Detailed information NTG0711572

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NM620_RS02245
• Genome accession: NZ_CP101694
• Coordinates: 444773..445879 (+)
• Length: 368 a.a.
• NCBI ID: WP_080174542.1
• Uniprot ID: -
• Organism: Photobacterium toruni strain WD2103
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 439773..450879

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NM620_RS02215	-	440416..441006 (-)	591	WP_256383623.1	XTP/dITP diphosphatase	-
NM620_RS02220	yggU	441206..441502 (-)	297	WP_045037795.1	DUF167 family protein YggU	-
NM620_RS02225	-	441499..442056 (-)	558	WP_045037796.1	YggT family protein	-
NM620_RS02230	proC	442066..442884 (-)	819	WP_080174539.1	pyrroline-5-carboxylate reductase	-
NM620_RS02235	-	442983..443693 (-)	711	WP_080174540.1	YggS family pyridoxal phosphate-dependent enzyme	-
NM620_RS02240	pilT	443719..444756 (+)	1038	WP_256383624.1	type IV pilus twitching motility protein PilT	Machinery gene
NM620_RS02245	pilU	444773..445879 (+)	1107	WP_080174542.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NM620_RS02250	ruvX	446236..446667 (-)	432	WP_256383625.1	Holliday junction resolvase RuvX	-
NM620_RS02255	-	446664..447227 (-)	564	WP_080174544.1	YqgE/AlgH family protein	-
NM620_RS02260	gshB	447348..448298 (-)	951	WP_256383626.1	glutathione synthase	-
NM620_RS02265	rsmE	448316..449047 (-)	732	WP_080174546.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
NM620_RS02270	-	449281..449991 (-)	711	WP_256383627.1	endonuclease	-
NM620_RS02275	-	450115..450606 (-)	492	WP_256383628.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 41276.25 Da
• Isoelectric Point: 5.5848

>NTDB_id=711572 NM620_RS02245 WP_080174542.1 444773..445879(+) (pilU) [Photobacterium toruni strain WD2103]
MTLDDILEQVVIKKASDLYITVDSACLLKIDGNLHDIGKVLTRDDVDSLFDQAMDANLRQEFISTKEANFALVRNEHRFR
VSAFWQRELPGMVIRRIESQIPQISDLQLPIEMERLALSKRGLVLIVGATGSGKSTSMAAMTGYRNQHRSGHILTVEDPI
EFVHKHNKCIVTQREVGLDTASYEVALKNSLRQAPDMILIGEIRTRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPEERRDQFLFDLSLNLKCILAQQLVADANGVGRHGVYELLLNTPRVADLIRRGDLYEIKDVMAKSTDAGMITFDQSLY
ALFSEGKITEQDALYHADSANDLRLMIKLGDHSIQPLSSLSGVTLENS

Nucleotide

• Length: 1107 bp

>NTDB_id=711572 NM620_RS02245 WP_080174542.1 444773..445879(+) (pilU) [Photobacterium toruni strain WD2103]
ATGACGTTAGATGACATTCTTGAACAGGTTGTTATTAAAAAAGCTTCTGATTTATATATTACGGTTGATTCAGCGTGTTT
ATTAAAAATTGACGGTAATCTGCATGATATAGGCAAGGTATTAACCCGTGATGATGTTGATAGTTTGTTTGATCAAGCGA
TGGATGCCAACCTCAGACAGGAGTTTATCAGTACCAAAGAAGCTAATTTTGCTTTAGTGCGTAATGAACATCGATTTCGT
GTCAGTGCTTTTTGGCAACGAGAATTACCCGGAATGGTGATCCGTCGTATTGAATCTCAAATTCCGCAAATAAGTGATTT
GCAGTTACCGATTGAGATGGAGCGATTAGCATTATCGAAACGGGGTTTGGTATTAATTGTCGGGGCGACAGGTTCTGGTA
AATCAACCTCAATGGCAGCCATGACGGGCTATCGTAATCAGCATCGAAGTGGGCATATTTTAACGGTTGAAGATCCAATT
GAATTTGTTCATAAACACAACAAATGCATTGTCACTCAGCGTGAAGTTGGGCTGGACACTGCTAGCTATGAAGTGGCACT
AAAAAATTCATTACGCCAAGCACCAGATATGATTTTGATTGGTGAAATTCGTACTCGTGAAACGATGGAATATGCGATGA
ATTTTGCTGAAACTGGCCATTTATGTATGGCAACGTTGCATGCCAATAATGCTAATCAGGCATTAGAGCGAATTTTACAT
TTAGTACCTGAAGAACGACGCGATCAATTTTTATTTGATTTATCTCTTAATCTTAAATGTATTTTGGCGCAGCAGTTAGT
TGCTGATGCTAATGGTGTTGGTCGCCATGGTGTGTATGAATTATTACTGAATACACCGCGAGTTGCCGATTTAATTCGAC
GGGGTGATTTGTATGAAATTAAAGATGTGATGGCGAAATCAACGGATGCAGGAATGATAACGTTTGACCAGTCATTGTAT
GCGTTATTTAGCGAAGGAAAAATAACTGAGCAAGATGCGCTGTACCATGCCGACTCCGCTAATGATTTACGGTTAATGAT
TAAATTGGGTGATCATAGTATTCAGCCGTTAAGTAGTTTATCGGGAGTAACGCTTGAAAACAGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (66-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	70.115	94.565	0.663
pilU	Pseudomonas stutzeri DSM 10701	57.979	100	0.592
pilU	Acinetobacter baylyi ADP1	54.42	98.37	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.653	91.576	0.372