Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NM620_RS02240 Genome accession   NZ_CP101694
Coordinates   443719..444756 (+) Length   345 a.a.
NCBI ID   WP_256383624.1    Uniprot ID   -
Organism   Photobacterium toruni strain WD2103     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438719..449756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM620_RS02205 - 438729..439127 (+) 399 WP_080174536.1 DUF2884 family protein -
  NM620_RS02210 hemW 439246..440412 (-) 1167 WP_256383622.1 radical SAM family heme chaperone HemW -
  NM620_RS02215 - 440416..441006 (-) 591 WP_256383623.1 XTP/dITP diphosphatase -
  NM620_RS02220 yggU 441206..441502 (-) 297 WP_045037795.1 DUF167 family protein YggU -
  NM620_RS02225 - 441499..442056 (-) 558 WP_045037796.1 YggT family protein -
  NM620_RS02230 proC 442066..442884 (-) 819 WP_080174539.1 pyrroline-5-carboxylate reductase -
  NM620_RS02235 - 442983..443693 (-) 711 WP_080174540.1 YggS family pyridoxal phosphate-dependent enzyme -
  NM620_RS02240 pilT 443719..444756 (+) 1038 WP_256383624.1 type IV pilus twitching motility protein PilT Machinery gene
  NM620_RS02245 pilU 444773..445879 (+) 1107 WP_080174542.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NM620_RS02250 ruvX 446236..446667 (-) 432 WP_256383625.1 Holliday junction resolvase RuvX -
  NM620_RS02255 - 446664..447227 (-) 564 WP_080174544.1 YqgE/AlgH family protein -
  NM620_RS02260 gshB 447348..448298 (-) 951 WP_256383626.1 glutathione synthase -
  NM620_RS02265 rsmE 448316..449047 (-) 732 WP_080174546.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38125.61 Da        Isoelectric Point: 6.1261

>NTDB_id=711571 NM620_RS02240 WP_256383624.1 443719..444756(+) (pilT) [Photobacterium toruni strain WD2103]
MDITELLDFSVKHNASDLHLSAGVPPIIRVDGDVRKLSLPALTHTEVHQLIFDIMNDAQRREYEEKLEVDFSFELPDVGR
FRVNAFHQSRGASAVLRTIPMNIPTLASLNVPEVFYDIAQLNRGLVLVTGATGSGKSTTIAALVDYINSNYQRHILTIED
PIEFVHTSKSCLINQREVHRDTLSFQNALRSSLREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGNDKSMVRSMLSESLRAVVAQNLLKCTSGGRVACHEVMMATPAIRNLIREDKIAQMYSMIQTGSSLGMQTMEQSV
KMLVAQGMVAAEEGRRILDSNQRGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=711571 NM620_RS02240 WP_256383624.1 443719..444756(+) (pilT) [Photobacterium toruni strain WD2103]
ATGGATATTACCGAATTACTCGATTTTAGTGTAAAGCATAATGCATCAGATCTGCACCTTTCTGCGGGTGTTCCACCAAT
AATAAGGGTGGATGGTGATGTGAGAAAGCTCAGTTTACCTGCTTTAACTCATACAGAAGTGCACCAATTGATCTTTGATA
TTATGAACGATGCACAGCGTCGAGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCCGACGTTGGTCGT
TTCAGGGTTAATGCTTTTCATCAATCTCGTGGTGCTTCTGCTGTACTACGAACTATCCCTATGAATATTCCTACGTTAGC
ATCACTGAATGTTCCTGAGGTTTTTTATGATATTGCGCAGCTTAACCGTGGGTTAGTACTGGTCACGGGCGCAACGGGCT
CAGGTAAATCAACCACCATTGCCGCCTTAGTAGATTACATCAATAGCAACTATCAACGCCATATATTGACCATTGAAGAT
CCGATCGAATTTGTCCATACCAGTAAAAGTTGTCTGATCAATCAACGTGAAGTGCATCGCGATACCTTAAGTTTCCAAAA
TGCACTGCGATCATCATTGCGTGAAGATCCCGATGTGATTGTCGTGGGTGAGTTGCGTGATCAAGAAACCATTAGTTTGG
CATTAACGGCAGCTGAAACCGGTCACTTGGTATTAGGTACTTTGCATACCAGTTCAGCAGCTAAAACGGTTGACCGTATT
ATTGATGTTTTTCCTGGTAATGATAAATCGATGGTGCGCTCAATGTTGTCAGAATCATTGCGCGCCGTTGTGGCACAAAA
CTTATTGAAGTGTACCTCTGGTGGTCGTGTTGCGTGTCATGAAGTGATGATGGCAACGCCGGCTATTCGTAATTTAATCC
GTGAAGATAAAATTGCTCAGATGTATTCGATGATTCAAACCGGCTCATCACTTGGTATGCAGACCATGGAACAAAGTGTA
AAAATGCTGGTGGCACAAGGAATGGTAGCAGCCGAAGAAGGGCGTCGTATTCTTGATAGTAATCAACGTGGATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.652

100

0.757
  pilT Vibrio cholerae strain A1552

75.652

100

0.757
  pilT Acinetobacter baylyi ADP1

67.544

99.13

0.67
  pilT Acinetobacter baumannii strain A118

68.955

97.101

0.67
  pilT Acinetobacter baumannii D1279779

68.955

97.101

0.67
  pilT Pseudomonas stutzeri DSM 10701

68.657

97.101

0.667
  pilT Pseudomonas aeruginosa PAK

68.657

97.101

0.667
  pilT Acinetobacter nosocomialis M2

68.657

97.101

0.667
  pilT Legionella pneumophila strain Lp02

68.485

95.652

0.655
  pilT Legionella pneumophila strain ERS1305867

68.485

95.652

0.655
  pilT Neisseria meningitidis 8013

64.095

97.681

0.626
  pilT Neisseria gonorrhoeae MS11

63.798

97.681

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.837

99.71

0.487
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.642

98.841

0.412
  pilU Acinetobacter baylyi ADP1

39.752

93.333

0.371