Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L4174_RS02425 Genome accession   NZ_CP100425
Coordinates   521815..522849 (+) Length   344 a.a.
NCBI ID   WP_248143985.1    Uniprot ID   -
Organism   Photobacterium sp. CCB-ST2H9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516815..527849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L4174_RS02390 (L4174_002390) hemW 516883..518058 (-) 1176 WP_248143978.1 radical SAM family heme chaperone HemW -
  L4174_RS02395 (L4174_002395) - 518058..518651 (-) 594 WP_248143979.1 XTP/dITP diphosphatase -
  L4174_RS02400 (L4174_002400) - 518806..519234 (-) 429 WP_248143980.1 DUF4426 domain-containing protein -
  L4174_RS02405 (L4174_002405) yggU 519359..519652 (-) 294 WP_248143981.1 DUF167 family protein YggU -
  L4174_RS02410 (L4174_002410) - 519649..520206 (-) 558 WP_248143982.1 YggT family protein -
  L4174_RS02415 (L4174_002415) proC 520216..521034 (-) 819 WP_248143983.1 pyrroline-5-carboxylate reductase -
  L4174_RS02420 (L4174_002420) - 521084..521791 (-) 708 WP_248143984.1 YggS family pyridoxal phosphate-dependent enzyme -
  L4174_RS02425 (L4174_002425) pilT 521815..522849 (+) 1035 WP_248143985.1 type IV pilus twitching motility protein PilT Machinery gene
  L4174_RS02430 (L4174_002430) pilU 522864..523973 (+) 1110 WP_248143986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L4174_RS02435 (L4174_002435) - 524186..525502 (+) 1317 WP_248143987.1 YjiH family protein -
  L4174_RS02440 (L4174_002440) ruvX 525651..526079 (-) 429 WP_248143988.1 Holliday junction resolvase RuvX -
  L4174_RS02445 (L4174_002445) - 526076..526639 (-) 564 WP_248143989.1 YqgE/AlgH family protein -
  L4174_RS02450 (L4174_002450) gshB 526783..527733 (-) 951 WP_248143990.1 glutathione synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37929.43 Da        Isoelectric Point: 6.1844

>NTDB_id=705111 L4174_RS02425 WP_248143985.1 521815..522849(+) (pilT) [Photobacterium sp. CCB-ST2H9]
MDITELLDFSVKHNASDLHLSAGVSPMIRVDGDVRALSIPPFSHETVHRLITDIMDDAQRREFEEHLEVDFSFALPDVGR
FRVNAFQQSRGCAAVFRSIPVTVPSLESLGVPEVFYQIARSQRGLVLVTGATGSGKSTTIAALVDHINSLSHRHILTIED
PIEFIHESKRCLINQREVHRDTHSFQNALRSALREDPDVIMVGELRDQETIRLALTAAETGHLVLGTLHTSSAAKAIDRI
IDVFPGNDKAMVRSMLSESLRAVIAQSLLKCPAGGRVAAYEIMLATPAIRNLIREDKVAQMYSMIQTGAGLGMQTMEQAV
NRLLAQGLVEPDEVLRVTERTQGL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=705111 L4174_RS02425 WP_248143985.1 521815..522849(+) (pilT) [Photobacterium sp. CCB-ST2H9]
ATGGATATCACCGAGCTACTGGACTTTAGTGTAAAGCATAATGCCTCCGATCTGCACCTCTCTGCGGGGGTATCGCCGAT
GATCCGGGTGGACGGTGATGTCAGAGCGCTCAGTATCCCGCCTTTCAGCCATGAAACGGTGCACCGGCTGATTACAGATA
TTATGGATGATGCGCAGCGCCGGGAATTTGAAGAGCACCTCGAAGTTGATTTTTCTTTTGCTCTGCCGGATGTCGGACGT
TTCCGGGTCAATGCCTTTCAGCAGTCCCGGGGCTGCGCTGCAGTTTTCCGTTCAATTCCGGTGACGGTTCCGTCACTGGA
ATCGCTGGGTGTTCCTGAAGTGTTTTATCAGATTGCCAGGAGCCAGCGCGGCCTGGTACTGGTCACCGGGGCCACGGGTT
CGGGCAAGTCCACCACGATTGCGGCGCTGGTGGATCACATAAATTCTCTTTCCCACCGTCATATCCTGACGATTGAGGAT
CCGATCGAGTTTATCCATGAAAGCAAACGCTGTCTGATCAATCAGCGCGAAGTCCATCGGGACACCCACAGTTTTCAGAA
TGCCCTGCGTTCGGCGTTGCGGGAAGATCCGGATGTCATTATGGTCGGCGAGCTGCGCGATCAGGAAACCATCCGGCTTG
CGCTGACGGCCGCTGAAACCGGCCATCTGGTGCTGGGCACTCTGCATACCAGCTCGGCGGCGAAAGCGATCGATCGCATC
ATTGATGTCTTCCCCGGGAATGACAAAGCGATGGTCCGTTCCATGCTGTCGGAATCGCTGCGGGCGGTGATTGCACAGTC
GCTGCTGAAATGTCCTGCTGGCGGCAGGGTCGCGGCTTATGAAATCATGCTGGCGACGCCGGCGATCCGTAACCTGATCC
GGGAAGACAAAGTCGCGCAGATGTATTCGATGATCCAGACCGGTGCCGGTCTGGGAATGCAGACCATGGAGCAGGCTGTC
AACCGGCTGCTGGCACAGGGGCTGGTGGAACCCGATGAAGTGCTCCGGGTGACCGAGCGGACTCAGGGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.749

97.093

0.735
  pilT Vibrio cholerae strain A1552

75.749

97.093

0.735
  pilT Pseudomonas stutzeri DSM 10701

69.578

96.512

0.672
  pilT Pseudomonas aeruginosa PAK

69.605

95.64

0.666
  pilT Acinetobacter baumannii D1279779

68.278

96.221

0.657
  pilT Acinetobacter baumannii strain A118

68.278

96.221

0.657
  pilT Acinetobacter nosocomialis M2

67.976

96.221

0.654
  pilT Acinetobacter baylyi ADP1

68.389

95.64

0.654
  pilT Legionella pneumophila strain ERS1305867

66.869

95.64

0.64
  pilT Legionella pneumophila strain Lp02

66.869

95.64

0.64
  pilT Neisseria meningitidis 8013

62.029

100

0.622
  pilT Neisseria gonorrhoeae MS11

61.739

100

0.619
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.381

97.674

0.512
  pilU Pseudomonas stutzeri DSM 10701

42.262

97.674

0.413
  pilU Vibrio cholerae strain A1552

42.262

97.674

0.413
  pilU Acinetobacter baylyi ADP1

41.304

93.605

0.387