Detailed information NTG0704743

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NFC98_RS01035
• Genome accession: NZ_CP100360
• Coordinates: 212157..213299 (+)
• Length: 380 a.a.
• NCBI ID: WP_254276406.1
• Uniprot ID: -
• Organism: Halomonas sp. 3H
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 207157..218299

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NFC98_RS01010	-	207565..208722 (-)	1158	WP_254276402.1	homoserine O-acetyltransferase	-
NFC98_RS01015	-	208766..209359 (-)	594	WP_254276403.1	YggT family protein	-
NFC98_RS01020	proC	209420..210241 (-)	822	WP_254276404.1	pyrroline-5-carboxylate reductase	-
NFC98_RS01025	-	210304..211011 (-)	708	WP_254276405.1	YggS family pyridoxal phosphate-dependent enzyme	-
NFC98_RS01030	pilT	211074..212108 (+)	1035	WP_183330980.1	type IV pilus twitching motility protein PilT	Machinery gene
NFC98_RS01035	pilU	212157..213299 (+)	1143	WP_254276406.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NFC98_RS01040	-	213315..214454 (-)	1140	WP_254276407.1	anhydro-N-acetylmuramic acid kinase	-
NFC98_RS01045	tyrS	214571..215770 (+)	1200	WP_254276408.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42372.66 Da
• Isoelectric Point: 6.0956

>NTDB_id=704743 NFC98_RS01035 WP_254276406.1 212157..213299(+) (pilU) [Halomonas sp. 3H]
MTAKEWLYQLLDIMVQKEASDLLISVNAPPTLKMAGKLTPLGDQGLSVAQVDELVSASIPDVVMERFRVEREANFALSLK
DKGRFRVSAFQQRNQKAMVIRRIAFEIPHLEELSLPSVLGELANNKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHKKAIINQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNLRAIVAQQLLPTVDGGRCPAIEIMLKSPLIGDLVRKGEVNEIKNVMARSRDLGMQTF
DQALYDLFRAGKITEEVALVHADSANDLRMMIKYGDEGSEGMTQAREAASHLSLRRDDDF

Nucleotide

• Length: 1143 bp

>NTDB_id=704743 NFC98_RS01035 WP_254276406.1 212157..213299(+) (pilU) [Halomonas sp. 3H]
ATGACGGCGAAAGAGTGGCTTTACCAGCTGCTGGACATCATGGTGCAGAAGGAAGCCTCGGATCTGCTGATCTCGGTGAA
TGCGCCGCCGACCTTGAAGATGGCCGGGAAGCTCACCCCGCTGGGGGATCAGGGGCTCTCCGTGGCCCAGGTCGACGAGC
TGGTCAGCGCGTCGATTCCGGACGTGGTGATGGAGCGCTTCCGGGTGGAGCGCGAGGCCAACTTCGCACTGAGCCTCAAG
GACAAGGGGCGCTTCCGTGTGAGCGCCTTCCAGCAGCGTAACCAGAAGGCGATGGTGATTCGCCGCATCGCCTTCGAGAT
CCCCCACCTGGAGGAGCTGTCGCTGCCCTCGGTGCTGGGCGAGCTGGCCAACAACAAGCGCGGCCTGGTGTTCGTCGTGG
GCGGTACCGGTACCGGCAAGTCGACCACCCTGGCGTCAATGATCCAGCAGCGCAACGAGACCCTGGGCGGGCATATCATC
AGCGTCGAGGACCCCATCGAGTACGTGCATCCGCACAAGAAGGCGATCATCAACCAGCGCGAGGTGGGGATCGACACCGA
CTCCTTCGAGGTGGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGGACGCGGGAGA
CCATGGAGCATGCGCTGACCTTCGCCGAGACCGGTCACCTCTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCA
CTGGACCGCATCATCCACTTCTTCCCCCATGAGCGGCACGAGCAGATCTGGCTCGACCTGTCGCTCAACCTCCGGGCAAT
CGTCGCCCAGCAGCTGCTGCCCACCGTGGACGGCGGGCGCTGCCCGGCCATCGAGATCATGCTCAAGTCGCCGCTGATCG
GCGACCTGGTGCGCAAGGGCGAGGTCAACGAGATCAAGAACGTGATGGCCCGCTCCCGGGACCTGGGCATGCAGACCTTC
GACCAGGCCCTGTACGACCTCTTCAGGGCGGGCAAGATCACCGAGGAGGTGGCGCTGGTGCATGCCGACTCGGCCAATGA
CCTGCGCATGATGATCAAGTACGGCGACGAGGGCAGCGAGGGGATGACCCAGGCCCGCGAGGCGGCCAGCCACCTCTCGC
TGCGCCGCGACGACGACTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.322	98.158	0.592
pilU	Vibrio cholerae strain A1552	59.003	95	0.561
pilU	Acinetobacter baylyi ADP1	55.493	93.421	0.518
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.837	100	0.432
pilT	Legionella pneumophila strain ERS1305867	41.617	87.895	0.366
pilT	Legionella pneumophila strain Lp02	41.617	87.895	0.366
pilT	Neisseria meningitidis 8013	40.588	89.474	0.363
pilT	Neisseria gonorrhoeae MS11	39.942	90.263	0.361