Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NPN27_RS20710 Genome accession   NZ_CP101739
Coordinates   4408481..4409515 (-) Length   344 a.a.
NCBI ID   WP_011914970.1    Uniprot ID   A0A0H3YZL0
Organism   Stutzerimonas stutzeri strain J15     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4403481..4414515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPN27_RS20690 (NPN27_20690) - 4404701..4405096 (-) 396 WP_011914966.1 NINE protein -
  NPN27_RS20695 (NPN27_20695) - 4405232..4406446 (+) 1215 WP_049340316.1 putative DNA modification/repair radical SAM protein -
  NPN27_RS20700 (NPN27_20700) - 4406446..4407279 (+) 834 WP_019405533.1 TIGR03915 family putative DNA repair protein -
  NPN27_RS20705 (NPN27_20705) pilU 4407276..4408421 (-) 1146 WP_019405534.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NPN27_RS20710 (NPN27_20710) pilT 4408481..4409515 (-) 1035 WP_011914970.1 type IV pilus twitching motility protein PilT Machinery gene
  NPN27_RS20715 (NPN27_20715) - 4409582..4410280 (+) 699 WP_256518142.1 YggS family pyridoxal phosphate-dependent enzyme -
  NPN27_RS20720 (NPN27_20720) proC 4410291..4411115 (+) 825 WP_256518143.1 pyrroline-5-carboxylate reductase -
  NPN27_RS20725 (NPN27_20725) - 4411125..4411715 (+) 591 WP_014598006.1 YggT family protein -
  NPN27_RS20730 (NPN27_20730) - 4411985..4413952 (+) 1968 WP_125842299.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38073.88 Da        Isoelectric Point: 6.8359

>NTDB_id=711887 NPN27_RS20710 WP_011914970.1 4408481..4409515(-) (pilT) [Stutzerimonas stutzeri strain J15]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLNMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLLAKGLISRDSAREKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=711887 NPN27_RS20710 WP_011914970.1 4408481..4409515(-) (pilT) [Stutzerimonas stutzeri strain J15]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGTGACGTGCGGCGCATCAATCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAAACCGACTTCTCGTTCGAGGTGCCGGGTGTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
GGACCTCAACATGGGTGAGGTCTTCCGCAAGATCACCGACGTGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGCT
CGGGTAAGTCGACCACCCTGGCGGCGATGCTCGATTACCTGAACAACACCAAATACCACCATATCCTTACCATCGAAGAC
CCCATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCGCTGCGCTCGGCCTTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTCGTGTTCGGCACGCTGCACACCACCTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAAAAATCCATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGTGGCGGCCGCGTCGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGTTCGCTTGGCATGCAGACCCTGGACATGTGCCTC
AAGGGGCTGCTGGCCAAGGGCCTGATCAGCCGCGACAGCGCGCGGGAAAAGGCCAAGCAGCCCGAGAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3YZL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

97.965

100

0.98
  pilT Pseudomonas aeruginosa PAK

95.058

100

0.951
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter nosocomialis M2

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

83.721

100

0.837
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392