Detailed information NTG0711886

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NPN27_RS20705
• Genome accession: NZ_CP101739
• Coordinates: 4407276..4408421 (-)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain J15
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4402276..4413421

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NPN27_RS20680 (NPN27_20680)	-	4402353..4403369 (+)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-
NPN27_RS20685 (NPN27_20685)	-	4403369..4404643 (+)	1275	WP_256518141.1	dihydroorotase	-
NPN27_RS20690 (NPN27_20690)	-	4404701..4405096 (-)	396	WP_011914966.1	NINE protein	-
NPN27_RS20695 (NPN27_20695)	-	4405232..4406446 (+)	1215	WP_049340316.1	putative DNA modification/repair radical SAM protein	-
NPN27_RS20700 (NPN27_20700)	-	4406446..4407279 (+)	834	WP_019405533.1	TIGR03915 family putative DNA repair protein	-
NPN27_RS20705 (NPN27_20705)	pilU	4407276..4408421 (-)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NPN27_RS20710 (NPN27_20710)	pilT	4408481..4409515 (-)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
NPN27_RS20715 (NPN27_20715)	-	4409582..4410280 (+)	699	WP_256518142.1	YggS family pyridoxal phosphate-dependent enzyme	-
NPN27_RS20720 (NPN27_20720)	proC	4410291..4411115 (+)	825	WP_256518143.1	pyrroline-5-carboxylate reductase	-
NPN27_RS20725 (NPN27_20725)	-	4411125..4411715 (+)	591	WP_014598006.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=711886 NPN27_RS20705 WP_019405534.1 4407276..4408421(-) (pilU) [Stutzerimonas stutzeri strain J15]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=711886 NPN27_RS20705 WP_019405534.1 4407276..4408421(-) (pilU) [Stutzerimonas stutzeri strain J15]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACGTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTCATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391