Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EP379_RS11035 Genome accession   NZ_AP018724
Coordinates   2177718..2178146 (+) Length   142 a.a.
NCBI ID   WP_127477860.1    Uniprot ID   -
Organism   Sulfurivermis fontis strain JG42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2172718..2183146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP379_RS11010 - 2173391..2173621 (+) 231 WP_127477855.1 PP0621 family protein -
  EP379_RS11015 - 2173625..2175247 (+) 1623 WP_127477856.1 sensor histidine kinase -
  EP379_RS11020 pilR 2175244..2176599 (+) 1356 WP_127477857.1 sigma-54-dependent transcriptional regulator Regulator
  EP379_RS11025 - 2176679..2176906 (+) 228 WP_127477858.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  EP379_RS11030 - 2176896..2177336 (+) 441 WP_127477859.1 type II toxin-antitoxin system VapC family toxin -
  EP379_RS11035 pilA 2177718..2178146 (+) 429 WP_127477860.1 pilin Machinery gene
  EP379_RS11040 - 2178276..2178707 (+) 432 WP_127477861.1 pilin -
  EP379_RS11045 pilB 2178885..2180600 (+) 1716 WP_172600452.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EP379_RS11050 pilC 2180603..2181823 (+) 1221 WP_127477862.1 type II secretion system F family protein Machinery gene
  EP379_RS11055 pilD 2181837..2182709 (+) 873 WP_197722776.1 prepilin peptidase Machinery gene

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14465.49 Da        Isoelectric Point: 4.6320

>NTDB_id=70520 EP379_RS11035 WP_127477860.1 2177718..2178146(+) (pilA) [Sulfurivermis fontis strain JG42]
MKKQAGFTLIELMIVVAIIGILAAIAIPQYQDYIARTQVNRAYGEVSALRTAVEENLMRGNAGFAAADLGYVASNITTGA
LTINFAATGAGSLAVTLGGNAAAAVAGTTITLNRAVDGTWTCDVDESGAGSWKASYMPAGCS

Nucleotide


Download         Length: 429 bp        

>NTDB_id=70520 EP379_RS11035 WP_127477860.1 2177718..2178146(+) (pilA) [Sulfurivermis fontis strain JG42]
ATGAAGAAGCAAGCCGGCTTTACCCTTATCGAACTGATGATCGTGGTTGCCATCATCGGCATCCTGGCCGCCATCGCCAT
TCCGCAGTACCAGGACTACATTGCCCGTACCCAGGTCAACCGCGCCTACGGTGAGGTTTCTGCCCTGCGCACTGCGGTGG
AAGAGAACCTGATGCGCGGCAACGCCGGCTTCGCTGCCGCTGACCTTGGCTATGTCGCCTCCAACATCACTACCGGTGCA
CTGACCATCAACTTCGCGGCCACCGGCGCGGGCTCCCTGGCCGTCACGCTGGGCGGCAATGCCGCCGCGGCCGTCGCCGG
TACGACCATCACCCTGAACCGCGCCGTTGATGGCACCTGGACCTGTGATGTGGACGAGAGCGGCGCCGGTAGCTGGAAGG
CCAGCTACATGCCCGCCGGTTGTTCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.154

100

0.507
  pilA Acinetobacter baumannii strain A118

44.828

100

0.458
  pilA2 Legionella pneumophila str. Paris

44.755

100

0.451
  pilA2 Legionella pneumophila strain ERS1305867

44.755

100

0.451
  pilA Ralstonia pseudosolanacearum GMI1000

37.195

100

0.43
  comP Acinetobacter baylyi ADP1

38.217

100

0.423
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.714

98.592

0.401
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.748

100

0.401
  pilA Vibrio cholerae C6706

37.748

100

0.401
  pilA Vibrio cholerae strain A1552

37.748

100

0.401
  pilA/pilA1 Eikenella corrodens VA1

39.568

97.887

0.387
  pilA Haemophilus influenzae 86-028NP

41.6

88.028

0.366
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.956

96.479

0.366

Multiple sequence alignment