Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD15_RS12360 Genome accession   NZ_CP101030
Coordinates   2745149..2746249 (-) Length   366 a.a.
NCBI ID   WP_010864554.1    Uniprot ID   R8AMI2
Organism   Plesiomonas shigelloides strain OT201     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2740149..2751249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD15_RS12330 (NMD15_12305) rsmE 2740610..2741344 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD15_RS12335 (NMD15_12310) gshB 2741354..2742307 (+) 954 WP_047708158.1 glutathione synthase -
  NMD15_RS12340 (NMD15_12315) - 2742521..2743084 (+) 564 WP_010864550.1 YqgE/AlgH family protein -
  NMD15_RS12345 (NMD15_12320) ruvX 2743109..2743528 (+) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  NMD15_RS12350 (NMD15_12325) npr 2743545..2743820 (-) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  NMD15_RS12355 (NMD15_12330) pilU 2744056..2745165 (-) 1110 WP_152137879.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMD15_RS12360 (NMD15_12335) pilT 2745149..2746249 (-) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD15_RS12365 (NMD15_12340) - 2746272..2746979 (+) 708 WP_039045867.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD15_RS12370 (NMD15_12345) proC 2747062..2747880 (+) 819 WP_370599046.1 pyrroline-5-carboxylate reductase -
  NMD15_RS12375 (NMD15_12350) - 2747909..2748463 (+) 555 WP_010864557.1 YggT family protein -
  NMD15_RS12380 (NMD15_12355) yggU 2748475..2748762 (+) 288 WP_152106355.1 DUF167 family protein YggU -
  NMD15_RS12385 (NMD15_12360) - 2748831..2749334 (+) 504 WP_370597974.1 DUF4426 domain-containing protein -
  NMD15_RS12390 (NMD15_12365) - 2749528..2750133 (+) 606 WP_370597975.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40421.07 Da        Isoelectric Point: 6.7432

>NTDB_id=708258 NMD15_RS12360 WP_010864554.1 2745149..2746249(-) (pilT) [Plesiomonas shigelloides strain OT201]
MDITELLAFSVKHNASDLHLSAGNPPLIRVDGEVRRVNMPVLDGREVRRLVYDIMNDVQRRDFETEMELDFSFELNGVGR
FRVNAYQQQRGISAALRTIPTDIPTLEQLNAPEVFRNIAMQPRGLVLVTGPTGSGKSTTLAAMLNYVNQHMHHHILTIED
PIEFIHTSNKCLINQREVHRHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPGAEKDMVRSMLSESLKAVISQVLLRKEGGGRVAAHEIMLGTPAIRNLIREDKIHQIYSILQTSSRQGMQTLEQAL
NVLTSQGVVSYEEAQRKMNSLMDGGIQNGNTAGGTGAGAMGYGFAG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=708258 NMD15_RS12360 WP_010864554.1 2745149..2746249(-) (pilT) [Plesiomonas shigelloides strain OT201]
ATGGACATAACAGAACTGTTGGCTTTTAGTGTAAAACATAATGCCTCGGATCTGCACCTATCTGCCGGCAACCCACCACT
GATCCGTGTCGACGGGGAAGTGCGTCGGGTCAATATGCCGGTCCTGGATGGCCGTGAAGTGCGCCGTCTGGTGTATGACA
TTATGAACGATGTTCAGCGCCGTGACTTTGAAACTGAGATGGAACTGGACTTTTCGTTCGAACTGAACGGGGTTGGCCGT
TTCCGGGTTAATGCTTATCAGCAGCAACGGGGAATTTCGGCGGCACTGCGTACCATACCTACAGATATACCAACACTGGA
GCAGTTAAACGCACCGGAAGTGTTTCGGAATATTGCCATGCAGCCGCGTGGACTGGTATTGGTGACCGGCCCGACCGGCT
CGGGGAAGTCGACCACCTTGGCGGCGATGCTCAATTATGTCAATCAGCACATGCATCACCACATTCTGACCATTGAAGAC
CCGATCGAATTTATCCACACCAGTAACAAGTGCCTGATTAACCAGCGTGAGGTGCATCGGCATACCCACAGTTTTAATAA
TGCACTGCGCTCGGCGCTGCGTGAAGACCCGGATGTGATTCTGGTCGGTGAGCTGCGCGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCTGAAACTGGCCACTTGGTGTTTGGCACCCTGCACACCAGCTCGGCGGCCAAAACCATTGACCGTATT
GTGGATGTGTTCCCCGGCGCAGAAAAAGACATGGTGCGCTCGATGCTGTCTGAATCGCTCAAAGCGGTTATCTCGCAGGT
ATTGCTACGTAAAGAAGGCGGCGGACGTGTGGCGGCGCACGAGATCATGCTGGGAACGCCAGCCATTCGTAACTTGATCC
GCGAAGATAAAATTCACCAGATCTATTCGATTTTGCAGACCAGCTCACGCCAAGGTATGCAGACGTTGGAGCAGGCACTG
AATGTGCTGACCAGTCAGGGCGTGGTCAGCTACGAAGAGGCGCAGCGCAAGATGAACTCGTTGATGGATGGCGGGATCCA
AAACGGCAATACCGCGGGTGGCACTGGTGCAGGAGCGATGGGCTATGGTTTCGCTGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8AMI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.373

92.35

0.678
  pilT Vibrio cholerae strain A1552

73.373

92.35

0.678
  pilT Pseudomonas stutzeri DSM 10701

71.217

92.077

0.656
  pilT Acinetobacter nosocomialis M2

71.343

91.53

0.653
  pilT Acinetobacter baumannii D1279779

71.045

91.53

0.65
  pilT Acinetobacter baumannii strain A118

71.045

91.53

0.65
  pilT Pseudomonas aeruginosa PAK

70.623

92.077

0.65
  pilT Acinetobacter baylyi ADP1

70.448

91.53

0.645
  pilT Neisseria meningitidis 8013

64.793

92.35

0.598
  pilT Neisseria gonorrhoeae MS11

64.497

92.35

0.596
  pilT Legionella pneumophila strain Lp02

65.269

91.257

0.596
  pilT Legionella pneumophila strain ERS1305867

65.269

91.257

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.025

98.087

0.481
  pilU Vibrio cholerae strain A1552

43.023

93.989

0.404
  pilU Pseudomonas stutzeri DSM 10701

43.284

91.53

0.396
  pilU Acinetobacter baylyi ADP1

42.274

93.716

0.396