Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L4174_RS02430 Genome accession   NZ_CP100425
Coordinates   522864..523973 (+) Length   369 a.a.
NCBI ID   WP_248143986.1    Uniprot ID   -
Organism   Photobacterium sp. CCB-ST2H9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517864..528973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L4174_RS02395 (L4174_002395) - 518058..518651 (-) 594 WP_248143979.1 XTP/dITP diphosphatase -
  L4174_RS02400 (L4174_002400) - 518806..519234 (-) 429 WP_248143980.1 DUF4426 domain-containing protein -
  L4174_RS02405 (L4174_002405) yggU 519359..519652 (-) 294 WP_248143981.1 DUF167 family protein YggU -
  L4174_RS02410 (L4174_002410) - 519649..520206 (-) 558 WP_248143982.1 YggT family protein -
  L4174_RS02415 (L4174_002415) proC 520216..521034 (-) 819 WP_248143983.1 pyrroline-5-carboxylate reductase -
  L4174_RS02420 (L4174_002420) - 521084..521791 (-) 708 WP_248143984.1 YggS family pyridoxal phosphate-dependent enzyme -
  L4174_RS02425 (L4174_002425) pilT 521815..522849 (+) 1035 WP_248143985.1 type IV pilus twitching motility protein PilT Machinery gene
  L4174_RS02430 (L4174_002430) pilU 522864..523973 (+) 1110 WP_248143986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L4174_RS02435 (L4174_002435) - 524186..525502 (+) 1317 WP_248143987.1 YjiH family protein -
  L4174_RS02440 (L4174_002440) ruvX 525651..526079 (-) 429 WP_248143988.1 Holliday junction resolvase RuvX -
  L4174_RS02445 (L4174_002445) - 526076..526639 (-) 564 WP_248143989.1 YqgE/AlgH family protein -
  L4174_RS02450 (L4174_002450) gshB 526783..527733 (-) 951 WP_248143990.1 glutathione synthase -
  L4174_RS02455 (L4174_002455) rsmE 527748..528479 (-) 732 WP_248143991.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40706.60 Da        Isoelectric Point: 6.6353

>NTDB_id=705112 L4174_RS02430 WP_248143986.1 522864..523973(+) (pilU) [Photobacterium sp. CCB-ST2H9]
MELASILAQMAADQASDLYLTVSAPCQLKINGQLSAVGPSLTVDEVERLCQQAMSASQWQTFVATKEANFALVREGHRFR
ISAFWQREQPGMVIRRIESMIPDVDALTLPEPMKAMALAKRGLVLVVGATGSGKSTSMAALTGYRNRNRSGHILTVEDPI
EFIHQHDRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRNQETMEYALNFAETGHLCMATLHANNANQALERILH
LVPRERREQLLFDLSLNLKGILAQQLIPDSHGAGRHAAFELMINTPRISDLIRRNALHELKETMAKGKEQGMQTLDQSLY
ALYQAGKISEADALAHADSANDVRLMIKLGQSGQTASGHSLAGVSIRFD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=705112 L4174_RS02430 WP_248143986.1 522864..523973(+) (pilU) [Photobacterium sp. CCB-ST2H9]
ATGGAACTTGCAAGCATTCTGGCACAAATGGCGGCGGATCAGGCATCGGATCTGTATCTCACGGTTTCTGCACCCTGCCA
GTTGAAAATCAACGGTCAGCTGAGTGCTGTCGGGCCTAGCCTGACTGTTGATGAAGTTGAGCGTTTGTGTCAGCAGGCCA
TGTCAGCCAGTCAATGGCAGACGTTTGTGGCCACGAAAGAAGCTAATTTTGCACTGGTCCGGGAAGGGCATCGTTTCCGT
ATCAGTGCGTTCTGGCAGCGGGAACAGCCGGGCATGGTAATCCGGCGTATCGAGAGCATGATTCCGGATGTCGACGCTCT
GACCCTGCCGGAGCCGATGAAAGCGATGGCGCTGGCCAAGCGCGGACTAGTGCTGGTGGTCGGGGCAACGGGTTCGGGAA
AATCAACTTCAATGGCTGCGCTGACCGGTTACAGAAACCGGAACCGCAGCGGACATATTCTGACCGTCGAAGATCCGATT
GAATTTATTCATCAGCACGACCGTTGTATCGTGACCCAGCGTGAAGTCGGACTGGATACCGAAAGCTATGAAGTCGCCCT
GAAAAACTCCCTGCGGCAGGCGCCGGATATGATCCTGATCGGTGAGATCCGAAACCAGGAAACCATGGAATATGCCCTGA
ACTTTGCAGAAACCGGCCATCTTTGCATGGCAACTCTGCATGCAAACAATGCCAATCAGGCACTGGAGCGGATCCTGCAT
CTGGTGCCCAGGGAACGGCGGGAGCAGTTGTTGTTCGATTTATCCCTGAATCTGAAAGGGATCCTGGCGCAGCAGTTGAT
CCCGGACAGTCACGGCGCCGGGCGTCATGCTGCCTTTGAGCTGATGATCAATACCCCCAGAATTTCGGATCTGATCCGAA
GAAATGCCCTGCATGAGCTGAAAGAAACGATGGCCAAAGGAAAAGAGCAGGGCATGCAGACTTTAGATCAGTCTTTGTAT
GCATTGTATCAGGCCGGAAAGATCAGTGAAGCGGACGCTTTGGCCCATGCCGATTCTGCCAATGATGTCCGGTTGATGAT
CAAACTGGGTCAGTCCGGTCAGACCGCATCCGGGCATAGTCTGGCGGGAGTCTCCATTCGTTTTGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

66.393

99.187

0.659
  pilU Pseudomonas stutzeri DSM 10701

55.858

99.458

0.556
  pilU Acinetobacter baylyi ADP1

54.237

95.935

0.52
  pilT Legionella pneumophila strain Lp02

40.299

90.786

0.366
  pilT Legionella pneumophila strain ERS1305867

40.299

90.786

0.366
  pilT Acinetobacter baumannii D1279779

40.181

89.702

0.36
  pilT Acinetobacter nosocomialis M2

40.181

89.702

0.36
  pilT Acinetobacter baumannii strain A118

40.181

89.702

0.36
  pilT Pseudomonas aeruginosa PAK

39.701

90.786

0.36