Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NGC85_RS12365 Genome accession   NZ_CP101115
Coordinates   2635149..2636186 (+) Length   345 a.a.
NCBI ID   WP_254731571.1    Uniprot ID   -
Organism   Acinetobacter sp. Z1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2630149..2641186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGC85_RS12345 (NGC85_12345) - 2632326..2632613 (+) 288 WP_254805600.1 hypothetical protein -
  NGC85_RS12350 (NGC85_12350) - 2632685..2633359 (+) 675 WP_254805601.1 HAD family hydrolase -
  NGC85_RS12355 (NGC85_12355) - 2633413..2634149 (-) 737 Protein_2412 hypothetical protein -
  NGC85_RS12360 (NGC85_12360) - 2634336..2635022 (-) 687 WP_254731569.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGC85_RS12365 (NGC85_12365) pilT 2635149..2636186 (+) 1038 WP_254731571.1 type IV pilus twitching motility protein PilT Machinery gene
  NGC85_RS12370 (NGC85_12370) pilU 2636211..2637329 (+) 1119 WP_254731573.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGC85_RS12375 (NGC85_12375) fur 2637424..2637861 (-) 438 WP_005056466.1 ferric iron uptake transcriptional regulator -
  NGC85_RS12380 (NGC85_12380) - 2637972..2638370 (+) 399 WP_005056468.1 outer membrane protein assembly factor BamE -
  NGC85_RS12385 (NGC85_12385) - 2638424..2638747 (-) 324 WP_005057438.1 RnfH family protein -
  NGC85_RS12390 (NGC85_12390) - 2638747..2639829 (-) 1083 WP_254731575.1 hypothetical protein -
  NGC85_RS12395 (NGC85_12395) - 2640075..2640530 (-) 456 WP_169109971.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38329.96 Da        Isoelectric Point: 6.5054

>NTDB_id=708743 NGC85_RS12365 WP_254731571.1 2635149..2636186(+) (pilT) [Acinetobacter sp. Z1]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGMISPQTARNVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=708743 NGC85_RS12365 WP_254731571.1 2635149..2636186(+) (pilT) [Acinetobacter sp. Z1]
ATGGATATTACAGAATTACTCGCCTTTTCAGTGAAAAACGGTGCTTCAGATTTACACCTTTCAGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGCATTAACTTACCAGCATTAGAACATAAAGATGTACACCGTTTAGTGTACGACA
TTATGAATGACAAGCAACGTCGTGATTTCGAAGAAGACCTTGAAACAGACTTTTCATTTGAAGTTCCAAACATTGCCCGT
TTTCGTGTGAATGCATTCAACCAAAACCGTGGTGCTGGTGCAGTATTCCGTACCATTCCATCGAAAGTATTAACGATGGA
AGATTTAGGATTAGGATCAATTTTTAAAGAAATTTGTGACTATCCACGAGGTATCGTACTGGTCACAGGTCCTACTGGTT
CTGGTAAATCAACCACCTTGGCTGCAATGATCGACTATATTAATGAAAATCGTTATGACCATATTTTAACAGTCGAAGAT
CCAATCGAATTTGTACACCAATCTAAAAAGTGTTTGATCAACCAACGTGAAGTACATCGTGATACGCATGGCTTTAATGC
TGCATTACGTTCAGCATTGCGTGAAGATCCAGATATTATTTTGGTCGGTGAGATGCGTGACTTAGAGACTATTCGCCTTG
CCTTAACAGCTGCGGAAACAGGACACTTGGTTTTTGGTACGCTACACACCACCTCTGCTGCAAAAACTATTGACCGTGTT
ATCGACGTATTCCCTGCCGAAGAAAAAGATATGGTACGCGCCATGTTATCTGAATCTCTACAAGCAGTTATTTCGCAAAC
ACTGTTAAAGAAGAATGGTGGTGGGCGTGTAGCAGCACATGAAATCATGATCGGTATCCCTGCGATTCGTAACCTTGTTC
GTGAAAACAAAGTCGCACAAATGTACTCTGCTATTCAAACAGGTGCTAACTACGGTATGACCACGTTAGATCAAAGTCTC
AAAACTTTAGTATCTAAAGGTATGATCAGTCCCCAAACAGCGCGTAACGTTGCGAAACAACCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.942

100

0.959
  pilT Acinetobacter baumannii strain A118

95.652

100

0.957
  pilT Acinetobacter baumannii D1279779

95.652

100

0.957
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Pseudomonas stutzeri DSM 10701

82.849

99.71

0.826
  pilT Legionella pneumophila strain Lp02

75.872

99.71

0.757
  pilT Legionella pneumophila strain ERS1305867

75.872

99.71

0.757
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394