Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NHM04_RS12390 Genome accession   NZ_CP100350
Coordinates   2821012..2822046 (+) Length   344 a.a.
NCBI ID   WP_020210690.1    Uniprot ID   A0A9J7A541
Organism   Gilvimarinus sp. DA14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2816012..2827046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHM04_RS12370 (NHM04_12370) - 2816612..2818609 (-) 1998 WP_254264098.1 dynamin family protein -
  NHM04_RS12375 (NHM04_12375) - 2818774..2819364 (-) 591 WP_254264099.1 YggT family protein -
  NHM04_RS12380 (NHM04_12380) proC 2819385..2820203 (-) 819 WP_256526535.1 pyrroline-5-carboxylate reductase -
  NHM04_RS12385 (NHM04_12385) - 2820200..2820922 (-) 723 WP_254264100.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHM04_RS12390 (NHM04_12390) pilT 2821012..2822046 (+) 1035 WP_020210690.1 type IV pilus twitching motility protein PilT Machinery gene
  NHM04_RS12395 (NHM04_12395) pilU 2822135..2823280 (+) 1146 WP_254264101.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NHM04_RS12400 (NHM04_12400) - 2823573..2825279 (+) 1707 WP_254264102.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38253.99 Da        Isoelectric Point: 6.5053

>NTDB_id=704701 NHM04_RS12390 WP_020210690.1 2821012..2822046(+) (pilT) [Gilvimarinus sp. DA14]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKQVHSLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGQVFRDICAVPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHVLTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKAMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGASVGMQTMDQCL
EDLVAKRVISREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=704701 NHM04_RS12390 WP_020210690.1 2821012..2822046(+) (pilT) [Gilvimarinus sp. DA14]
ATGGATATTACGGAGCTCCTCGCCTTTAGCGCCAAACAGGGCGCTTCAGACTTGCACCTTTCGGCCGGTTTGCCGCCCAT
GATTCGGGTAGATGGGGATGTACGGCGGATTAACCTGCCCCCTATGGACCACAAACAGGTGCATAGTTTGATCTACGAGA
TCATGAATGACAAGCAGCGCAAGGACTACGAAGAGTTCCTGGAAACCGACTTCTCTTTTGAAGTGCCGGGTGTGGCCCGC
TTCCGGGTAAACGCCTTTAACCAAAACCGCGGCGCCGGCGCGGTGTTCCGTACCATTCCCTCTAAAGTACTCACCATGGA
AGAGCTGGGCATGGGCCAGGTGTTTCGCGATATCTGCGCGGTACCGCGCGGTTTGGTACTGGTCACCGGCCCCACGGGCT
CGGGTAAATCCACCACCCTTGCCGCCATGATGGACTACATCAACGACAACAAATACGAACACGTACTGACCATCGAAGAC
CCCATCGAATTTGTGCACGAATCGAAAAAGTGCCTGGTCAACCAGCGTGAAGTGCACCGCGATACCCACGGCTTTGCCGA
AGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATTATTCTGGTAGGTGAGATGCGTGATTTGGAAACCATTCGCCTGG
CGCTGACTGCCGCCGAAACCGGCCACTTGGTATTCGGCACCTTGCACACCACCAGCGCCGCCAAAACCATTGACCGGGTG
GTGGATGTATTCCCGGCCAATGAAAAAGCCATGGTGCGCTCCATGTTGTCGGAATCGCTGCAGGCAGTAATCTCGCAAAC
ACTCATGAAGAAAAATGGCGGCGGCCGGGTCGCGGCCCACGAAATTATGCGCGGCACACCGGCCATTCGTAACCTGATTC
GCGAAGACAAAGTCGCGCAGATGTATTCGGCCATTCAAACCGGTGCCTCTGTAGGTATGCAAACCATGGATCAGTGCCTG
GAAGATCTGGTGGCCAAGCGCGTTATCTCTCGCGAAGTAGCGCGCGAAAAAGCCAAGATGCCAGAGAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

90.116

100

0.901
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.413

98.547

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384