Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NP165_RS11455 Genome accession   NZ_CP102096
Coordinates   2442960..2444066 (-) Length   368 a.a.
NCBI ID   WP_257084085.1    Uniprot ID   -
Organism   Vibrio japonicus strain JCM 31412     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2437960..2449066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP165_RS11425 (NP165_11425) - 2438727..2439224 (+) 498 WP_257084079.1 SprT family zinc-dependent metalloprotease -
  NP165_RS11430 (NP165_11430) - 2439303..2440004 (+) 702 WP_257084080.1 endonuclease -
  NP165_RS11435 (NP165_11435) rsmE 2440109..2440840 (+) 732 WP_257084081.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NP165_RS11440 (NP165_11440) gshB 2440854..2441804 (+) 951 WP_257084082.1 glutathione synthase -
  NP165_RS11445 (NP165_11445) - 2441862..2442425 (+) 564 WP_257084083.1 YqgE/AlgH family protein -
  NP165_RS11450 (NP165_11450) ruvX 2442449..2442871 (+) 423 WP_257084084.1 Holliday junction resolvase RuvX -
  NP165_RS11455 (NP165_11455) pilU 2442960..2444066 (-) 1107 WP_257084085.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NP165_RS11460 (NP165_11460) pilT 2444078..2445118 (-) 1041 WP_257084086.1 type IV pilus twitching motility protein PilT Machinery gene
  NP165_RS11465 (NP165_11465) - 2445144..2445860 (+) 717 WP_257084087.1 YggS family pyridoxal phosphate-dependent enzyme -
  NP165_RS11470 (NP165_11470) proC 2445887..2446705 (+) 819 WP_257084088.1 pyrroline-5-carboxylate reductase -
  NP165_RS11475 (NP165_11475) - 2446742..2447299 (+) 558 WP_257084089.1 YggT family protein -
  NP165_RS11480 (NP165_11480) yggU 2447299..2447589 (+) 291 WP_257084090.1 DUF167 family protein YggU -
  NP165_RS11485 (NP165_11485) - 2447655..2448086 (+) 432 WP_257084091.1 DUF4426 domain-containing protein -
  NP165_RS11490 (NP165_11490) - 2448124..2448726 (+) 603 WP_257084092.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41226.13 Da        Isoelectric Point: 6.8896

>NTDB_id=714362 NP165_RS11455 WP_257084085.1 2442960..2444066(-) (pilU) [Vibrio japonicus strain JCM 31412]
MDINIFLQGMNSQKASDLYITVGAPVLYRVDGELRAHGEKLTQSDVDNLLSSVLEEGRHKDFLQSKEANFAIVRDFGRYR
ISAFYQRELPGAVIRRIETTIPTFEDLRLPDVLQNLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNANRAGHILTIEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKAQFLFDLSMNLRGVVGQQLIRDKNGRGRHGVFEILLNSPRVSDLIRRGDLHELKSTMAKSKEFGMQTFDQSLY
QLVVDGKINEEDALHSADSANDLRIMLKTKRGETLAGGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=714362 NP165_RS11455 WP_257084085.1 2442960..2444066(-) (pilU) [Vibrio japonicus strain JCM 31412]
ATGGATATTAATATTTTCCTTCAGGGAATGAACAGTCAGAAGGCTTCGGATCTGTATATTACCGTTGGTGCGCCTGTGCT
TTATCGAGTGGATGGTGAGTTACGTGCCCATGGTGAAAAGCTAACACAGTCGGATGTAGACAATCTCCTATCATCGGTGC
TTGAAGAGGGGCGTCATAAAGATTTTCTACAAAGCAAAGAGGCGAATTTTGCAATCGTACGAGATTTTGGGCGCTACCGT
ATTTCTGCTTTTTACCAGCGCGAGTTGCCGGGCGCGGTCATTCGCCGTATTGAGACAACCATTCCAACGTTTGAAGATCT
TAGGCTCCCTGACGTTTTACAAAACCTTTCCATCGCGAAACGCGGTTTGGTGTTGGTGGTAGGCGCGACGGGGTCAGGGA
AATCGACAACGATGGCGGCGATGACAGGTTATCGGAATGCGAATAGGGCAGGTCATATTTTGACGATTGAAGATCCGATA
GAGTTCGTACATGAGCATAAGCGTTGTATCGTGACACAGCGAGAAGTCGGTTTAGATACGGAAAGCTATGAAGTCGCTTT
GAAGAACTCCCTGCGCCAAGCGCCCGATATGATTCTGATCGGTGAAATTCGTACTCGGGAAACCATGGAATATGCGATGA
CATTTGCTGAAACTGGGCATTTGTGCATGGCAACGCTCCATGCGAACAACGCTAACCAAGCGCTTGAGCGTATTCTGCAT
CTTGTCCCAAAAGAGCAGAAAGCCCAGTTTTTGTTTGATCTTTCGATGAACTTACGTGGCGTCGTTGGTCAACAGCTGAT
CCGAGATAAGAATGGGCGAGGTCGTCATGGTGTATTCGAGATCCTACTCAACAGCCCACGAGTATCGGATTTGATCCGTC
GTGGCGATCTGCATGAATTGAAATCCACGATGGCGAAGTCGAAAGAGTTTGGCATGCAGACTTTCGATCAATCTTTATAT
CAGCTGGTGGTTGATGGGAAGATCAATGAGGAGGATGCACTCCATAGCGCGGATTCTGCCAATGATCTTCGCATTATGCT
TAAGACTAAGAGAGGAGAAACTCTTGCTGGTGGCTCTTTGGCAAATGTGAAGATTGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

80.163

100

0.802
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

51.567

95.38

0.492
  pilT Pseudomonas aeruginosa PAK

42.478

92.12

0.391
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.519

88.043

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Neisseria meningitidis 8013

39.13

93.75

0.367
  pilT Neisseria gonorrhoeae MS11

39.13

93.75

0.367