Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EP379_RS11045 Genome accession   NZ_AP018724
Coordinates   2178885..2180600 (+) Length   571 a.a.
NCBI ID   WP_172600452.1    Uniprot ID   -
Organism   Sulfurivermis fontis strain JG42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2173885..2185600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP379_RS11020 pilR 2175244..2176599 (+) 1356 WP_127477857.1 sigma-54-dependent transcriptional regulator Regulator
  EP379_RS11025 - 2176679..2176906 (+) 228 WP_127477858.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  EP379_RS11030 - 2176896..2177336 (+) 441 WP_127477859.1 type II toxin-antitoxin system VapC family toxin -
  EP379_RS11035 pilA 2177718..2178146 (+) 429 WP_127477860.1 pilin Machinery gene
  EP379_RS11040 - 2178276..2178707 (+) 432 WP_127477861.1 pilin -
  EP379_RS11045 pilB 2178885..2180600 (+) 1716 WP_172600452.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EP379_RS11050 pilC 2180603..2181823 (+) 1221 WP_127477862.1 type II secretion system F family protein Machinery gene
  EP379_RS11055 pilD 2181837..2182709 (+) 873 WP_197722776.1 prepilin peptidase Machinery gene
  EP379_RS11060 coaE 2182713..2183312 (+) 600 WP_127477863.1 dephospho-CoA kinase -
  EP379_RS11065 zapD 2183343..2184110 (+) 768 WP_127477864.1 cell division protein ZapD -
  EP379_RS11070 yacG 2184107..2184307 (+) 201 WP_127477865.1 DNA gyrase inhibitor YacG -
  EP379_RS11075 - 2184319..2185266 (-) 948 WP_127477866.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62349.81 Da        Isoelectric Point: 5.6245

>NTDB_id=70521 EP379_RS11045 WP_172600452.1 2178885..2180600(+) (pilB) [Sulfurivermis fontis strain JG42]
MATSGLRVQLSGLARRLIRDGLLSEADAEKATEQANKKRTPLVSYLVEQKLVEARAIAMATAHEFGVPLLDITVFDLDPE
LIKQVDEKLIRNHHALPIFKRGSRLFVALSDPTNIAALDEFKFHVGANTEAILVEEDKLTKVIEKALESKESALSDIADT
DLDDLEISAEDEEGGGPEVTASDADDTPVVRFINKVLLDAVNKGASDIHFEPYEKMYRVRFRQDGILREVANPPTALSAK
IAARIKVMSRLDISERRVPQDGRIKMTLSKNRAIDFRVSTCPTLWGEKIVLRILDPSSAKLGIDVLGYEPEQKELYLSAL
AKPYGMILVTGPTGSGKTVSLYTGLNILNTPDRNISTAEDPAEINLPGINQVNVNPKVGLTFAAALKAFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLARLMNIGVAPYNIAAAVNLIIAQRLARRLCNHCKKPEDVPKEALIA
EGFTEAQLAGLTIYKPVGCDQCNGGYKGRVGIYQVMPVSEEMGRIIMKGGTAMDLADQAKREGIADLRESAIKKLRAGVI
GLEELNRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=70521 EP379_RS11045 WP_172600452.1 2178885..2180600(+) (pilB) [Sulfurivermis fontis strain JG42]
ATGGCGACATCAGGATTGCGTGTACAACTGAGCGGACTGGCTCGCCGCTTAATTCGGGACGGACTCCTGAGCGAGGCCGA
TGCGGAGAAGGCCACCGAACAGGCCAACAAAAAGCGCACTCCGTTGGTCAGCTATCTGGTGGAGCAAAAGCTGGTCGAAG
CCCGTGCCATCGCCATGGCGACGGCCCATGAATTCGGCGTCCCCCTGCTCGACATCACCGTATTCGACCTCGATCCGGAA
CTCATCAAGCAGGTCGATGAAAAGCTGATTCGCAATCACCACGCCCTGCCCATCTTCAAGCGCGGCAGCCGCCTGTTCGT
TGCCCTGTCCGATCCGACCAACATCGCTGCACTCGACGAATTCAAATTTCACGTCGGCGCCAATACCGAAGCCATCCTCG
TCGAGGAGGACAAGCTCACCAAGGTCATCGAGAAAGCGCTGGAAAGCAAGGAAAGCGCACTGTCGGATATTGCCGACACC
GACCTCGATGACCTGGAGATCAGCGCTGAGGACGAGGAGGGCGGCGGCCCCGAGGTTACCGCCTCCGATGCCGATGACAC
GCCGGTGGTACGCTTCATCAACAAGGTATTGCTCGATGCCGTCAACAAGGGCGCCTCGGATATCCACTTCGAGCCATACG
AAAAGATGTACCGTGTCCGCTTCCGCCAGGATGGCATCCTGCGCGAGGTGGCCAACCCGCCGACCGCGTTGAGCGCCAAG
ATAGCCGCCCGTATCAAGGTCATGTCGCGCCTGGACATCTCCGAGCGCCGCGTGCCCCAGGACGGCCGTATCAAGATGAC
ACTGTCGAAGAACCGCGCCATCGACTTCCGTGTCAGCACCTGTCCCACCCTGTGGGGCGAAAAGATCGTGTTGCGTATCC
TCGATCCCTCCAGCGCCAAGCTGGGCATCGACGTGCTGGGTTACGAGCCGGAACAGAAAGAGCTCTATTTGAGCGCCCTG
GCCAAGCCCTACGGCATGATCCTGGTTACCGGCCCGACCGGTTCCGGTAAAACCGTATCGCTGTATACCGGCCTCAACAT
CCTCAATACACCGGATCGCAATATCTCCACCGCGGAAGACCCGGCGGAAATCAATCTACCCGGCATCAATCAGGTCAATG
TCAATCCCAAGGTCGGACTCACCTTCGCCGCCGCCCTGAAGGCCTTCCTGCGTCAGGATCCCGACGTCATCATGGTGGGT
GAAATTCGTGACCTGGAAACGGCGGAAATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTACTGTCCACCCTGCACAC
CAATGACGCCCCGCAGACGCTGGCGCGACTGATGAACATCGGTGTGGCTCCCTACAACATTGCTGCCGCGGTCAACCTCA
TCATCGCGCAGCGCCTGGCTCGACGTCTGTGCAACCACTGCAAGAAACCGGAGGATGTACCCAAGGAAGCGCTGATTGCC
GAGGGCTTCACCGAGGCGCAGCTCGCCGGCCTCACCATCTACAAACCGGTCGGCTGTGATCAGTGCAATGGCGGCTACAA
GGGACGCGTCGGTATCTATCAGGTCATGCCAGTATCGGAAGAAATGGGCCGCATCATCATGAAAGGCGGCACGGCGATGG
ACCTTGCCGACCAGGCCAAGCGCGAGGGCATCGCCGATCTGCGTGAATCGGCCATCAAGAAACTGCGCGCCGGCGTCATC
GGACTGGAAGAACTCAACCGTGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56

100

0.564
  pilB Acinetobacter baumannii D1279779

55.986

99.475

0.557
  pilB Acinetobacter baylyi ADP1

55.752

98.949

0.552
  pilF Neisseria gonorrhoeae MS11

51.056

99.475

0.508
  pilB Vibrio cholerae strain A1552

51.418

98.774

0.508
  pilB Vibrio parahaemolyticus RIMD 2210633

49.561

99.65

0.494
  pilB Vibrio campbellii strain DS40M4

48.85

98.949

0.483
  pilF Thermus thermophilus HB27

40.86

97.723

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.931

96.497

0.366

Multiple sequence alignment