Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OYT1_RS01205 Genome accession   NZ_AP018738
Coordinates   264899..265942 (+) Length   347 a.a.
NCBI ID   WP_062625718.1    Uniprot ID   A0A2Z6G8V6
Organism   Ferriphaselus amnicola strain OYT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259899..270942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OYT1_RS01170 (OYT1_ch0243) ftsL 260579..260869 (-) 291 WP_062625725.1 cell division protein FtsL -
  OYT1_RS01175 (OYT1_ch0244) rsmH 260866..261804 (-) 939 WP_062625724.1 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH -
  OYT1_RS01180 (OYT1_ch0245) mraZ 261801..262250 (-) 450 WP_062625723.1 division/cell wall cluster transcriptional repressor MraZ -
  OYT1_RS01185 (OYT1_ch0246) - 262465..262764 (-) 300 WP_062625722.1 DUF167 domain-containing protein -
  OYT1_RS01190 (OYT1_ch0247) - 262766..263332 (-) 567 WP_062625721.1 YggT family protein -
  OYT1_RS01195 (OYT1_ch0248) proC 263332..264141 (-) 810 WP_062625720.1 pyrroline-5-carboxylate reductase -
  OYT1_RS01200 (OYT1_ch0249) - 264138..264830 (-) 693 WP_062625719.1 YggS family pyridoxal phosphate-dependent enzyme -
  OYT1_RS01205 (OYT1_ch0250) pilT 264899..265942 (+) 1044 WP_062625718.1 type IV pilus twitching motility protein PilT Machinery gene
  OYT1_RS01210 (OYT1_ch0251) pilU 265962..267098 (+) 1137 WP_062625717.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OYT1_RS01215 (OYT1_ch0252) - 267101..268246 (-) 1146 WP_062625716.1 class I SAM-dependent RNA methyltransferase -
  OYT1_RS01220 (OYT1_ch0253) - 268248..268658 (-) 411 WP_062625715.1 CopD family protein -
  OYT1_RS01225 (OYT1_ch0254) - 268815..269006 (-) 192 WP_062625714.1 hypothetical protein -
  OYT1_RS01230 (OYT1_ch0255) - 269006..269839 (-) 834 WP_062625713.1 cytochrome c3 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38268.28 Da        Isoelectric Point: 7.1072

>NTDB_id=70617 OYT1_RS01205 WP_062625718.1 264899..265942(+) (pilT) [Ferriphaselus amnicola strain OYT1]
MDITELLAFGVKSKASDLHLSAGLPPMIRVHGDIRRINLPPLESKDVHAMVYDIMNDGQRKHYEENKEIDFSFEVPGLAR
FRVNAFNHQRGVGAVLRTIPSTVLTLEDLKAPKIFKDISEYPRGIVLVTGPTGSGKSTTLAAMINHVNETMLGHILTVED
PIEFVHESKKCLINQREVGPHTISFSNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFATLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQTLLKTKDGAGRVAAHEIMICTPAIRNLIREAKVPQMYSAIQTGQAIGMQTLDQC
LMDLVRRGVVASSEARTRAANKDMFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=70617 OYT1_RS01205 WP_062625718.1 264899..265942(+) (pilT) [Ferriphaselus amnicola strain OYT1]
GTGGATATTACCGAACTGCTCGCCTTTGGCGTCAAGAGTAAAGCTTCAGATTTACACCTTTCTGCCGGCTTGCCACCGAT
GATACGCGTGCATGGTGACATCCGCCGCATCAACCTTCCGCCGCTGGAAAGCAAAGATGTACACGCTATGGTGTACGACA
TCATGAACGACGGACAGCGCAAGCATTACGAGGAAAACAAGGAGATCGATTTCTCTTTTGAGGTGCCGGGACTGGCACGT
TTTCGCGTCAATGCTTTCAATCATCAACGTGGCGTGGGTGCGGTACTGCGGACCATTCCTTCCACGGTACTGACGCTGGA
AGATCTGAAAGCGCCCAAGATATTCAAGGACATCTCAGAGTACCCGCGCGGTATCGTGCTGGTGACTGGGCCGACGGGCT
CAGGTAAGTCCACTACCTTGGCGGCGATGATCAATCATGTCAACGAGACCATGCTGGGACACATCCTTACGGTGGAAGAC
CCGATCGAGTTCGTGCATGAGAGCAAAAAGTGTCTGATCAATCAGCGGGAAGTCGGCCCTCACACGATCTCATTCAGTAA
TGCGCTACGTTCGGCGTTGCGTGAAGACCCTGACGTGATTCTGGTCGGTGAAATGCGCGATCTGGAAACCATCCGTCTGG
CGATGACCGCCGCCGAAACCGGACACTTGGTGTTCGCCACGCTGCACACCAGCTCTGCCGCCAAGACCATCGACCGTATC
ATCGATGTGTTCCCCGCCGACGAGAAGGAAATGGTGCGCGCCATGTTGTCGGAATCGTTGCGTGCCGTCATCTCTCAAAC
TTTGCTCAAGACCAAGGACGGTGCAGGTCGCGTAGCGGCACACGAGATCATGATCTGTACTCCGGCGATTCGAAATCTGA
TCCGCGAAGCTAAAGTGCCGCAGATGTACTCCGCGATCCAGACCGGCCAAGCCATCGGTATGCAGACGCTGGATCAGTGC
CTGATGGATTTGGTGCGGCGCGGTGTCGTCGCTTCATCCGAGGCTCGCACCCGTGCCGCCAACAAGGATATGTTCCCGGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6G8V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Pseudomonas stutzeri DSM 10701

72.754

99.424

0.723
  pilT Neisseria meningitidis 8013

72.303

98.847

0.715
  pilT Neisseria gonorrhoeae MS11

72.012

98.847

0.712
  pilT Legionella pneumophila strain Lp02

70.725

99.424

0.703
  pilT Legionella pneumophila strain ERS1305867

70.725

99.424

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.406

0.657
  pilT Vibrio cholerae strain A1552

67.456

97.406

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

41.471

97.983

0.406
  pilU Vibrio cholerae strain A1552

42.769

93.66

0.401

Multiple sequence alignment