Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL386_RS01475 Genome accession   NZ_AP018725
Coordinates   293028..294065 (+) Length   345 a.a.
NCBI ID   WP_126452580.1    Uniprot ID   -
Organism   Sulfuriflexus mobilis strain aks1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 288028..299065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL386_RS01455 - 290158..290451 (-) 294 WP_126452572.1 DUF167 family protein -
  EL386_RS01460 - 290460..291077 (-) 618 WP_172597574.1 YggT family protein -
  EL386_RS01465 proC 291077..291904 (-) 828 WP_126452576.1 pyrroline-5-carboxylate reductase -
  EL386_RS01470 - 292211..292897 (-) 687 WP_126452578.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL386_RS01475 pilT 293028..294065 (+) 1038 WP_126452580.1 type IV pilus twitching motility protein PilT Machinery gene
  EL386_RS01480 pilU 294110..295261 (+) 1152 WP_126452582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL386_RS01485 - 295341..295757 (-) 417 WP_126452584.1 MarR family winged helix-turn-helix transcriptional regulator -
  EL386_RS01490 - 295912..296481 (+) 570 WP_172597575.1 DsrE/DsrF/DrsH-like family protein -
  EL386_RS01495 - 296640..297137 (-) 498 WP_126452588.1 EamA family transporter -
  EL386_RS01500 - 297233..298201 (-) 969 WP_126452590.1 calcium/sodium antiporter -
  EL386_RS01505 - 298299..298721 (-) 423 WP_126452592.1 thioesterase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38140.80 Da        Isoelectric Point: 6.5059

>NTDB_id=70533 EL386_RS01475 WP_126452580.1 293028..294065(+) (pilT) [Sulfuriflexus mobilis strain aks1]
MDIAELLAFGVKNNASDLHLSAGLPPMIRVDGDIRRINVPALDHKQVHSLIYDIMNDKQRKDFEEFFETDFSFEIPGLAR
FRVNAFNHNRGAGAVFRTIPSRILSLEELGAPAVFKEISDVPRGIVLVTGPTGSGKSTTLAAMVDYKNDNEFGHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVIAQTLLKKIGGGRIAAHEIMIGNPAIRNLIRENKVAQMYSAIQTGQAVGMQTLDQCL
KDLVAKGQITVQDARMRAVNKEQFS

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=70533 EL386_RS01475 WP_126452580.1 293028..294065(+) (pilT) [Sulfuriflexus mobilis strain aks1]
ATGGATATTGCTGAACTACTTGCCTTCGGTGTGAAGAACAACGCCTCGGACCTGCACCTGTCCGCCGGCCTGCCACCGAT
GATTCGTGTCGATGGTGACATTCGTCGTATTAACGTGCCAGCACTGGATCATAAGCAAGTACACAGTCTCATTTACGACA
TCATGAACGACAAACAGCGTAAGGACTTCGAAGAATTTTTCGAGACCGACTTCTCGTTTGAAATTCCAGGCCTGGCGCGT
TTCCGCGTCAATGCCTTTAACCATAACCGTGGCGCGGGTGCGGTGTTTCGTACCATTCCCTCGCGTATCCTGTCGCTGGA
AGAACTCGGTGCGCCAGCGGTGTTTAAGGAGATCTCCGATGTGCCGCGTGGCATTGTGCTCGTCACCGGCCCGACCGGTT
CCGGCAAGTCGACCACACTGGCCGCCATGGTCGACTACAAGAACGATAACGAGTTTGGGCACATTCTTACGGTTGAAGAC
CCGATCGAATTTGTTCACGAGAGTAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGATACACTGGGTTTTAATGA
GGCGCTACGTTCGGCCTTGCGTGAAGACCCGGATATTATCCTCGTTGGCGAAATGCGTGACCTGGAAACTATTCGCCTGG
CCCTGTCTGCGGCGGAAACCGGGCACCTTGTATTCGGTACCCTGCATACCAGTTCTGCCGCCAAGACCATCGACCGTATT
ATCGACGTGTTCCCGGCCGCAGAAAAAGATATGGTGCGTTCGATGTTGTCAGAATCCCTGCGCGCAGTTATTGCCCAGAC
CCTGCTGAAAAAGATTGGCGGTGGCCGTATTGCTGCGCACGAGATCATGATCGGCAATCCGGCGATTCGTAACCTCATTC
GAGAAAACAAGGTCGCGCAGATGTACTCCGCCATCCAGACCGGCCAGGCCGTCGGCATGCAGACCCTCGATCAGTGTCTG
AAAGACCTGGTCGCCAAGGGTCAGATCACGGTACAGGATGCACGTATGCGTGCGGTAAACAAGGAACAGTTTTCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.977

99.71

0.817
  pilT Pseudomonas stutzeri DSM 10701

80.814

99.71

0.806
  pilT Acinetobacter baumannii D1279779

80.523

99.71

0.803
  pilT Acinetobacter baumannii strain A118

80.523

99.71

0.803
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baylyi ADP1

79.651

99.71

0.794
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.681

0.681
  pilT Vibrio cholerae strain A1552

69.733

97.681

0.681
  pilT Neisseria meningitidis 8013

67.341

100

0.675
  pilT Neisseria gonorrhoeae MS11

67.052

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.133

100

0.493
  pilU Vibrio cholerae strain A1552

42.985

97.101

0.417
  pilU Pseudomonas stutzeri DSM 10701

41.888

98.261

0.412
  pilU Acinetobacter baylyi ADP1

40.774

97.391

0.397

Multiple sequence alignment