Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NOX82_RS02450 Genome accession   NZ_CP101752
Coordinates   546576..547610 (+) Length   344 a.a.
NCBI ID   WP_009615237.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain C12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541576..552610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOX82_RS02425 (NOX82_02425) - 541859..543826 (-) 1968 WP_256513972.1 dynamin-like GTPase family protein -
  NOX82_RS02430 (NOX82_02430) - 543938..544234 (-) 297 WP_009615233.1 DUF167 domain-containing protein -
  NOX82_RS02435 (NOX82_02435) - 544374..544967 (-) 594 WP_009615234.1 YggT family protein -
  NOX82_RS02440 (NOX82_02440) proC 544978..545796 (-) 819 WP_256513974.1 pyrroline-5-carboxylate reductase -
  NOX82_RS02445 (NOX82_02445) - 545813..546502 (-) 690 WP_256513975.1 YggS family pyridoxal phosphate-dependent enzyme -
  NOX82_RS02450 (NOX82_02450) pilT 546576..547610 (+) 1035 WP_009615237.1 type IV pilus twitching motility protein PilT Machinery gene
  NOX82_RS02455 (NOX82_02455) pilU 547715..548860 (+) 1146 WP_074977840.1 type IV pilus ATPase PilU Machinery gene
  NOX82_RS02460 (NOX82_02460) - 549631..550035 (+) 405 WP_074986418.1 TM2 domain-containing protein -
  NOX82_RS02465 (NOX82_02465) - 550179..551552 (+) 1374 WP_256513976.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37852.73 Da        Isoelectric Point: 6.7752

>NTDB_id=711960 NOX82_RS02450 WP_009615237.1 546576..547610(+) (pilT) [Pseudomonas citronellolis strain C12]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KGLVAKGLVTRDAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=711960 NOX82_RS02450 WP_009615237.1 546576..547610(+) (pilT) [Pseudomonas citronellolis strain C12]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAAGGCGCTTCGGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTGGAAACCGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
GGATCTGGGCATGGGCGAAGTGTTCAAGCGGGTTTCCGACGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCTCGCTTGCGGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGGGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAGAAGGCCATGGTCCGTTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGGTGACCCGCGACGCCGCCAAGGAAAAGGCGAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.93

100

0.959
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395