Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OYT1_RS01210 Genome accession   NZ_AP018738
Coordinates   265962..267098 (+) Length   378 a.a.
NCBI ID   WP_062625717.1    Uniprot ID   A0A2Z6G8V8
Organism   Ferriphaselus amnicola strain OYT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 260962..272098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OYT1_RS01180 (OYT1_ch0245) mraZ 261801..262250 (-) 450 WP_062625723.1 division/cell wall cluster transcriptional repressor MraZ -
  OYT1_RS01185 (OYT1_ch0246) - 262465..262764 (-) 300 WP_062625722.1 DUF167 domain-containing protein -
  OYT1_RS01190 (OYT1_ch0247) - 262766..263332 (-) 567 WP_062625721.1 YggT family protein -
  OYT1_RS01195 (OYT1_ch0248) proC 263332..264141 (-) 810 WP_062625720.1 pyrroline-5-carboxylate reductase -
  OYT1_RS01200 (OYT1_ch0249) - 264138..264830 (-) 693 WP_062625719.1 YggS family pyridoxal phosphate-dependent enzyme -
  OYT1_RS01205 (OYT1_ch0250) pilT 264899..265942 (+) 1044 WP_062625718.1 type IV pilus twitching motility protein PilT Machinery gene
  OYT1_RS01210 (OYT1_ch0251) pilU 265962..267098 (+) 1137 WP_062625717.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OYT1_RS01215 (OYT1_ch0252) - 267101..268246 (-) 1146 WP_062625716.1 class I SAM-dependent RNA methyltransferase -
  OYT1_RS01220 (OYT1_ch0253) - 268248..268658 (-) 411 WP_062625715.1 CopD family protein -
  OYT1_RS01225 (OYT1_ch0254) - 268815..269006 (-) 192 WP_062625714.1 hypothetical protein -
  OYT1_RS01230 (OYT1_ch0255) - 269006..269839 (-) 834 WP_062625713.1 cytochrome c3 family protein -
  OYT1_RS01235 (OYT1_ch0256) nrfD 269937..271184 (-) 1248 WP_062625712.1 NrfD/PsrC family molybdoenzyme membrane anchor subunit -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41902.48 Da        Isoelectric Point: 6.2326

>NTDB_id=70618 OYT1_RS01210 WP_062625717.1 265962..267098(+) (pilU) [Ferriphaselus amnicola strain OYT1]
MDRVLATELIHSLLRQMLARKASDLFITAGFPPALKIDGVLTPLLSPPLSAADTQDMANCLMSPRQSDAFETTHECNFAI
SPPEIGRFRVNVFLQQMNVGMVLRTITTQIPSVESLNLPPILKDIVMSKRGLVILVGATGSGKSTTLAAMVGHRNEFSHG
HIVTVEDPVEFVHEHRNCIVTHREIGIDTESWLSALKNAMRQAPDVILIGEIRDREAMEYAISFAETGHLCLATLHANNA
DQAIDRIVSFYPEERHEQVLMSLSLNLKSLISQRLVPRKNSTGRIAAMEILLNSPLIADLIFKGEINEIKAVMSKSRELG
MQTFDQALFTLFDSDLITYEEALKNADSLNDLRLRIKLESKAAKDNDLMSGIGHLKLT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=70618 OYT1_RS01210 WP_062625717.1 265962..267098(+) (pilU) [Ferriphaselus amnicola strain OYT1]
ATGGATCGCGTACTTGCGACTGAACTGATTCACTCTCTGTTGCGCCAGATGTTGGCGCGCAAGGCTTCCGATTTATTCAT
TACTGCTGGTTTTCCGCCTGCGCTGAAGATCGACGGGGTGCTGACACCGCTTCTGAGCCCGCCGCTGAGTGCTGCGGATA
CTCAGGATATGGCGAACTGCCTGATGAGCCCTAGGCAGAGTGATGCTTTCGAGACGACGCACGAGTGTAATTTCGCTATC
AGTCCGCCGGAGATCGGTCGTTTCCGGGTCAATGTCTTTTTGCAGCAGATGAATGTCGGCATGGTGCTGCGCACCATCAC
CACACAGATTCCCAGTGTGGAAAGCCTGAATTTGCCGCCTATCCTCAAAGACATCGTGATGAGTAAGCGTGGACTGGTGA
TTTTGGTTGGCGCCACCGGCTCGGGTAAATCCACCACACTGGCCGCGATGGTTGGTCATCGCAACGAGTTCAGTCACGGC
CATATCGTTACCGTGGAAGACCCCGTTGAATTTGTCCATGAGCATCGCAATTGCATCGTCACCCATCGTGAGATCGGCAT
AGATACGGAATCTTGGCTGTCCGCCCTCAAGAATGCCATGCGTCAAGCACCTGATGTCATTCTGATCGGTGAGATACGTG
ACCGCGAGGCGATGGAATACGCTATCTCCTTCGCCGAGACCGGCCATCTGTGTTTGGCTACCTTGCACGCCAACAATGCC
GACCAAGCGATTGATCGCATCGTCAGCTTTTACCCGGAGGAGCGGCATGAACAGGTGTTAATGAGTTTGTCGCTCAATTT
GAAGAGTCTGATCTCGCAGAGGCTGGTGCCACGCAAGAACAGCACGGGTCGTATCGCCGCGATGGAAATCCTACTGAACT
CGCCGCTCATCGCCGACCTCATCTTCAAGGGGGAGATCAATGAGATCAAAGCGGTGATGAGCAAATCACGTGAGCTGGGG
ATGCAGACGTTCGATCAGGCGTTATTCACTTTGTTTGATAGCGACCTGATCACCTATGAAGAAGCGTTGAAGAATGCCGA
TTCGCTCAACGATCTGCGCCTGCGCATCAAGCTGGAAAGCAAGGCGGCTAAGGACAATGACCTGATGTCGGGCATTGGCC
ATTTGAAGCTGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6G8V8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.453

92.328

0.54
  pilU Acinetobacter baylyi ADP1

56.941

93.386

0.532
  pilU Vibrio cholerae strain A1552

54.42

95.767

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.276

97.619

0.413
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365

Multiple sequence alignment