450118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP165_RS11430 (NP165_11430) - 2439303..2440004 (+) 702 WP_257084080.1 endonuclease -
  NP165_RS11435 (NP165_11435) rsmE 2440109..2440840 (+) 732 WP_257084081.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NP165_RS11440 (NP165_11440) gshB 2440854..2441804 (+) 951 WP_257084082.1 glutathione synthase -
  NP165_RS11445 (NP165_11445) - 2441862..2442425 (+) 564 WP_257084083.1 YqgE/AlgH family protein -
  NP165_RS11450 (NP165_11450) ruvX 2442449..2442871 (+) 423 WP_257084084.1 Holliday junction resolvase RuvX -
  NP165_RS11455 (NP165_11455) pilU 2442960..2444066 (-) 1107 WP_257084085.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NP165_RS11460 (NP165_11460) pilT 2444078..2445118 (-) 1041 WP_257084086.1 type IV pilus twitching motility protein PilT Machinery gene
  NP165_RS11465 (NP165_11465) - 2445144..2445860 (+) 717 WP_257084087.1 YggS family pyridoxal phosphate-dependent enzyme -
  NP165_RS11470 (NP165_11470) proC 2445887..2446705 (+) 819 WP_257084088.1 pyrroline-5-carboxylate reductase -
  NP165_RS11475 (NP165_11475) - 2446742..2447299 (+) 558 WP_257084089.1 YggT family protein -
  NP165_RS11480 (NP165_11480) yggU 2447299..2447589 (+) 291 WP_257084090.1 DUF167 family protein YggU -
  NP165_RS11485 (NP165_11485) - 2447655..2448086 (+) 432 WP_257084091.1 DUF4426 domain-containing protein -
  NP165_RS11490 (NP165_11490) - 2448124..2448726 (+) 603 WP_257084092.1 XTP/dITP diphosphatase -
  NP165_RS11495 (NP165_11495) hemW 2448726..2449904 (+) 1179 WP_257084093.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38235.58 Da        Isoelectric Point: 5.9296

>NTDB_id=714363 NP165_RS11460 WP_257084086.1 2444078..2445118(-) (pilT) [Vibrio japonicus strain JCM 31412]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGDVRKLGVSAFSHSDVHRLVFEIMNDAQRSEFEERLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPQDIPTLEQIDAPEIFEKIANMEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKEMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAYGMQTMEQNG
AQLIAKGLVEPDEVAKKIGSESNSLF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=714363 NP165_RS11460 WP_257084086.1 2444078..2445118(-) (pilT) [Vibrio japonicus strain JCM 31412]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCGGGTGTTTCCCCAAT
GGTACGTATAGATGGAGATGTAAGGAAGCTTGGCGTATCAGCTTTTAGCCATTCTGATGTACATCGTTTGGTTTTTGAGA
TCATGAACGATGCACAGAGAAGTGAGTTTGAAGAGCGTTTAGAGGTCGATTTCTCTTTTGAGTTGCCAAACGTCGGGCGC
TTTCGTGTCAACGCGTTTAATCAAGCACGGGGCTGTGCAGCGGTATTTCGAACCATTCCTCAAGATATTCCAACACTAGA
ACAAATAGACGCACCAGAGATCTTCGAAAAGATCGCGAATATGGAAAAAGGCTTAGTGTTAGTCACAGGGCCGACGGGTT
CTGGTAAATCGACGACATTGGCGGCGATGGTCGATTATGTTAATCGCAATCATAACAAGCACATTCTAACTATCGAAGAT
CCGATCGAGTTTGTTCATACCAATAACAAATGTTTGATCAACCAGCGAGAAGTGCACCGTGATACGCACAGTTTTAACAA
TGCGTTGCGCAGCGCTTTACGTGAGGATCCGGATGTTATCCTCGTCGGTGAGCTTCGAGATCAAGAAACGATCAGTCTTG
CTTTAACAGCAGCGGAAACAGGCCACTTAGTGTTTGGTACGCTTCACACCAGCAGCGCAGCCAAAACGGTCGACCGTATT
ATTGATGTGTTTCCTGGCAGTGATAAAGAGATGGTACGCTCTATGTTGTCTGAATCACTGCGAGCGGTGATCGCGCAGAA
ACTGCTTAAGCGCATTGGTGGAGGTCGAACAGCTTGCCATGAAATCATGATGGCGACACCAGCGATTCGTAACCTAATCC
GTGAAGATAAAGTGGCTCAAATGTACTCGATCATTCAAACGGGGGCTGCGTATGGAATGCAAACCATGGAGCAGAATGGG
GCACAGTTGATTGCTAAAGGGTTAGTGGAGCCTGATGAGGTGGCAAAGAAAATCGGCTCCGAATCGAATAGTTTATTCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

89.645

97.688

0.876
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.645

97.688

0.876
  pilT Acinetobacter baumannii D1279779

70.091

95.665

0.671
  pilT Acinetobacter baumannii strain A118

70.091

95.665

0.671
  pilT Pseudomonas stutzeri DSM 10701

67.638

99.133

0.671
  pilT Acinetobacter nosocomialis M2

69.789

95.665

0.668
  pilT Acinetobacter baylyi ADP1

69.605

95.087

0.662
  pilT Pseudomonas aeruginosa PAK

67.359

97.399

0.656
  pilT Neisseria meningitidis 8013

64.451

100

0.645
  pilT Legionella pneumophila strain Lp02

67.477

95.087

0.642
  pilT Legionella pneumophila strain ERS1305867

67.477

95.087

0.642
  pilT Neisseria gonorrhoeae MS11

63.873

100

0.639
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.198

96.532

0.494
  pilU Vibrio cholerae strain A1552

42.462

93.931

0.399
  pilU Pseudomonas stutzeri DSM 10701

41.159

94.798

0.39
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364