Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFC98_RS01030 Genome accession   NZ_CP100360
Coordinates   211074..212108 (+) Length   344 a.a.
NCBI ID   WP_183330980.1    Uniprot ID   -
Organism   Halomonas sp. 3H     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 206074..217108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFC98_RS01000 - 206487..206942 (-) 456 WP_254276401.1 DUF4426 domain-containing protein -
  NFC98_RS01005 metW 206972..207568 (-) 597 WP_183330975.1 methionine biosynthesis protein MetW -
  NFC98_RS01010 - 207565..208722 (-) 1158 WP_254276402.1 homoserine O-acetyltransferase -
  NFC98_RS01015 - 208766..209359 (-) 594 WP_254276403.1 YggT family protein -
  NFC98_RS01020 proC 209420..210241 (-) 822 WP_254276404.1 pyrroline-5-carboxylate reductase -
  NFC98_RS01025 - 210304..211011 (-) 708 WP_254276405.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFC98_RS01030 pilT 211074..212108 (+) 1035 WP_183330980.1 type IV pilus twitching motility protein PilT Machinery gene
  NFC98_RS01035 pilU 212157..213299 (+) 1143 WP_254276406.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NFC98_RS01040 - 213315..214454 (-) 1140 WP_254276407.1 anhydro-N-acetylmuramic acid kinase -
  NFC98_RS01045 tyrS 214571..215770 (+) 1200 WP_254276408.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37921.48 Da        Isoelectric Point: 6.3149

>NTDB_id=704742 NFC98_RS01030 WP_183330980.1 211074..212108(+) (pilT) [Halomonas sp. 3H]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDIMADRQRRDYEEFFETDFSFEVPGVSR
FRVNVFNQARGAGAVFRTIPSEVLTLEDLGLGTVFRQLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYEHILTIED
PVEFVHRSKRCLINQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVISQTLLKRMGGGRVAAHEILIATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLDASL
AKLVADNVVARDEAQAKAKGVLAS

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=704742 NFC98_RS01030 WP_183330980.1 211074..212108(+) (pilT) [Halomonas sp. 3H]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCAGAACGCCTCCGACCTCCATCTCTCGGCGGGCCTGCCGCCGAT
GATTCGCGTGGACGGCGATATCCGCCGGCTCAACGTGCCGGCCATGGAGGACCGCGAGGTTCGCAAGCTGATCTACGACA
TCATGGCCGATCGGCAGCGCCGCGACTACGAGGAGTTCTTCGAGACCGACTTCTCCTTCGAGGTGCCCGGGGTGTCGCGC
TTCCGTGTCAACGTGTTCAACCAGGCGCGCGGTGCCGGGGCGGTGTTCCGTACCATTCCCTCCGAGGTGCTGACCCTGGA
GGACCTGGGCCTCGGCACCGTCTTCCGCCAGCTCTCGATGCTGCCGCGCGGGCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGAGCACCACGCTGGCGGCGATGATCGACTACATCAACGACAACCGCTACGAGCACATCCTGACCATCGAGGAT
CCGGTGGAATTCGTCCACCGCAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCATCGCGATACCCACGGCTTCGCCCC
GGCGCTGCGCAGTGCCCTGCGCGAGGACCCGGACGTGATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATCCGGCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGCGAGGAAAAGTCCATGGTGCGCTCGATGCTCTCGGAGTCGCTGCAGGCGGTGATCTCCCAGAC
GCTGCTCAAGCGCATGGGCGGCGGGCGCGTGGCGGCCCACGAGATCCTCATCGCCACCGCGGCGGTGCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATCTATTCCGCCATCCAGACCGGCGGCAACCTGGGCATGCAGACCCTGGACGCTTCGCTG
GCCAAGCTGGTGGCCGACAACGTGGTGGCGCGTGACGAGGCGCAGGCCAAGGCGAAGGGCGTGCTGGCGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.941

98.547

0.788
  pilT Pseudomonas aeruginosa PAK

79.056

98.547

0.779
  pilT Acinetobacter baumannii D1279779

78.171

98.547

0.77
  pilT Acinetobacter baumannii strain A118

78.171

98.547

0.77
  pilT Acinetobacter nosocomialis M2

77.876

98.547

0.767
  pilT Acinetobacter baylyi ADP1

77.581

98.547

0.765
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

66.276

99.128

0.657
  pilT Neisseria gonorrhoeae MS11

66.176

98.837

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.097

98.547

0.523
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

40.615

94.477

0.384