Detailed information NTG0711961

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NOX82_RS02455
• Genome accession: NZ_CP101752
• Coordinates: 547715..548860 (+)
• Length: 381 a.a.
• NCBI ID: WP_074977840.1
• Uniprot ID: -
• Organism: Pseudomonas citronellolis strain C12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 542715..553860

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NOX82_RS02430 (NOX82_02430)	-	543938..544234 (-)	297	WP_009615233.1	DUF167 domain-containing protein	-
NOX82_RS02435 (NOX82_02435)	-	544374..544967 (-)	594	WP_009615234.1	YggT family protein	-
NOX82_RS02440 (NOX82_02440)	proC	544978..545796 (-)	819	WP_256513974.1	pyrroline-5-carboxylate reductase	-
NOX82_RS02445 (NOX82_02445)	-	545813..546502 (-)	690	WP_256513975.1	YggS family pyridoxal phosphate-dependent enzyme	-
NOX82_RS02450 (NOX82_02450)	pilT	546576..547610 (+)	1035	WP_009615237.1	type IV pilus twitching motility protein PilT	Machinery gene
NOX82_RS02455 (NOX82_02455)	pilU	547715..548860 (+)	1146	WP_074977840.1	type IV pilus ATPase PilU	Machinery gene
NOX82_RS02460 (NOX82_02460)	-	549631..550035 (+)	405	WP_074986418.1	TM2 domain-containing protein	-
NOX82_RS02465 (NOX82_02465)	-	550179..551552 (+)	1374	WP_256513976.1	pyridoxal-phosphate dependent enzyme	-
NOX82_RS02470 (NOX82_02470)	-	551549..552721 (+)	1173	WP_256513978.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42671.03 Da
• Isoelectric Point: 6.7791

>NTDB_id=711961 NOX82_RS02455 WP_074977840.1 547715..548860(+) (pilU) [Pseudomonas citronellolis strain C12]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKVLPVTKTPLSPEQTRETVLAVMNEQQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMEHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHQQIWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSSMTQGLTLELSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=711961 NOX82_RS02455 WP_074977840.1 547715..548860(+) (pilU) [Pseudomonas citronellolis strain C12]
ATGGAATTCGAGAAGCTTCTGCGGCTGATGGTGGAGAAGGGCGCCTCGGACCTCTTCGTCACCGCCGGCGTGCCCCCGTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCCCGCTGTCGCCCGAGCAGACCCGCGAGACGGTGCTGGCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGCACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGGCGC
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCATCGA
GGACCTCAAGCTGCCGGAGATCCTCAAGAAGCTGGCCATGACCAAGCGCGGGCTGGTGATCTTCGTCGGTGCCACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACACCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGAGCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACCAGCAGATCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGTTGAATACCCCGCTGGCCGCTGACCTGA
TCCGCAAGGGTGAGGTGCATGAGCTGAAGCCGTTGATGAAGCGTTCCACCGAGCAGGGTATGCAGACCTTCGATCAGGCG
CTGTATCAGCTCTATTCCCAGGGGGAGATCACCTATGAGGATGCGCTGGCCTATGCTGATTCGGCCAACGATCTGCGGTT
GATGATCAAGCTTGGGTCGGAGACCGATGCGGATCATCTGTCGAGCATGACTCAGGGGCTGACCCTGGAGCTGAGTGACG
ACGATCCGAACCGTCGTTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.927	100	0.879
pilU	Acinetobacter baylyi ADP1	63.032	98.688	0.622
pilU	Vibrio cholerae strain A1552	60.571	91.864	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386