Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PIC88_RS19040 Genome accession   NZ_CP116188
Coordinates   3900626..3901606 (-) Length   326 a.a.
NCBI ID   WP_072023963.1    Uniprot ID   -
Organism   Escherichia coli strain DETEC-E223     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3895626..3906606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIC88_RS19010 (PIC88_19005) yggI 3896289..3896786 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  PIC88_RS19015 (PIC88_19010) endA 3896881..3897588 (+) 708 WP_000286500.1 deoxyribonuclease I -
  PIC88_RS19020 (PIC88_19015) rsmE 3897668..3898399 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PIC88_RS19025 (PIC88_19020) gshB 3898412..3899362 (+) 951 WP_000593273.1 glutathione synthase -
  PIC88_RS19030 (PIC88_19025) yqgE 3899471..3900034 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  PIC88_RS19035 (PIC88_19030) ruvX 3900034..3900450 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  PIC88_RS19040 (PIC88_19035) pilT 3900626..3901606 (-) 981 WP_072023963.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PIC88_RS19045 (PIC88_19040) yggS 3901624..3902328 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  PIC88_RS19050 (PIC88_19045) yggT 3902346..3902912 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  PIC88_RS19055 (PIC88_19050) yggU 3902909..3903199 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  PIC88_RS19060 (PIC88_19055) rdgB 3903207..3903800 (+) 594 WP_001174735.1 XTP/dITP diphosphatase -
  PIC88_RS19065 (PIC88_19060) hemW 3903793..3904929 (+) 1137 WP_000239916.1 radical SAM family heme chaperone HemW -
  PIC88_RS19070 (PIC88_19065) yggM 3905084..3906091 (-) 1008 WP_000745210.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35970.17 Da        Isoelectric Point: 5.7654

>NTDB_id=708924 PIC88_RS19040 WP_072023963.1 3900626..3901606(-) (pilT) [Escherichia coli strain DETEC-E223]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLTAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPVQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708924 PIC88_RS19040 WP_072023963.1 3900626..3901606(-) (pilT) [Escherichia coli strain DETEC-E223]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAGTTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGACGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGTGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCTGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.343

100

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362