Detailed information NTG0711571

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: POF58_RS04280
• Genome accession: NZ_CP116711
• Coordinates: 782869..783522 (+)
• Length: 217 a.a.
• NCBI ID: WP_000461742.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 777869..788522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF58_RS04255 (POF58_04255)	-	778036..778866 (+)	831	WP_000735512.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
POF58_RS04260 (POF58_04260)	-	779101..780687 (+)	1587	WP_000673082.1	DEAD/DEAH box helicase	-
POF58_RS04265 (POF58_04265)	-	780872..781138 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
POF58_RS04270 (POF58_04270)	-	781131..781895 (-)	765	WP_000611114.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
POF58_RS04275 (POF58_04275)	-	782030..782770 (+)	741	WP_000500220.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
POF58_RS04280 (POF58_04280)	comEA/celA/cilE	782869..783522 (+)	654	WP_000461742.1	helix-hairpin-helix domain-containing protein	Machinery gene
POF58_RS04285 (POF58_04285)	comEC/celB	783506..785743 (+)	2238	WP_000939909.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
POF58_RS04290 (POF58_04290)	-	785869..786678 (+)	810	WP_000153219.1	Cof-type HAD-IIB family hydrolase	-
POF58_RS04295 (POF58_04295)	-	786689..787633 (+)	945	WP_000200830.1	LacI family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 217 a.a.
• Molecular weight: 23728.08 Da
• Isoelectric Point: 9.2366

>NTDB_id=711571 POF58_RS04280 WP_000461742.1 782869..783522(+) (comEA/celA/cilE) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLTNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide

• Length: 654 bp

>NTDB_id=711571 POF58_RS04280 WP_000461742.1 782869..783522(+) (comEA/celA/cilE) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTATTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACGATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAACCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCCACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGGGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACAATTGACTAA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (153-215)

• SLBB domain (SLBB) (75-128)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	45.045	100	0.461
comEA/celA/cilE	Streptococcus pneumoniae Rx1	44.144	100	0.452
comEA/celA/cilE	Streptococcus pneumoniae D39	44.144	100	0.452
comEA/celA/cilE	Streptococcus pneumoniae R6	44.144	100	0.452
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.694	100	0.447
comEA/celA/cilE	Streptococcus mitis SK321	43.636	100	0.442
comEA	Lactococcus lactis subsp. cremoris KW2	39.462	100	0.406
comEA	Streptococcus thermophilus LMD-9	55.195	70.968	0.392
comEA	Latilactobacillus sakei subsp. sakei 23K	33.054	100	0.364