Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL386_RS01480 Genome accession   NZ_AP018725
Coordinates   294110..295261 (+) Length   383 a.a.
NCBI ID   WP_126452582.1    Uniprot ID   -
Organism   Sulfuriflexus mobilis strain aks1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 289110..300261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL386_RS01455 - 290158..290451 (-) 294 WP_126452572.1 DUF167 family protein -
  EL386_RS01460 - 290460..291077 (-) 618 WP_172597574.1 YggT family protein -
  EL386_RS01465 proC 291077..291904 (-) 828 WP_126452576.1 pyrroline-5-carboxylate reductase -
  EL386_RS01470 - 292211..292897 (-) 687 WP_126452578.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL386_RS01475 pilT 293028..294065 (+) 1038 WP_126452580.1 type IV pilus twitching motility protein PilT Machinery gene
  EL386_RS01480 pilU 294110..295261 (+) 1152 WP_126452582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL386_RS01485 - 295341..295757 (-) 417 WP_126452584.1 MarR family winged helix-turn-helix transcriptional regulator -
  EL386_RS01490 - 295912..296481 (+) 570 WP_172597575.1 DsrE/DsrF/DrsH-like family protein -
  EL386_RS01495 - 296640..297137 (-) 498 WP_126452588.1 EamA family transporter -
  EL386_RS01500 - 297233..298201 (-) 969 WP_126452590.1 calcium/sodium antiporter -
  EL386_RS01505 - 298299..298721 (-) 423 WP_126452592.1 thioesterase family protein -
  EL386_RS01510 - 298774..299277 (-) 504 WP_126452594.1 GrpB family protein -
  EL386_RS01515 - 299466..299747 (-) 282 WP_126452596.1 putative quinol monooxygenase -
  EL386_RS01520 - 299823..300194 (-) 372 WP_126452598.1 hypothetical protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42081.39 Da        Isoelectric Point: 6.1369

>NTDB_id=70534 EL386_RS01480 WP_126452582.1 294110..295261(+) (pilU) [Sulfuriflexus mobilis strain aks1]
MDFDSLLKLMVQKNASDLFITAGMAPSLKVNGKITPVTKNTLTPGQAMEVVEGIMSEAQKKEFHDTHECNFAISASGIGR
FRVSAFIQRNHAGMVLRKIETKIPSLEELRLPAIIKNLAMVKRGLVLFVGGTGTGKSTSLAAMIGYRNKNTTGHIISIED
PIEYIHQHDGCIITQREVGLDTDSFETALKNTLRQAPDVILIGEIRTRETMEHAITFAETGHLCLATLHANNANQALDRI
INFFPEDRRNQLLMDLSLNLKGIIAQQLIPTPDGKGRRAAIEVLINTPLAADIIRKGAVHELKELMRKSNQLGMRTFDQA
LYELYTAGEITYEDAIHAADSANELRLMIKLGDKAAAENMESAVEGMTLMEEDDNSPGGGLSF

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=70534 EL386_RS01480 WP_126452582.1 294110..295261(+) (pilU) [Sulfuriflexus mobilis strain aks1]
ATGGATTTTGATTCGCTGCTCAAACTCATGGTACAGAAAAACGCATCTGACCTGTTCATCACCGCCGGTATGGCCCCGTC
GCTGAAGGTCAACGGCAAGATCACGCCGGTGACCAAGAACACCTTGACCCCGGGCCAGGCCATGGAAGTGGTAGAGGGGA
TCATGAGTGAGGCACAAAAGAAAGAGTTTCACGATACCCATGAGTGTAATTTCGCGATCTCGGCCTCCGGTATAGGACGC
TTCCGCGTCAGTGCCTTTATCCAGCGTAACCATGCCGGCATGGTACTGCGAAAGATCGAAACCAAGATCCCTTCACTGGA
AGAGCTGCGTCTGCCGGCGATCATCAAGAACCTCGCCATGGTCAAGCGTGGTTTGGTATTGTTCGTCGGTGGTACGGGCA
CCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGTAACAAGAACACGACCGGGCATATCATCAGTATCGAAGAT
CCGATCGAATACATTCATCAGCATGATGGCTGCATCATCACCCAGCGTGAAGTCGGCCTGGATACCGATTCATTTGAAAC
GGCACTGAAGAATACCCTGCGTCAGGCACCGGATGTCATCCTCATTGGTGAGATCCGGACCCGCGAAACGATGGAGCACG
CGATCACCTTCGCCGAGACCGGTCACCTCTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGTATT
ATTAACTTCTTCCCGGAAGACCGTCGTAACCAGTTGCTCATGGACCTGTCACTGAACCTGAAGGGTATTATTGCCCAGCA
ATTGATCCCGACCCCGGATGGCAAGGGCCGTCGCGCTGCGATCGAGGTGCTGATCAATACACCGCTTGCCGCCGACATTA
TTCGCAAGGGTGCGGTACACGAATTGAAGGAACTCATGCGCAAGTCCAACCAGCTGGGTATGAGGACCTTCGACCAGGCG
TTGTATGAACTGTATACGGCCGGTGAAATCACTTACGAAGATGCCATTCACGCTGCCGACTCAGCCAACGAACTGCGTTT
GATGATCAAGCTCGGCGACAAGGCGGCTGCCGAAAACATGGAATCGGCCGTTGAGGGTATGACCCTGATGGAAGAAGATG
ACAATAGCCCCGGGGGCGGGCTGTCATTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

73.81

98.695

0.728
  pilU Acinetobacter baylyi ADP1

64.326

92.95

0.598
  pilU Vibrio cholerae strain A1552

57.143

91.384

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.776

93.211

0.371

Multiple sequence alignment