Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFC81_RS00365 Genome accession   NZ_CP101717
Coordinates   80563..81597 (+) Length   344 a.a.
NCBI ID   WP_304995548.1    Uniprot ID   A0AB38YG37
Organism   Salinispirillum sp. LH 10-3-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 75563..86597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFC81_RS00335 (NFC81_00335) - 75983..76450 (-) 468 WP_304995542.1 DUF4426 domain-containing protein -
  NFC81_RS00340 (NFC81_00340) metW 76447..77043 (-) 597 WP_304995543.1 methionine biosynthesis protein MetW -
  NFC81_RS00345 (NFC81_00345) - 77033..78148 (-) 1116 WP_370529917.1 homoserine O-acetyltransferase -
  NFC81_RS00350 (NFC81_00350) - 78298..78885 (-) 588 WP_304995545.1 YggT family protein -
  NFC81_RS00355 (NFC81_00355) proC 78895..79731 (-) 837 WP_304995546.1 pyrroline-5-carboxylate reductase -
  NFC81_RS00360 (NFC81_00360) - 79728..80471 (-) 744 WP_304995547.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFC81_RS00365 (NFC81_00365) pilT 80563..81597 (+) 1035 WP_304995548.1 type IV pilus twitching motility protein PilT Machinery gene
  NFC81_RS00370 (NFC81_00370) pilU 81610..82737 (+) 1128 WP_304995549.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NFC81_RS00375 (NFC81_00375) - 82814..83206 (+) 393 WP_304995550.1 hypothetical protein -
  NFC81_RS00380 (NFC81_00380) trmB 83260..83955 (-) 696 WP_304995551.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  NFC81_RS00385 (NFC81_00385) - 83945..84310 (-) 366 WP_304995552.1 DUF423 domain-containing protein -
  NFC81_RS00390 (NFC81_00390) rpoH 84400..85266 (-) 867 WP_304995553.1 RNA polymerase sigma factor RpoH -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38127.62 Da        Isoelectric Point: 6.6423

>NTDB_id=711699 NFC81_RS00365 WP_304995548.1 80563..81597(+) (pilT) [Salinispirillum sp. LH 10-3-1]
MDITELLAFSAKQNASDLHLSANLPPMIRVDGDVRRINLPAMPHKEVHGLIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGEVFKDIAAAPRGLVLVTGPTGSGKSTTLAAMINHINEHRYEHVLTVED
PIEFVHESKRCLVNQREVGRDTLGFNEALRSALREDPDIILVGEMRDIETIRLALTAAETGHVVFGTLHTTSAAKTIDRI
IDVFPAEEKSMVRSMLSESLQAVISQTLLKRKGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGASQGMITMDQSL
QNLVQKGIITSEEARHKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=711699 NFC81_RS00365 WP_304995548.1 80563..81597(+) (pilT) [Salinispirillum sp. LH 10-3-1]
ATGGATATTACAGAACTCTTAGCGTTCAGCGCCAAACAGAACGCATCTGACTTGCATTTGTCCGCCAATTTGCCACCCAT
GATTCGTGTCGATGGCGACGTTCGCCGCATTAATTTGCCCGCCATGCCGCACAAAGAAGTGCATGGTTTGATCTATGACA
TCATGAACGACAAGCAGCGCAAAGATTACGAGGAGTTTCTGGAGACCGACTTCTCCTTTGAGGTACCCGGTGTGGCGCGT
TTTCGTGTTAACGCGTTCAATCACAACCGTGGCGCTGGCGCCGTATTCCGTACCATCCCCTCCAAGGTATTGACCATGGA
AGATTTGGGCATGGGCGAGGTCTTCAAGGACATCGCCGCTGCTCCGCGTGGGCTTGTCTTGGTAACGGGCCCAACTGGCT
CTGGTAAGTCAACCACCTTGGCGGCGATGATCAACCACATTAACGAACATCGCTATGAGCACGTGTTGACGGTGGAAGAC
CCCATCGAGTTCGTACACGAAAGCAAGCGCTGCCTAGTGAACCAGCGCGAAGTGGGGCGCGATACGTTGGGCTTCAACGA
AGCTCTGCGATCGGCGCTGCGGGAAGACCCGGACATCATTCTGGTGGGTGAGATGCGAGACATTGAAACCATCCGTCTGG
CGTTGACGGCGGCAGAAACCGGCCACGTGGTATTCGGCACGCTGCACACCACCTCAGCGGCCAAGACCATCGACCGTATC
ATCGACGTTTTTCCCGCCGAGGAAAAATCCATGGTTCGCTCAATGCTGTCAGAATCGTTACAAGCCGTCATATCGCAAAC
TCTGCTTAAACGTAAAGGCGGCGGGCGTGTGGCAGCCCACGAGATCATGCGCGGCACCTCGGCGATTCGAAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACAGCGCGATCCAAACCGGCGCGTCGCAAGGCATGATTACCATGGATCAGTCGTTA
CAAAATCTGGTTCAAAAGGGCATCATTACGTCAGAAGAAGCCCGTCATAAAGCCAAGTCACCGGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.593

100

0.846
  pilT Pseudomonas aeruginosa PAK

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

82.267

100

0.823
  pilT Acinetobacter baumannii D1279779

82.267

100

0.823
  pilT Acinetobacter baumannii strain A118

82.267

100

0.823
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.401

100

0.41
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387