NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	NMK59_RS22770	Genome accession	NZ_CP101302
Coordinates	4526845..4527825 (-)	Length	326 a.a.
NCBI ID	WP_001055631.1	Uniprot ID	A0A3E1VZY4
Organism	Escherichia coli strain 2000-3039
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4521845..4532825

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMK59_RS22740 (NMK59_22740)	yggI	4522495..4522992 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
NMK59_RS22745 (NMK59_22745)	endA	4523087..4523794 (+)	708	WP_000286500.1	deoxyribonuclease I	-
NMK59_RS22750 (NMK59_22750)	rsmE	4523874..4524605 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
NMK59_RS22755 (NMK59_22755)	gshB	4524618..4525568 (+)	951	WP_000593273.1	glutathione synthase	-
NMK59_RS22760 (NMK59_22760)	yqgE	4525677..4526240 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
NMK59_RS22765 (NMK59_22765)	ruvX	4526240..4526656 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
NMK59_RS22770 (NMK59_22770)	pilT	4526845..4527825 (-)	981	WP_001055631.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NMK59_RS22775 (NMK59_22775)	yggS	4527843..4528547 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
NMK59_RS22780 (NMK59_22780)	yggT	4528565..4529131 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
NMK59_RS22785 (NMK59_22785)	yggU	4529128..4529418 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
NMK59_RS22790 (NMK59_22790)	rdgB	4529426..4530019 (+)	594	WP_001174731.1	XTP/dITP diphosphatase	-
NMK59_RS22795 (NMK59_22795)	hemW	4530012..4531148 (+)	1137	WP_000239917.1	radical SAM family heme chaperone HemW	-
NMK59_RS22800 (NMK59_22800)	-	4531460..4532446 (+)	987	WP_000784013.1	TRAP transporter substrate-binding protein	-

Sequence

Protein

Download Length: 326 a.a. Molecular weight: 35946.11 Da Isoelectric Point: 5.7980

                                >NTDB_id=709568 NMK59_RS22770 WP_001055631.1 4526845..4527825(-) (pilT) [Escherichia coli strain 2000-3039]

MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG

SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE

YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS

FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR

VGEGRL

Nucleotide

Download Length: 981 bp

                                >NTDB_id=709568 NMK59_RS22770 WP_001055631.1 4526845..4527825(-) (pilT) [Escherichia coli strain 2000-3039]

ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG

ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG

ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC

AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG

CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT

CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA

TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG

GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG

CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA

TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT

GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA

AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG

GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A3E1VZY4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362

Detailed information NTG0709568