Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMD15_RS12355 Genome accession   NZ_CP101030
Coordinates   2744056..2745165 (-) Length   369 a.a.
NCBI ID   WP_152137879.1    Uniprot ID   -
Organism   Plesiomonas shigelloides strain OT201     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2739056..2750165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD15_RS12320 (NMD15_12295) - 2739710..2739919 (-) 210 WP_010864545.1 DUF1656 domain-containing protein -
  NMD15_RS12325 (NMD15_12300) - 2739916..2740428 (-) 513 WP_010864546.1 MarR family winged helix-turn-helix transcriptional regulator -
  NMD15_RS12330 (NMD15_12305) rsmE 2740610..2741344 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD15_RS12335 (NMD15_12310) gshB 2741354..2742307 (+) 954 WP_047708158.1 glutathione synthase -
  NMD15_RS12340 (NMD15_12315) - 2742521..2743084 (+) 564 WP_010864550.1 YqgE/AlgH family protein -
  NMD15_RS12345 (NMD15_12320) ruvX 2743109..2743528 (+) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  NMD15_RS12350 (NMD15_12325) npr 2743545..2743820 (-) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  NMD15_RS12355 (NMD15_12330) pilU 2744056..2745165 (-) 1110 WP_152137879.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMD15_RS12360 (NMD15_12335) pilT 2745149..2746249 (-) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD15_RS12365 (NMD15_12340) - 2746272..2746979 (+) 708 WP_039045867.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD15_RS12370 (NMD15_12345) proC 2747062..2747880 (+) 819 WP_370599046.1 pyrroline-5-carboxylate reductase -
  NMD15_RS12375 (NMD15_12350) - 2747909..2748463 (+) 555 WP_010864557.1 YggT family protein -
  NMD15_RS12380 (NMD15_12355) yggU 2748475..2748762 (+) 288 WP_152106355.1 DUF167 family protein YggU -
  NMD15_RS12385 (NMD15_12360) - 2748831..2749334 (+) 504 WP_370597974.1 DUF4426 domain-containing protein -
  NMD15_RS12390 (NMD15_12365) - 2749528..2750133 (+) 606 WP_370597975.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41513.57 Da        Isoelectric Point: 5.7325

>NTDB_id=708257 NMD15_RS12355 WP_152137879.1 2744056..2745165(-) (pilU) [Plesiomonas shigelloides strain OT201]
MVSLDELLQEMLRQQASDLYVSVDVPAQLRINHELHPLGEVLSIETVTELVAQAMGAERFLQFCQQKEGNMALVRPFARF
RVSGFWQRELPGMVIRHIRTDIPTIEELGLPMLTGELAMTRKGLILVVGATGSGKTTTLAAMLGYRNEFDHSHILTVEDP
IEFIHPHKRCLITQREVGMDTESYEVALRNALRQAPDVIQVGEIRTRDTLQHAIGFSETGHLCIATLHANNASQALERMV
HMIPEGSREAFLFDLSNNLRAIIGQQLVPGITPGRRLLVHEILINTPRVTDLLRKNQLYELRDVMASNSEIGMCTFDQSL
YGLYRRGLISYTDALHYAESANDLRLRIRSSGDEPFSPNTFDDVTVDLS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=708257 NMD15_RS12355 WP_152137879.1 2744056..2745165(-) (pilU) [Plesiomonas shigelloides strain OT201]
ATGGTTTCGCTGGATGAGTTATTGCAGGAGATGCTGCGTCAGCAGGCGTCGGATCTGTATGTTTCGGTCGATGTGCCCGC
GCAGCTGCGCATCAATCATGAGCTGCATCCACTAGGTGAGGTGCTGTCGATTGAAACGGTCACCGAGCTGGTGGCGCAGG
CGATGGGCGCCGAGCGCTTCTTACAGTTTTGTCAGCAAAAAGAGGGCAACATGGCGCTGGTGCGTCCGTTTGCCCGTTTT
CGGGTCTCCGGTTTTTGGCAGCGTGAATTGCCGGGCATGGTGATCCGTCATATTCGAACCGATATTCCGACCATTGAAGA
GCTGGGCTTACCGATGTTAACCGGTGAGCTGGCGATGACGCGCAAAGGTTTAATTTTGGTGGTCGGCGCTACCGGCAGCG
GGAAAACCACTACACTGGCAGCGATGCTCGGCTATCGCAACGAGTTTGACCATTCGCATATTTTGACAGTGGAAGACCCG
ATTGAATTTATCCATCCGCATAAACGCTGTTTGATCACTCAGCGTGAAGTGGGGATGGATACCGAAAGCTATGAGGTAGC
CTTGCGTAACGCGCTGCGCCAAGCACCGGATGTGATTCAGGTTGGCGAGATCCGCACTCGTGACACGCTGCAACACGCCA
TTGGCTTTTCCGAAACTGGCCATCTGTGTATTGCCACCTTGCACGCCAATAACGCCAGCCAAGCGCTGGAGCGGATGGTA
CACATGATCCCTGAAGGCAGCCGCGAAGCTTTCTTGTTTGATTTATCCAACAACTTGCGCGCCATTATCGGGCAGCAGCT
GGTGCCGGGGATCACCCCAGGGCGCCGTTTACTGGTGCACGAGATTTTGATCAACACGCCGCGCGTGACCGATTTGCTGC
GTAAAAATCAGTTGTATGAGCTGCGTGATGTGATGGCCAGTAACAGCGAGATTGGCATGTGCACCTTTGACCAATCGCTG
TATGGCTTGTATCGGCGCGGGCTGATTAGCTATACCGATGCGCTGCACTATGCGGAGTCGGCCAATGATTTGCGTTTGCG
GATCCGCAGTAGCGGTGATGAGCCGTTCTCACCGAACACCTTCGATGATGTGACGGTGGATTTAAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

54.496

99.458

0.542
  pilU Pseudomonas stutzeri DSM 10701

51.275

95.664

0.491
  pilU Acinetobacter baylyi ADP1

46.742

95.664

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.344

99.187

0.39