Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NGC85_RS12370 Genome accession   NZ_CP101115
Coordinates   2636211..2637329 (+) Length   372 a.a.
NCBI ID   WP_254731573.1    Uniprot ID   -
Organism   Acinetobacter sp. Z1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2631211..2642329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGC85_RS12345 (NGC85_12345) - 2632326..2632613 (+) 288 WP_254805600.1 hypothetical protein -
  NGC85_RS12350 (NGC85_12350) - 2632685..2633359 (+) 675 WP_254805601.1 HAD family hydrolase -
  NGC85_RS12355 (NGC85_12355) - 2633413..2634149 (-) 737 Protein_2412 hypothetical protein -
  NGC85_RS12360 (NGC85_12360) - 2634336..2635022 (-) 687 WP_254731569.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGC85_RS12365 (NGC85_12365) pilT 2635149..2636186 (+) 1038 WP_254731571.1 type IV pilus twitching motility protein PilT Machinery gene
  NGC85_RS12370 (NGC85_12370) pilU 2636211..2637329 (+) 1119 WP_254731573.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGC85_RS12375 (NGC85_12375) fur 2637424..2637861 (-) 438 WP_005056466.1 ferric iron uptake transcriptional regulator -
  NGC85_RS12380 (NGC85_12380) - 2637972..2638370 (+) 399 WP_005056468.1 outer membrane protein assembly factor BamE -
  NGC85_RS12385 (NGC85_12385) - 2638424..2638747 (-) 324 WP_005057438.1 RnfH family protein -
  NGC85_RS12390 (NGC85_12390) - 2638747..2639829 (-) 1083 WP_254731575.1 hypothetical protein -
  NGC85_RS12395 (NGC85_12395) - 2640075..2640530 (-) 456 WP_169109971.1 bacteriohemerythrin -
  NGC85_RS12400 (NGC85_12400) - 2640812..2641660 (-) 849 WP_254731577.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41747.88 Da        Isoelectric Point: 6.2887

>NTDB_id=708744 NGC85_RS12370 WP_254731573.1 2636211..2637329(+) (pilU) [Acinetobacter sp. Z1]
MDFNDLLNLMIQQNASDLFVTADVEPSMKINGQIVPVAKTKLSGEIVGQLVNSIMTDKQRKEFADTRECNFAITNREKTA
RFRVSAFQQRDMPGMILRKIETHIPTMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGFRNHNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLRAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGSDQLLNASKNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=708744 NGC85_RS12370 WP_254731573.1 2636211..2637329(+) (pilU) [Acinetobacter sp. Z1]
ATGGACTTTAATGATTTACTCAATTTGATGATTCAACAAAATGCATCGGACTTATTTGTGACTGCCGATGTAGAACCATC
AATGAAAATAAACGGGCAGATTGTGCCCGTTGCTAAAACCAAATTGTCAGGTGAAATTGTTGGTCAATTGGTCAATTCAA
TTATGACTGACAAGCAGCGTAAAGAGTTTGCTGACACGCGTGAATGTAACTTTGCAATTACCAACCGTGAAAAGACAGCT
CGATTCCGTGTCAGCGCTTTTCAACAACGTGATATGCCAGGCATGATCTTGCGTAAAATTGAAACGCATATTCCAACCAT
GGATGATCTTAAGTTACCCGAAGTTCTGAAAGAACTTGCAATGACTAAGCGCGGAATTATTATTTTCGTAGGTGCTACAG
GTACCGGTAAATCAACGTCATTGGCTTCAATGATCGGTTTCCGTAACCATAACTCGAAAGGTCATATCATTACCATTGAA
GACCCAATTGAGTTTGTACATCAACATGCAGGTTGTATCGTTACTCAGCGTGAAGTCGGTATTGATACAGACTCATTCGA
AATTGCGTTAAAAAATACATTACGTCAAGCGCCTGACGTGATCCTGATTGGTGAGATTCGTTCACGTGAAACCATGGACT
ATGCCATTGCATTCGCAGAAACTGGTCACTTGGTTTTAGCTACACTGCATGCAAATAACGCAAACCAAGCACTTGATCGT
ATTATTCATTTCTTTGAAGCCGACCGCCATAGTCAGCTCTACATGGATTTATCATTAAATTTAAGAGCAATGGTCGCCCA
ACAGCTTATCCCTACCCCAGATGGCAATTCACGCCGTGCGGCGATTGAAATTCTAATTAATACACCTTTATTGGCTGATT
ACATCCGTAAAGGTGAAGTACATGAAATTAAAGATCTGATGAAGCGTTCTCGTGAATTAGGAATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCAGGCGAAATTACATATAAAGATGCGTTGAAGCATGCTGACTCGCCAAATGACCTTCG
TCTCACGATCAAACTTTCAGAAGAAGGGTCTGATCAATTATTGAATGCCAGCAAAAATATTACCTTTGATGGTCAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.397

100

0.766
  pilU Pseudomonas stutzeri DSM 10701

70.081

99.731

0.699
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.659

97.849

0.398
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363
  pilT Legionella pneumophila strain ERS1305867

40.979

87.903

0.36
  pilT Legionella pneumophila strain Lp02

40.979

87.903

0.36
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36